解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749658
更新日期:1995-10-01 00:00:00
abstract::This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749764
更新日期:1995-06-01 00:00:00
abstract::A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749221
更新日期:1995-02-01 00:00:00
abstract::Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744313
更新日期:1995-01-01 00:00:00
abstract::We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744323
更新日期:1995-01-01 00:00:00
abstract::A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749758
更新日期:1995-01-01 00:00:00
abstract::A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00772193
更新日期:1994-01-01 00:00:00
abstract::The growth yields for glucose and glutamine of murine hybridoma cells entrapped in collagen gel particles were examined during the growth phase. The immobilized hybridoma cells were cultivated in a fluidized bed fermenter where the medium was circulating to supply oxygen separately. Procedures to supply an alkaline so...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00758178
更新日期:1994-01-01 00:00:00
abstract::"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00758179
更新日期:1994-01-01 00:00:00
abstract::Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF00749616
更新日期:1994-01-01 00:00:00
abstract::Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00761778
更新日期:1994-01-01 00:00:00
abstract::The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00754609
更新日期:1994-01-01 00:00:00
abstract::The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model system. A variety of monitoring techniques including trypan blue ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762392
更新日期:1994-01-01 00:00:00
abstract::In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762402
更新日期:1994-01-01 00:00:00
abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762410
更新日期:1994-01-01 00:00:00
abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749004
更新日期:1993-01-01 00:00:00
abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF00744659
更新日期:1993-01-01 00:00:00
abstract::Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749933
更新日期:1993-01-01 00:00:00
abstract::Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749813
更新日期:1993-01-01 00:00:00
abstract::The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Iodine labeled metaiodobenzylguanidine (MIBG) is a radiopharmaceutical employed for both diagnosis and metabolic radiotherapy of neuroblastoma (NB). Resistance to the radiotherapeutic effects of MIBG is common, due to lack of MIBG accumulation by NB cells. MIBG enters competent cells via the noradrenaline transporter;...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746080
更新日期:1993-01-01 00:00:00
abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques.Applications concern the setting of uptake-effect relationships a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746046
更新日期:1993-01-01 00:00:00
abstract::The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746055
更新日期:1993-01-01 00:00:00
abstract::A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746064
更新日期:1993-01-01 00:00:00
abstract::Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/BF00744670
更新日期:1993-01-01 00:00:00
abstract::Low-frequency dielectric spectroscopy has been used in situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is cha...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749815
更新日期:1993-01-01 00:00:00
abstract::The Chinook salmon embryo cell line, CHSE-214, grew well in fetal bovine serum (FBS) but poorly in dialyzed (d) FBS. Purines restored most but not all growth-promoting activity to dFBS, which suggests that purines account for a large portion of the dialyzable fraction's growth-promoting activity. CHSE-214 died in newb...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02540026
更新日期:1992-01-01 00:00:00
abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02525492
更新日期:1992-01-01 00:00:00
abstract::The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02522040
更新日期:1992-01-01 00:00:00
abstract::We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02521744
更新日期:1992-01-01 00:00:00
abstract::This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02521730
更新日期:1992-01-01 00:00:00
abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00570891
更新日期:1992-01-01 00:00:00
abstract::Cell cycle, cell size and rhodamine 123 fluorescence in cell populations of two batch cultures were analysed and quantified with a fluorescence-activated cell sorter (FACS). Two cultures derived from either exponential or stationary phase innocula were investigated in order to demonstrate the dependency of the subsequ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365929
更新日期:1991-11-01 00:00:00
abstract::Previously, it has been shown that the binding of epidermal growth factor (EGF) by a wide range of cells decreases as cell density increases. In this report, we demonstrate that KB cells treated chronically with phorbol esters continue to exhibit decreases in EGF receptor binding as cell density increases. This findin...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00350914
更新日期:1991-10-01 00:00:00
abstract::Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00135638
更新日期:1991-09-01 00:00:00
abstract::Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353702
更新日期:1991-05-01 00:00:00
abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00556297
更新日期:1991-03-01 00:00:00
abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736794
更新日期:1991-02-01 00:00:00