Abstract：
:In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate into mature adipocytes by the addition of a cocktail containing dexamethasone, insulin, 3-isobutyl-1-methylxanthine, and oleic acid for 8 days. Successful differentiation was demonstrated by the development of lipid droplets and the expression of key marker genes including peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding protein-α (CEBP/α) and adipocyte fatty acid-binding protein (FABP4). Our results showed that duck primary preadipocytes began to adhere 12 h after seeding as short spindle shapes or litter triangles, which grew quickly 3 days post attachment and maintained stable after day 7. After 8 days the preadipocytes were induced to differentiate into mature adipocytes, which were stained red by oil red O. Additionally, it showed that during preadipocyte differentiation PPARγ mRNA was highly expressed at day 3, while CEBP/α and FABP4 mRNA peaked at day 5 and 8, respectively. These results indicate that we have successfully isolated and cultured Peking duck preadipocytes and successfully induced them to differentiate into mature adipocytes. This work could lay a foundation for further research into waterfowl adipogenesis.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JWdoi
10.1007/s10616-014-9715-2subject
Has Abstractpub_date
2015-10-01 00:00:00pages
773-81issue
5eissn
0920-9069issn
1573-0778journal_volume
67pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract：:Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/b:cyto.0000039918.80472.0e
更新日期：2003-11-01 00:00:00
abstract：:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00148808
更新日期：1990-07-01 00:00:00
abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00556297
更新日期：1991-03-01 00:00:00
abstract：:A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00772193
更新日期：1994-01-01 00:00:00
abstract：:Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-020-00449-w
更新日期：2021-02-01 00:00:00
abstract：:Previous studies have demonstrated antidiabetic effects for rooibos (Aspalathus linearis) and aspalathin (ASP), one of its main polyphenols. Rooibos, an endemic plant of South Africa, is well-known for its use as herbal tea. Green ('unfermented') rooibos has been shown to contain more ASP than 'fermented' rooibos tea,...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9816-y
更新日期：2015-08-01 00:00:00
abstract：:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0206-8
更新日期：2018-08-01 00:00:00
abstract：:The aim of the study was to obtain the highest number of multipotent adipose-derived mesenchymal stem cells (ADMSCs) by using culture conditions which favour cell expansion without loss of mesenchymal stem cells (MSC)-like properties. Based on the assumption that stem cells reside in niches characterized by hypoxic co...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9731-2
更新日期：2015-12-01 00:00:00
abstract：:In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-008-9127-2
更新日期：2008-06-01 00:00:00
abstract：:A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1023945218123
更新日期：2002-09-01 00:00:00
abstract：:The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-005-4637-7
更新日期：2005-09-01 00:00:00
abstract：:There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1021106116887
更新日期：2002-01-01 00:00:00
abstract：:The cAMP and Ca(2+) signaling pathways activate the transcription factor CREB through its phosphorylation at Serine 133. Activation of CREB is involved in the regulation of various biological phenomena. To understand further the mechanisms of the regulation of CREB activity in response to activation of the cAMP and Ca...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-005-3763-6
更新日期：2005-01-01 00:00:00
abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0116-1
更新日期：2018-06-01 00:00:00
abstract：:The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1022435829894
更新日期：2002-05-01 00:00:00
abstract：:Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00353702
更新日期：1991-05-01 00:00:00
abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0173-5
更新日期：2018-04-01 00:00:00
abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0221-9
更新日期：2018-10-01 00:00:00
abstract：:The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008068718392
更新日期：1999-03-01 00:00:00
abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-006-9002-y
更新日期：2006-03-01 00:00:00
abstract：:In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-020-00419-2
更新日期：2020-10-01 00:00:00
abstract：:A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF02525492
更新日期：1992-01-01 00:00:00
abstract：:Lymphoid cells expressing sufficient levels of Bcl-2 or E1B-19K are known to resist to induction of apoptosis in glutamine-free or nutrient-limited batch cultures. However, despite the increased viability and prolonged stationary phase achieved in batch culture, product yields are not necessarily improved. Here we hav...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008054403470
更新日期：1998-11-01 00:00:00
abstract：:Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-016-0003-1
更新日期：2016-10-01 00:00:00
abstract：:The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9704-5
更新日期：2015-05-01 00:00:00
abstract：:Tea catechin is one of the compounds that are closely related to obesity and insulin sensitivity. In order to determine the effect of catechin on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different kinds of catechins. Our results showed that catechins, especially epigallocatechin gallate (EGCG), ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-010-9285-x
更新日期：2010-07-01 00:00:00
abstract：:Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0242-4
更新日期：2018-12-01 00:00:00
abstract：:Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1007928203997
更新日期：1997-11-01 00:00:00
abstract：:Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-020-00375-x
更新日期：2020-04-01 00:00:00
abstract：:Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00133241
更新日期：1989-08-01 00:00:00
