A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein-protein interactions mediated by G protein-coupled receptors.

abstract：:The use of the gadd153promoter to induce expression of a reporter geneunder heat stress conditions was investigated,since the results of previous studies have suggestedthat the gadd153promoter is likely to be activated by the indirecteffects of hyperthermia, that is, by DNA damage thatoccurs when reactive oxygen speci...

journal_title：Cytotechnology

authors： Bouhon IA,Ito A,Shinkai M,Honda H,Kobayashi T

更新日期：2000-07-01 00:00:00

abstract：:Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology an...

journal_title：Cytotechnology

authors： Cheng S,Yang X,Zhang Y,Xiao C

更新日期：2013-08-01 00:00:00

abstract：:IL-5 plays important roles in eosinophil differentiation, expansion, and recruitment. The regulation of IL-5 seems critical for the treatment of eosinophil-mediated allergic reactions. However, the precise mechanisms for IL-5 regulation remain unknown. In this study, we investigated how IL-5 production is regulated. T...

journal_title：Cytotechnology

authors： Hashiguchi M,Kobayashi A,Kashiwakura Y,Kojima H,Kanno Y,Kurosu A,Tokudome S,Kobata T

更新日期：2014-05-01 00:00:00

abstract：:Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...

journal_title：Cytotechnology

authors： Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

更新日期：2018-06-01 00:00:00

abstract：:We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...

journal_title：Cytotechnology

authors： Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

更新日期：2001-07-01 00:00:00

abstract：:This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...

journal_title：Cytotechnology

authors： Kuhlmann I

更新日期：1995-06-01 00:00:00

abstract：:Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...

journal_title：Cytotechnology

authors： Heike Y,Tsuruo T

更新日期：1993-01-01 00:00:00

abstract：:The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...

journal_title：Cytotechnology

authors： Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

更新日期：2003-07-01 00:00:00

abstract：:Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...

journal_title：Cytotechnology

authors： Pamboukian MM,Jorge SA,Santos MG,Yokomizo AY,Pereira CA,Tonso A

更新日期：2008-05-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...

journal_title：Cytotechnology

authors： Kelly D,Campbell WJ,Tiesman J,Rizzino A

更新日期：1990-11-01 00:00:00

abstract：:TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...

journal_title：Cytotechnology

authors： Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

更新日期：2009-07-01 00:00:00

abstract：:Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...

journal_title：Cytotechnology

authors： Dufresne M,Warocquier-Clérout R

更新日期：2001-09-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...

journal_title：Cytotechnology

authors： Bayramoglu A,Urhan Kucuk M,Guler HI,Abaci O,Kucukkaya Y,Colak E

更新日期：2015-01-01 00:00:00

abstract：:Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...

journal_title：Cytotechnology

authors： Weng Z,Jin J,Shao C,Li H

更新日期：2020-04-01 00:00:00

abstract：:A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...

journal_title：Cytotechnology

authors： Inoue Y,Tsukamoto Y,Yamanaka M,Nakamura S,Inoue A,Nishino N,Kawahara H

更新日期：2010-08-01 00:00:00

abstract：:We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...

journal_title：Cytotechnology

authors： Akimaru K,Kuo MT,Furuta K,Suzuki M,Noyori R,Ishikawa T

更新日期：1996-01-01 00:00:00

abstract：:Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...

journal_title：Cytotechnology

authors： Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

更新日期：2021-02-01 00:00:00

abstract：:MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

journal_title：Cytotechnology

authors： Tan PY,Wen LJ,Li HN,Chai SW

更新日期：2020-10-01 00:00:00

abstract：:A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...

journal_title：Cytotechnology

authors： Lee HJ,Kang HY,Kim CH,Kim HS,Kwon MC,Kim SM,Shin IS,Lee HY

更新日期：2006-11-01 00:00:00

abstract：:Low-frequency dielectric spectroscopy has been used in situ, i.e. while the cells are still attached to their microsupport, to monitor the changes of biomass accompanying the growth of anchorage-dependent cells. This method, when compared to Aperture Impedance Pulse Spectroscopy (also called electronic sizing), is cha...

journal_title：Cytotechnology

authors： Degouys V,Cerckel I,Garcia A,Harfield J,Dubois D,Fabry L,Miller AO

更新日期：1993-01-01 00:00:00

abstract：:A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...

journal_title：Cytotechnology

authors： Kobayashi J,Imanishi S,Inoue H,Ohsuye K,Yamaichi K,Tsuruoka N,Tanaka S

更新日期：1992-01-01 00:00:00

abstract：:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...

journal_title：Cytotechnology

authors： Zecchini TA,Smith RJ

更新日期：1999-07-01 00:00:00

abstract：:We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...

journal_title：Cytotechnology

authors： Xu Z,Yu L,Lu H,Feng W,Chen L,Zhou J,Yang X,Qi Z

更新日期：2018-12-01 00:00:00

abstract：:There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

journal_title：Cytotechnology

authors： Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

更新日期：2021-02-01 00:00:00

abstract：CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...

journal_title：Cytotechnology

authors： Betenbaugh MJ,Ailor E,Whiteley E,Hinderliter P,Hsu TA

更新日期：1996-01-01 00:00:00

abstract：:We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...

journal_title：Cytotechnology

authors： Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

更新日期：2006-05-01 00:00:00

abstract：:Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...

journal_title：Cytotechnology

authors： Martínez-Sales V,Vila V,Ferrando M,Reganon E

更新日期：2011-01-01 00:00:00

abstract：:Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...

journal_title：Cytotechnology

authors： Ila HB,Topaktas M,Arslan M,Büyükleyla M

更新日期：2014-08-01 00:00:00