Silver/silver chloride nanoparticles inhibit the proliferation of human glioblastoma cells.

abstract：:Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...

journal_title：Cytotechnology

authors： Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

更新日期：2019-02-01 00:00:00

abstract：:Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...

journal_title：Cytotechnology

authors： Rahman MS,Khan F,Syeda PK,Nishimura K,Jisaka M,Nagaya T,Shono F,Yokota K

更新日期：2014-08-01 00:00:00

abstract：:We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...

journal_title：Cytotechnology

authors： Rodas VM,Marques FH,Honda MT,Soares DM,Jorge SA,Antoniazzi MM,Medugno C,Castro ME,Ribeiro BM,Souza ML,Tonso A,Pereira CA

更新日期：2005-06-01 00:00:00

abstract：:Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without a...

journal_title：Cytotechnology

authors： Yagasaki K,Miura Y,Okauchi R,Furuse T

更新日期：2000-07-01 00:00:00

abstract：:Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...

journal_title：Cytotechnology

authors： Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

更新日期：1997-01-01 00:00:00

abstract：:Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...

journal_title：Cytotechnology

authors： Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

更新日期：2008-09-01 00:00:00

abstract：:A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...

journal_title：Cytotechnology

authors： Inoue Y,Tsukamoto Y,Yamanaka M,Nakamura S,Inoue A,Nishino N,Kawahara H

更新日期：2010-08-01 00:00:00

abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...

journal_title：Cytotechnology

authors： Shoyama Y,Tanaka H,Fukuda N

更新日期：1999-09-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...

journal_title：Cytotechnology

authors： Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

更新日期：2018-12-01 00:00:00

abstract：:The Chinook salmon embryo cell line, CHSE-214, grew well in fetal bovine serum (FBS) but poorly in dialyzed (d) FBS. Purines restored most but not all growth-promoting activity to dFBS, which suggests that purines account for a large portion of the dialyzable fraction's growth-promoting activity. CHSE-214 died in newb...

journal_title：Cytotechnology

authors： Ganassin RC,Bols NC

更新日期：1992-01-01 00:00:00

abstract：:This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...

journal_title：Cytotechnology

authors： Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

更新日期：2014-08-01 00:00:00

abstract：:Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...

journal_title：Cytotechnology

authors： Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

更新日期：2008-03-01 00:00:00

abstract：:Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...

journal_title：Cytotechnology

authors： Chou CC

更新日期：2014-10-01 00:00:00

abstract：:The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...

journal_title：Cytotechnology

authors： Barnabé N,Butler M

更新日期：2000-10-01 00:00:00

abstract：:"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...

journal_title：Cytotechnology

authors： Dudek D

更新日期：1994-01-01 00:00:00

abstract：:Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...

journal_title：Cytotechnology

authors： Marques CL,Rafael MS,Cancela ML,Laizé V

更新日期：2007-09-01 00:00:00

abstract：:A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...

journal_title：Cytotechnology

authors： Horikawa I,Michishita E,Barrett JC

更新日期：2004-06-01 00:00:00

abstract：:Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...

journal_title：Cytotechnology

authors： Sahraneshin Samani F,Moore JK,Khosravani P,Ebrahimi M

更新日期：2014-08-01 00:00:00

abstract：:In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressure by reducing the ...

journal_title：Cytotechnology

authors： Teruya K,Zhang Y,Katakura Y,Shirahata S

更新日期：2002-11-01 00:00:00

abstract：:NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed ...

journal_title：Cytotechnology

authors： Keen MJ,Steward TW

更新日期：1995-10-01 00:00:00

abstract：:The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...

journal_title：Cytotechnology

authors： Wikarta JM,Kim SM

更新日期：2016-08-01 00:00:00

abstract：:Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...

journal_title：Cytotechnology

authors： Schrimpf G,Schröder M,Weitnauer E,Friedl P

更新日期：1994-01-01 00:00:00

abstract：:Infection of human B cells with Epstein-Barr virus (EBV) induces polyclonal activation in almost all infected cells, but a small proportion of infected cells are transformed to immortalized lymphoblastoid cell lines. Since B cells are activated also by CD40 ligand (CD40L) and Toll-like receptor (TLR) agonists via a si...

journal_title：Cytotechnology

authors： Younesi V,Shirazi FG,Memarian A,Amanzadeh A,Jeddi-Tehrani M,Shokri F

更新日期：2014-01-01 00:00:00

abstract：:With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...

journal_title：Cytotechnology

authors： Guan YH,Kemp RB

更新日期：1999-07-01 00:00:00

abstract：:Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium ...

journal_title：Cytotechnology

authors： Gammon MC,Banas MH,Boccumini LE,Sigal NH,Zweerink HJ

更新日期：1990-01-01 00:00:00

abstract：:A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...

journal_title：Cytotechnology

authors： Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

更新日期：1994-01-01 00:00:00

abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...

journal_title：Cytotechnology

authors： Feng W,Liang C,Wang C,Yu X,Li Q,Yang H

更新日期：2018-10-01 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...

journal_title：Cytotechnology

authors： Keenan J,Pearson D,Clynes M

更新日期：2006-03-01 00:00:00

abstract：:Taking into account that oxidative stress is among the factors causing cancer-related death; chemoprevention which consists in using antioxidant substances such as phenolics could prevent cancer formation and progression. In the present study, phenolic contents and antioxidant activities of methanolic extracts from th...

journal_title：Cytotechnology

authors： Boulaaba M,Tsolmon S,Ksouri R,Han J,Kawada K,Smaoui A,Abdelly C,Isoda H

更新日期：2013-12-01 00:00:00