Abstract:
:Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of CK, LDH, MDA and SOD, and the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were measured. The levels of total and phosphorylated ERK1/2, JNK, and p38 were measured by western blotting in the heart, and the myocardial H9C2 cell line was studied. Using the Cell Counting Kit-8, the growth rate of H9C2 cells affected by sufentanil was studied. The serum levels of CK, LDH and MDA were higher in the IR group than in the SO and SUF groups. The SOD level, as well as the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase, were lower in the SO and SUF groups than in the IR group. The phosphorylated ERK1/2 level was lower in the IR group than in the SO and SUF groups. The growth rate of H9C2 cells increased with the concentration of sufentanil and the exposure time. The phosphorylated ERK level was upregulated by 4-12 h of sufentanil exposure, indicating that the effects were time-dependent. Furthermore, an inhibition of ERK signaling by chemical inhibition suppressed the sufentanil-mediated increase in the growth rate of H9C2 cells. Sufentanil appears to be beneficial for cases of worsening ischemic heart disease. Further studies are necessary before a clinical application is considered.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Tao H,Nuo M,Min Sdoi
10.1007/s10616-017-0127-ysubject
Has Abstractpub_date
2018-02-01 00:00:00pages
169-176issue
1eissn
0920-9069issn
1573-0778pii
10.1007/s10616-017-0127-yjournal_volume
70pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749004
更新日期:1993-01-01 00:00:00
abstract::Achyranthes bidentata (A. bidentata) Blume is a medicinal herb with the property of strengthening bones and muscles and ensuring proper downward flow of blood in terms of the therapeutic theory of traditional medicine. In the present study, the effect of A. bidentata root extract (AE) on osteoblast function was invest...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9645-4
更新日期:2014-12-01 00:00:00
abstract::Iodine labeled metaiodobenzylguanidine (MIBG) is a radiopharmaceutical employed for both diagnosis and metabolic radiotherapy of neuroblastoma (NB). Resistance to the radiotherapeutic effects of MIBG is common, due to lack of MIBG accumulation by NB cells. MIBG enters competent cells via the noradrenaline transporter;...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00746080
更新日期:1993-01-01 00:00:00
abstract::In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9626-7
更新日期:2014-10-01 00:00:00
abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02525492
更新日期:1992-01-01 00:00:00
abstract::The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among t...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008166310368
更新日期:2000-02-01 00:00:00
abstract::Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9082-3
更新日期:2007-06-01 00:00:00
abstract::In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762402
更新日期:1994-01-01 00:00:00
abstract::Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00330-5
更新日期:2019-07-16 00:00:00
abstract::The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model system. A variety of monitoring techniques including trypan blue ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762392
更新日期:1994-01-01 00:00:00
abstract::The cAMP and Ca(2+) signaling pathways activate the transcription factor CREB through its phosphorylation at Serine 133. Activation of CREB is involved in the regulation of various biological phenomena. To understand further the mechanisms of the regulation of CREB activity in response to activation of the cAMP and Ca...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3763-6
更新日期:2005-01-01 00:00:00
abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00762410
更新日期:1994-01-01 00:00:00
abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9995-9
更新日期:2016-12-01 00:00:00
abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9734-z
更新日期:2015-12-01 00:00:00
abstract::Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9795-z
更新日期:2016-05-01 00:00:00
abstract::A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749221
更新日期:1995-02-01 00:00:00
abstract::The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of act...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9814-0
更新日期:2016-08-01 00:00:00
abstract::Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008005200711
更新日期:1999-07-01 00:00:00
abstract::The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0256-y
更新日期:2018-12-01 00:00:00
abstract::Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008058031511
更新日期:1998-09-01 00:00:00
abstract::The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-4637-7
更新日期:2005-09-01 00:00:00
abstract::Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0253-1
更新日期:2018-12-01 00:00:00
abstract::We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0262-0
更新日期:2018-12-01 00:00:00
abstract::Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated dise...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0047-2
更新日期:2017-02-01 00:00:00
abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736794
更新日期:1991-02-01 00:00:00
abstract::It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9158-8
更新日期:2008-07-01 00:00:00
abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW co...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365080
更新日期:1988-11-01 00:00:00
abstract::Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the pr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353929
更新日期:1996-01-01 00:00:00
abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9191-2
更新日期:2009-01-01 00:00:00