Abstract:
:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere goat hair follicle, and also positively expressed in both nucleus and cytoplasm of the gHFSCs. When the cells were transfected using Sox9-shRNA, cell growth slowed down and the expression of related genes decreased significantly, cell cycle was abnormal, while the expression of terminal differentiation marker loricrin was markedly increased; cells lost the typical morphology of HFSCs; the mRNA and protein expression of gHFSCs markers and stem cell pluripotency associated factors were all significantly decreased; the expression of Wnt signaling pathway genes LEF1, TCF1,c-Myc were significantly changed. These results suggested that Sox9 plays important role in gHFSCs characteristics and pluripotency maintenance.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu Ddoi
10.1007/s10616-018-0206-8subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1155-1165issue
4eissn
0920-9069issn
1573-0778pii
10.1007/s10616-018-0206-8journal_volume
70pub_type
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