Abstract：
:Actions of chlorogenic acid, a major component of coffee, andits constituents, caffeic and quinic acids, on theproliferation and invasion of AH109A, a rat ascites hepatomacell line, were investigated using in vitro assay systems. Allthree components suppressed the AH109A invasion atconcentrations of 5-40 muM without altering the cellproliferation. At the concentration of 10 muM, chlorogenic,caffeic and quinic acids significantly (P < 0.05) suppressedthe invasion by 68%, 36% and 31%, respectively, implying thatthe suppressive effect of chlorogenic acid on the AH109Ainvasion might result from the additive effects of itsconstituents, caffeic and quinic acids. At the concentrationof 10 muM, cinnamic acid and p-coumaric acid (4-hydroxycinnamicacid) exerted no or little influence on the invasion, whereascaffeic acid (3,4-dihydroxycinnamic acid) significantly (P <0.05) suppressed it, suggesting the possible involvement ofthe 3,4-dihydroxy group of caffeic acid in the suppression.Chlorogenic acid was thus demonstrated to be one of thechemical entities in coffee suppressing the hepatoma invasionin vitro, and both of its constituents, caffeic and quinicacids, to be responsible for the anti-invasive activity. Theseresults suggest the existence of nutritionally andpharmacologically important substances in coffee which controltumor cell invasion.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Yagasaki K,Miura Y,Okauchi R,Furuse Tdoi
10.1023/A:1008141918852subject
Has Abstractpub_date
2000-07-01 00:00:00pages
229-35issue
1-3eissn
0920-9069issn
1573-0778journal_volume
33pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract：:MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-020-00418-3
更新日期：2020-10-01 00:00:00
abstract：:Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the character...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-015-9869-6
更新日期：2016-08-01 00:00:00
abstract：:A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-004-5122-4
更新日期：2004-06-01 00:00:00
abstract：:A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00365093
更新日期：1990-09-01 00:00:00
abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The ai...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-011-9386-1
更新日期：2012-01-01 00:00:00
abstract：:Hepatic stellate cells (HSCs) are key players in liver fibrosis and regeneration via collagen degradation and synthesis. These phenomena involve inflammatory cytokines released from non-parenchymal liver cells such as Kupffer cells. Although the effects of individual cytokines on many cell types have been investigated...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-016-9948-3
更新日期：2017-06-01 00:00:00
abstract：:We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF02521744
更新日期：1992-01-01 00:00:00
abstract：:Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9617-8
更新日期：2014-08-01 00:00:00
abstract：:Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This st...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-016-9970-5
更新日期：2016-12-01 00:00:00
abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9536-8
更新日期：2014-01-01 00:00:00
abstract：:Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0253-1
更新日期：2018-12-01 00:00:00
abstract：:Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9609-8
更新日期：2014-08-01 00:00:00
abstract：:The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0081-8
更新日期：2017-10-01 00:00:00
abstract：:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0206-8
更新日期：2018-08-01 00:00:00
abstract：:In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9715-2
更新日期：2015-10-01 00:00:00
abstract：:There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1021106116887
更新日期：2002-01-01 00:00:00
abstract：:NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00749658
更新日期：1995-10-01 00:00:00
abstract：:The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00736814
更新日期：1991-02-01 00:00:00
abstract：:Structure-activity relationship of antioxidants for the protective effects on linoleic acid hydroperoxide (LOOH)-induced toxicity were examined in cultured human umbilical vein endothelial cells. alpha-Tocopherol, 2,2,5,7,8-pentamethylchroman-6-ol, butylated hydroxytoluene, probucol, and fatty acid esters of ascorbic ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008139412588
更新日期：2001-01-01 00:00:00
abstract：:Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-007-9082-3
更新日期：2007-06-01 00:00:00
abstract：:The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Although a variety of assays have been used to examine T cell responses in vitro, standardized ex vivo detection of antigen-specific CD4+ T cells from human circulatory PBMCs remains constrained by low-dimensional characterization outputs and the need for polyclonal, mitogen-induced expansion methods to generate detec...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0222-8
更新日期：2018-10-01 00:00:00
abstract：:Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-004-7996-6
更新日期：2004-07-01 00:00:00
abstract：:We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1014060113186
更新日期：2001-07-01 00:00:00
abstract：:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00148808
更新日期：1990-07-01 00:00:00
abstract：:Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-016-0003-1
更新日期：2016-10-01 00:00:00
abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0173-5
更新日期：2018-04-01 00:00:00
abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0221-9
更新日期：2018-10-01 00:00:00
abstract：:Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9637-4
更新日期：2014-10-01 00:00:00
abstract：:Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-015-9857-x
更新日期：2016-08-01 00:00:00
