abstract：:There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

journal_title：Cytotechnology

authors： Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

更新日期：2021-02-01 00:00:00

abstract：:Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...

journal_title：Cytotechnology

authors： Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

更新日期：2021-02-01 00:00:00

abstract：:MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

journal_title：Cytotechnology

authors： Tan PY,Wen LJ,Li HN,Chai SW

更新日期：2020-10-01 00:00:00

abstract：:In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...

journal_title：Cytotechnology

authors： Seong JH,Song YS,Joo HW,Park IH,Shen GY,Shin NK,Lee AH,Kwon AM,Lee Y,Kim H,Kim KS

更新日期：2020-10-01 00:00:00

abstract：:Human umbilical cord-derived mesenchymal stem cells (hUMSCs) hold strong self-renewal capacity and low immunogenicity, which have attracted attention as potential candidates for bone repair and regeneration. However, insufficient osteogenic differentiation markedly hinders the clinical applications of hUMSCs. In the p...

journal_title：Cytotechnology

authors： Liu J,Wang H,Ren W,Zhou Y,Ye Z,Tan WS

更新日期：2020-10-01 00:00:00

abstract：:Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...

journal_title：Cytotechnology

authors： Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

更新日期：2020-06-01 00:00:00

abstract：:Increasing interest has been observed in the use of photobiomodulation (PBM) to enhance the proliferation of stem cells and induce their differentiation. The effects of PBM at two different wavelengths (635 and 809 nm) with three different energy densities (0.5, 1 and 2 J/cm2) on the osteogenic differentiation of adip...

journal_title：Cytotechnology

authors： Bölükbaşı Ateş G,Ak A,Garipcan B,Gülsoy M

更新日期：2020-04-01 00:00:00

abstract：:Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...

journal_title：Cytotechnology

authors： Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

更新日期：2020-04-01 00:00:00

abstract：:Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...

journal_title：Cytotechnology

authors： Weng Z,Jin J,Shao C,Li H

更新日期：2020-04-01 00:00:00

abstract：:Oxidative stress plays a vital role in the occurrence and development of intestinal injury. Soluble epoxide hydrolase 2 gene (EPHX2) is a class of hydrolytic enzymes. We aim to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells. IEC-6 cells ...

journal_title：Cytotechnology

authors： Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

更新日期：2020-02-01 00:00:00

abstract：:Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...

journal_title：Cytotechnology

authors： Chen P,Demirji J,Ivleva VB,Horwitz J,Schwartz R,Arnold F

更新日期：2019-12-01 00:00:00

abstract：:Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and ...

journal_title：Cytotechnology

authors： Timmins LM,Patel RS,Teryek MS,Parekkadan B

更新日期：2019-10-01 00:00:00

abstract：:Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...

journal_title：Cytotechnology

authors： Bashokouh F,Abbasiliasi S,Tan JS

更新日期：2019-07-16 00:00:00

abstract：:Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

journal_title：Cytotechnology

authors： Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

更新日期：2019-07-12 00:00:00

abstract：:To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...

journal_title：Cytotechnology

authors： Wu Z,Liu Y,Huang J,Huang Y,Fan L

更新日期：2019-06-26 00:00:00

abstract：:The aim of this study was to reveal the biological activities and in vivo toxicity profiles of n-hexane, chloroform and methanol extracts of brown algae Halopteris scoparia L. Sauvageau. In this study, extracts were tested for their phytochemical contents and antioxidant activities. The cytotoxic activities of the ext...

journal_title：Cytotechnology

authors： Güner A,Nalbantsoy A,Sukatar A,Karabay Yavaşoğlu NÜ

更新日期：2019-06-01 00:00:00

abstract：:The present study describes an immunocytochemistry (ICC) assay with self-raised hyperimmune sera and a Baby Hamster Kidney-21 (BHK-21) cell line infected with Porcine Sapelovirus (PSV). Sapelovivus/IVRI/SPF-c-6/2015 strain Indian PSV was isolated from the porcine IBRS-2 cell line and investigated for growth on non-por...

journal_title：Cytotechnology

authors： Kumari S,Singh R,Desingu PA,Ray PK,Taru Sharma G,Saikumar G

更新日期：2019-06-01 00:00:00

abstract：:Hyperuricemia is an important risk factor for gout. Isorhamnetin (3'-O-methylquercetin) is an O-methylated flavonol, which occurs in onion, almond and sea buckthorn. It is also one of the metabolites of quercetin in mammals. In the present study, we investigated anti-hyperuricemic effect of isorhamnetin adopting both ...

journal_title：Cytotechnology

authors： Adachi SI,Kondo S,Sato Y,Yoshizawa F,Yagasaki K

更新日期：2019-02-01 00:00:00

abstract：:Nanotechnology is increasingly developing area including more than 700 commercial products such as clothing, food preparation, cosmetics, mechanics, electronics and also health industry. People generally contact with nanoparticles by inhaling from air. Thus, it is becoming important issue to understand harmful effects...

journal_title：Cytotechnology

authors： Türkez H,Arslan ME,Sönmez E,Açikyildiz M,Tatar A,Geyikoğlu F

更新日期：2019-02-01 00:00:00

abstract：:Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...

journal_title：Cytotechnology

authors： Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

更新日期：2019-02-01 00:00:00

abstract：:Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver...

journal_title：Cytotechnology

authors： Mogalli R,Matsukawa T,Shimomura O,Isoda H,Ohkohchi N

更新日期：2018-12-01 00:00:00

abstract：:The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...

journal_title：Cytotechnology

authors： Rastgar S,Movahedinia A,Salamat N,Salati A,Zabihi E

更新日期：2018-12-01 00:00:00

abstract：:We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...

journal_title：Cytotechnology

authors： Xu Z,Yu L,Lu H,Feng W,Chen L,Zhou J,Yang X,Qi Z

更新日期：2018-12-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...

journal_title：Cytotechnology

authors： Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

更新日期：2018-12-01 00:00:00

abstract：:Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...

journal_title：Cytotechnology

authors： Eugenio M,Campanati L,Müller N,Romão LF,de Souza J,Alves-Leon S,de Souza W,Sant'Anna C

更新日期：2018-12-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...

journal_title：Cytotechnology

authors： Feng W,Liang C,Wang C,Yu X,Li Q,Yang H

更新日期：2018-10-01 00:00:00

abstract：:Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...

journal_title：Cytotechnology

authors： Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

更新日期：2018-10-01 00:00:00

abstract：:Although a variety of assays have been used to examine T cell responses in vitro, standardized ex vivo detection of antigen-specific CD4+ T cells from human circulatory PBMCs remains constrained by low-dimensional characterization outputs and the need for polyclonal, mitogen-induced expansion methods to generate detec...

journal_title：Cytotechnology

authors： Carrio R,Zhang G,Drake DR 3rd,Schanen BC

更新日期：2018-10-01 00:00:00

abstract：:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...

journal_title：Cytotechnology

authors： He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

更新日期：2018-08-01 00:00:00

abstract：:Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...

journal_title：Cytotechnology

authors： Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

更新日期：2018-06-01 00:00:00

abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...

journal_title：Cytotechnology

authors： Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

更新日期：2018-06-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs), one type of mesenchymal stem cells, are considered to be a type of tool cells for regenerative medicine and tissue engineering. Our previous studies found that the stimulation with lipopolysaccharide (LPS) might introduce senescence of DPSCs, and this senescence would have a positive co...

journal_title：Cytotechnology

authors： Feng G,Zheng K,Cao T,Zhang J,Lian M,Huang D,Wei C,Gu Z,Feng X

更新日期：2018-06-01 00:00:00

abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...

journal_title：Cytotechnology

authors： Ribeiro DL,Cilião HL,Specian AFL,Serpeloni JM,De Oliveira MT,Varanda EA,Vilegas W,Saldanha LL,Martínez-López W,Dokkedal AL,Cólus IMS

更新日期：2018-04-01 00:00:00

abstract：:Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...

journal_title：Cytotechnology

authors： Ma Y,Guo Y,Wu S,Lv Z,Zhang Q,Xie X,Ke Y

更新日期：2018-02-01 00:00:00

abstract：:Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...

journal_title：Cytotechnology

authors： Tao H,Nuo M,Min S

更新日期：2018-02-01 00:00:00

abstract：:Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...

journal_title：Cytotechnology

authors： Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

更新日期：2018-02-01 00:00:00

abstract：:Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

journal_title：Cytotechnology

authors： Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

更新日期：2017-12-01 00:00:00

abstract：:The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...

journal_title：Cytotechnology

authors： Peng G,Xu J,Liu R,Fu Z,Li S,Hong W,Chen J,Li B,Ran P

更新日期：2017-10-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) are key players in liver fibrosis and regeneration via collagen degradation and synthesis. These phenomena involve inflammatory cytokines released from non-parenchymal liver cells such as Kupffer cells. Although the effects of individual cytokines on many cell types have been investigated...

journal_title：Cytotechnology

authors： Inoue A,Obayashi K,Sonoda Y,Nakamura A,Ueno T,Kuhara S,Tashiro K

更新日期：2017-06-01 00:00:00