Abstract:
:A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cell-laden gel occurred subsequently to create a new zone in the cell chamber. In such a bioreactor cells are retained in the reactor, the high molecular product(s) accumulate in the cell chamber, while the small molecular weight nutrients and metabolites are replenished and removed from the medium chamber. By adjusting the flow rates for cell and medium chambers, the resident time for cells, high and low molecular weight components of the system can be manipulated separately. The new bioreactor, in both flat-bed and hollow-fiber configurations, was used to cultivate recombinant human cell, 293, for Protein C production over 60 to 90 days.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Scholz M,Hu WSdoi
10.1007/BF00365093subject
Has Abstractpub_date
1990-09-01 00:00:00pages
127-37issue
2eissn
0920-9069issn
1573-0778journal_volume
4pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008038515285
更新日期:1999-07-01 00:00:00
abstract::The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007945826228
更新日期:1997-07-01 00:00:00
abstract::A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. C...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007924119018
更新日期:1997-11-01 00:00:00
abstract::High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353919
更新日期:1996-01-01 00:00:00
abstract::More than 35 years ago double Nobel laureate Linus Carl Pauling published a powerful model of the molecular mechanism of general anesthesia, generally referred to as the hydrate-microcrystal (aqueous-phase) theory. This hypothesis, based on the molecular behavior of water molecules, did not receive serious attention d...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9216-x
更新日期:2009-04-01 00:00:00
abstract::The cAMP and Ca(2+) signaling pathways activate the transcription factor CREB through its phosphorylation at Serine 133. Activation of CREB is involved in the regulation of various biological phenomena. To understand further the mechanisms of the regulation of CREB activity in response to activation of the cAMP and Ca...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3763-6
更新日期:2005-01-01 00:00:00
abstract::Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9811-3
更新日期:2016-08-01 00:00:00
abstract::Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008187310534
更新日期:2000-11-01 00:00:00
abstract::We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00563783
更新日期:1990-11-01 00:00:00
abstract::Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00761778
更新日期:1994-01-01 00:00:00
abstract::Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-011-9390-5
更新日期:2012-01-01 00:00:00
abstract::Chemical genomics is a new research paradigm with importantapplications in drug discovery. It links genomic targets withsmall-molecule chemistries thereby allowing for efficient targetvalidation and lead compound identification. ACADIA'schemical-genomics platform consists of a large and diverse small-moleculelibrary (...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1021169023731
更新日期:2002-01-01 00:00:00
abstract::The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9583-1
更新日期:2013-10-01 00:00:00
abstract::A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-005-3751-x
更新日期:2005-01-01 00:00:00
abstract::Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0127-y
更新日期:2018-02-01 00:00:00
abstract::The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of act...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9814-0
更新日期:2016-08-01 00:00:00
abstract::Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00386140
更新日期:1989-05-01 00:00:00
abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9835-8
更新日期:2016-08-01 00:00:00
abstract::A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365489
更新日期:1990-05-01 00:00:00
abstract::There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0067-6
更新日期:2017-04-01 00:00:00
abstract::We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744216
更新日期:1996-01-01 00:00:00
abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10616-018-0225-5
更新日期:2018-10-01 00:00:00
abstract::A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, an...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02215660
更新日期:1996-06-01 00:00:00
abstract::Fusion of lymphoblastoid cell lines that produce human monoclonal antibodies against Pseudomonas aeruginosa with the human/mouse heteromyeloma SHM-D33 generated heterohybrids that were stable and secreted antibody in the range of 20 to 300 micrograms/ml. One of the hybridoma cell lines ws adapted to serum-free medium ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365266
更新日期:1990-01-01 00:00:00
abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024231023015
更新日期:2003-01-01 00:00:00
abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9191-2
更新日期:2009-01-01 00:00:00
abstract::Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW co...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365080
更新日期:1988-11-01 00:00:00
abstract::The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736814
更新日期:1991-02-01 00:00:00
abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9102-3
更新日期:2008-01-01 00:00:00
abstract::A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal ant...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9243-7
更新日期:2010-08-01 00:00:00