Isolation, culture and identification of pulmonary arterial smooth muscle cells from rat distal pulmonary arteries.

abstract：:The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...

journal_title：Cytotechnology

authors： Theise ND

更新日期：2003-03-01 00:00:00

abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...

journal_title：Cytotechnology

authors： Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

更新日期：2016-08-01 00:00:00

abstract：:Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...

journal_title：Cytotechnology

authors： Dufresne M,Warocquier-Clérout R

更新日期：2001-09-01 00:00:00

abstract：:Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...

journal_title：Cytotechnology

authors： Hashimoto K,Shimizu M

更新日期：1993-01-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...

journal_title：Cytotechnology

authors： Yagi Y,Ito Y,Kuhara S,Tashiro K

更新日期：2008-06-01 00:00:00

abstract：:Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...

journal_title：Cytotechnology

authors： Azimi M,Niimi T,Yoshida N,Kitagawa Y

更新日期：1999-09-01 00:00:00

abstract：:Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...

journal_title：Cytotechnology

authors： Sahraneshin Samani F,Moore JK,Khosravani P,Ebrahimi M

更新日期：2014-08-01 00:00:00

abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...

journal_title：Cytotechnology

authors： Ribeiro DL,Cilião HL,Specian AFL,Serpeloni JM,De Oliveira MT,Varanda EA,Vilegas W,Saldanha LL,Martínez-López W,Dokkedal AL,Cólus IMS

更新日期：2018-04-01 00:00:00

abstract：:A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...

journal_title：Cytotechnology

authors： Isoda H,Shinmoto H,Matsumura M,Nakahara T

更新日期：1995-01-01 00:00:00

abstract：:For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...

journal_title：Cytotechnology

authors： Goergen JL,Marc A,Engasser JM

更新日期：1992-01-01 00:00:00

abstract：:In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...

journal_title：Cytotechnology

authors： Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux M

更新日期：2016-10-01 00:00:00

abstract：:The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...

journal_title：Cytotechnology

authors： Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

更新日期：2008-09-01 00:00:00

abstract：:Rats subcutaneously implanted with AH109A hepatoma cells show hyperlipidemia with high concentrations of serum triglyceride and nonesterified fatty acid, suppression of lipoprotein lipase (LPL), and elevation of hormone-sensitive lipase (HSL) activities during the growth of the hepatoma. Supplementation of the diet wi...

journal_title：Cytotechnology

authors： Kawasaki M,Miura Y,Yagasaki K

更新日期：2010-07-01 00:00:00

abstract：:A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...

journal_title：Cytotechnology

authors： Escudero T,Coll MD,Fuster C,Egozcue J

更新日期：2002-09-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:Previously, it has been shown that the binding of epidermal growth factor (EGF) by a wide range of cells decreases as cell density increases. In this report, we demonstrate that KB cells treated chronically with phorbol esters continue to exhibit decreases in EGF receptor binding as cell density increases. This findin...

journal_title：Cytotechnology

authors： Rizzino A,Huebert C,Kuszynski C,Wilder PJ

更新日期：1991-10-01 00:00:00

abstract：:MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

journal_title：Cytotechnology

authors： Tan PY,Wen LJ,Li HN,Chai SW

更新日期：2020-10-01 00:00:00

abstract：:Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...

journal_title：Cytotechnology

authors： Chromikova V,Mader A,Steinfellner W,Kunert R

更新日期：2015-03-01 00:00:00

abstract：:Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...

journal_title：Cytotechnology

authors： Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

更新日期：2008-09-01 00:00:00

abstract：:The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented w...

journal_title：Cytotechnology

authors： Sadeesh EM,Selokar NL,Balhara AK,Yadav PS

更新日期：2016-10-01 00:00:00

abstract：:Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...

journal_title：Cytotechnology

authors： Miller K,Wilder PJ,Rizzino A

更新日期：1993-01-01 00:00:00

abstract：:We have previously demonstrated the radio-sensitizing effect of zoledronic acid (ZOL), a third generation bisphosphonate, on human esophageal squamous cell carcinoma (ESCC) cells. Here we show that ZOL suppresses metastatic progression of ESCC cells mainly through up-regulating the tight junction protein occludin. Exp...

journal_title：Cytotechnology

authors： Lin C,Xin S,Qin X,Li H,Lin L,You Y

更新日期：2016-08-01 00:00:00

abstract：CONCLUSIONS:With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from in...

journal_title：Cytotechnology

authors： Lynn DE

更新日期：1996-01-01 00:00:00

abstract：:Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...

journal_title：Cytotechnology

authors： Xiu ZL,Deckwer WD,Zeng AP

更新日期：1999-05-01 00:00:00

abstract：:Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...

journal_title：Cytotechnology

authors： Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

更新日期：2003-11-01 00:00:00

abstract：:A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...

journal_title：Cytotechnology

authors： Lee HJ,Kang HY,Kim CH,Kim HS,Kwon MC,Kim SM,Shin IS,Lee HY

更新日期：2006-11-01 00:00:00

abstract：:Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...

journal_title：Cytotechnology

authors： Solmi R,Rossetti A,Talassi O,Tomasini GL,Fato R,Estornell E,Lucarini G,Lenaz G,Simonelli L,Brunelli MA

更新日期：1993-01-01 00:00:00

abstract：:Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a poly...

journal_title：Cytotechnology

authors： Karaman E,Urhan Kucuk M,Bayramoglu A,Uzun Göçmen S,Ercan S,Guler HI,Kucukkaya Y,Erden S

更新日期：2015-12-01 00:00:00

abstract：:Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

journal_title：Cytotechnology

authors： Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

更新日期：2019-07-12 00:00:00