DNA and cell death.

abstract：:In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to ree...

journal_title：Cytotechnology

authors： Fernyhough ME,Vierck JL,Hausman GJ,Mir PS,Okine EK,Dodson MV

更新日期：2004-10-01 00:00:00

abstract：:Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...

journal_title：Cytotechnology

authors： Chen J,Ran Y,Hong C,Chen Z,You Y

更新日期：2010-10-01 00:00:00

abstract：:A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...

journal_title：Cytotechnology

authors： Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

更新日期：1994-01-01 00:00:00

abstract：:A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...

journal_title：Cytotechnology

authors： Matsuda C,Takagi M,Hattori T,Wakitani S,Yoshida T

更新日期：2005-01-01 00:00:00

abstract：:Sorbaria kirilowii (Regel) Maxim, a plant found in China, Korea, Japan, and east of Europe, is a common herb used for traditional medicinal purposes. However, its ability to prevent photoaging has not been studied. In this study, we investigated the anti-photoaging functions of an ethanol extract (Sk-EE) of S. kirilow...

journal_title：Cytotechnology

authors： Chen H,Jang J,Kopalli SR,Yum J,Yoon K,Cho JY

更新日期：2021-02-01 00:00:00

abstract：:Galangin, an active flavonoid present at high concentration in Alpinia officinarum Hance and propolis, shows cytotoxicity towards several cancer cell lines, including melanoma. However, the specific cellular targets of galangin-induced cytotoxicity in melanoma are still unknown. Here, we investigated the effects of ga...

journal_title：Cytotechnology

authors： Zhang W,Lan Y,Huang Q,Hua Z

更新日期：2013-05-01 00:00:00

abstract：:The aim of this study was to reveal the biological activities and in vivo toxicity profiles of n-hexane, chloroform and methanol extracts of brown algae Halopteris scoparia L. Sauvageau. In this study, extracts were tested for their phytochemical contents and antioxidant activities. The cytotoxic activities of the ext...

journal_title：Cytotechnology

authors： Güner A,Nalbantsoy A,Sukatar A,Karabay Yavaşoğlu NÜ

更新日期：2019-06-01 00:00:00

abstract：:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...

journal_title：Cytotechnology

authors： Nielsen LK,Smyth GK,Greenfield PF

更新日期：1992-01-01 00:00:00

abstract：:Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...

journal_title：Cytotechnology

authors： Damia G,D'Incalci M

更新日期：1998-09-01 00:00:00

abstract：:Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...

journal_title：Cytotechnology

authors： Yamashoji S,Isshiki K

更新日期：2001-11-01 00:00:00

abstract：:Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...

journal_title：Cytotechnology

authors： Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

更新日期：2020-06-01 00:00:00

abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...

journal_title：Cytotechnology

authors： Shoyama Y,Tanaka H,Fukuda N

更新日期：1999-09-01 00:00:00

abstract：:Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...

journal_title：Cytotechnology

authors： Eren Y,Erdoğmuş SF,Akyıl D,Özkara A

更新日期：2016-08-01 00:00:00

abstract：:Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...

journal_title：Cytotechnology

authors： Adams AM,Soames JV,Searle RF

更新日期：1988-02-01 00:00:00

abstract：:The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...

journal_title：Cytotechnology

authors： Amadori M,Berneri C

更新日期：1993-01-01 00:00:00

abstract：:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...

journal_title：Cytotechnology

authors： Lim HS,Chang KH,Kim JH

更新日期：1999-11-01 00:00:00

abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...

journal_title：Cytotechnology

authors： Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

更新日期：2014-01-01 00:00:00

abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...

journal_title：Cytotechnology

authors： Ribeiro DL,Cilião HL,Specian AFL,Serpeloni JM,De Oliveira MT,Varanda EA,Vilegas W,Saldanha LL,Martínez-López W,Dokkedal AL,Cólus IMS

更新日期：2018-04-01 00:00:00

abstract：:Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This st...

journal_title：Cytotechnology

authors： Yokoi S,Murakami M,Morikawa M,Goi T,Yamaguchi A,Terada S

更新日期：2016-12-01 00:00:00

abstract：:Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...

journal_title：Cytotechnology

authors： Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

更新日期：2018-10-01 00:00:00

abstract：:Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

journal_title：Cytotechnology

authors： Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

更新日期：2019-07-12 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...

journal_title：Cytotechnology

authors： Wirth M,Berthold E,Grashoff M,Pfützner H,Schubert U,Hauser H

更新日期：1994-01-01 00:00:00

abstract：:Matrine is a bioactive component of the traditional Chinese medical herb Sophora flavescens that has been used in China to treat various kinds of diseases including virus hepatitis. However, the molecular mechanisms underlying its hepatoprotective effects remains elusive. In the present study, primary human hepatocyte...

journal_title：Cytotechnology

authors： Gong X,Gao Y,Guo G,Vondran FW,Schwartlander R,Efimova E,Pless G,Sauera IM,Neuhaus P

更新日期：2015-03-01 00:00:00

abstract：:Cisplatin is one of the most effective chemotherapeutic agents but injury may occur at higher doses. The aim of this study was to investigate the effect of bilberry on cisplatin induced toxic effects in rat ovary. Twenty-one female Wistar-Albino rats were utilized to form three groups: In group 1 (control group), each...

journal_title：Cytotechnology

authors： Pandir D,Kara O,Kara M

更新日期：2014-08-01 00:00:00

abstract：:Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...

journal_title：Cytotechnology

authors： Weng Z,Jin J,Shao C,Li H

更新日期：2020-04-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...

journal_title：Cytotechnology

authors： Keenan J,Pearson D,Clynes M

更新日期：2006-03-01 00:00:00

abstract：:Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...

journal_title：Cytotechnology

authors： Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

更新日期：2013-05-01 00:00:00

abstract：:Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...

journal_title：Cytotechnology

authors： Turkez H,Aydin E,Aslan A

更新日期：2012-12-01 00:00:00

abstract：:The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...

journal_title：Cytotechnology

authors： Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

更新日期：2015-05-01 00:00:00