Electroporation: an arsenal of application.


:Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed, whose most important point is that it adopts low energy and rectangular pulse that could obtain high transfection efficiency and low damage to the embryo. This paper reviewed on the pool of application: from lab works to human clinical treatments.






Yuan TF




Has Abstract


2007-06-01 00:00:00












  • Optimization of macrophage isolation from the Persian sturgeon and the Caspian kutum fish: a comparative study.

    abstract::The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...


    pub_type: 杂志文章


    authors: Rastgar S,Movahedinia A,Salamat N,Salati A,Zabihi E

    更新日期:2018-12-01 00:00:00

  • MicroRNAs: recently discovered key regulators of proliferation and apoptosis in animal cells : Identification of miRNAs regulating growth and survival.

    abstract::The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...


    pub_type: 杂志文章


    authors: Gammell P

    更新日期:2007-04-01 00:00:00

  • A methoxyflavanone derivative from the Asian medicinal herb (Perilla frutescens) induces p53-mediated G2/M cell cycle arrest and apoptosis in A549 human lung adenocarcinoma.

    abstract::Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...


    pub_type: 杂志文章


    authors: Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

    更新日期:2018-06-01 00:00:00

  • A continuous multistage roller reactor for animal cell culture: 1. Patterns of growth, production and catabolism of a murine hybridoma.

    abstract::A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...


    pub_type: 杂志文章


    authors: Borth N,Steindl F,Weigang F,Reiter M,Katinger H

    更新日期:1990-05-01 00:00:00

  • Zoledronic acid suppresses metastasis of esophageal squamous cell carcinoma cells through upregulating the tight junction protein occludin.

    abstract::We have previously demonstrated the radio-sensitizing effect of zoledronic acid (ZOL), a third generation bisphosphonate, on human esophageal squamous cell carcinoma (ESCC) cells. Here we show that ZOL suppresses metastatic progression of ESCC cells mainly through up-regulating the tight junction protein occludin. Exp...


    pub_type: 杂志文章


    authors: Lin C,Xin S,Qin X,Li H,Lin L,You Y

    更新日期:2016-08-01 00:00:00

  • Chromatid segregation analysis in native human lymphocyte anaphases using sequential fluorescence in situ hybridization.

    abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...


    pub_type: 杂志文章


    authors: Escudero T,Coll MD,Fuster C,Egozcue J

    更新日期:2002-09-01 00:00:00

  • Production of immunoglobulin A in different reactor configurations.

    abstract::A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...


    pub_type: 杂志文章


    authors: Stoll T,Perregaux C,von Stockar U,Marison IW

    更新日期:1995-02-01 00:00:00

  • Cloning of a new fibroblast cell line from an early primary culture from mandarin fish (Siniperca chuatsi) fry for efficient proliferation of megalocytiviruses.

    abstract::Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...


    pub_type: 杂志文章


    authors: Dong C,Shuang F,Weng S,He J

    更新日期:2014-12-01 00:00:00

  • Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study.

    abstract::Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...


    pub_type: 杂志文章


    authors: Sen S,Srienc F,Hu WS

    更新日期:1989-05-01 00:00:00

  • Identification of the genes specifically expressed in orally tolerized T cells.

    abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...


    pub_type: 杂志文章


    authors: Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

    更新日期:2003-11-01 00:00:00

  • Septin 6 localizes to microtubules in neuronal dendrites.

    abstract::In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...


    pub_type: 杂志文章


    authors: Moon IS,Lee H,Walikonis RS

    更新日期:2013-03-01 00:00:00

  • Are we prepared for animal cell technology in the 21st century?

    abstract::Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...


    pub_type: 杂志文章


    authors: Cooney CL

    更新日期:1995-01-01 00:00:00

  • Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.

    abstract::In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...


    pub_type: 杂志文章


    authors: Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

    更新日期:1989-02-01 00:00:00

  • A direct computer control concept for mammalian cell fermentation processes.

    abstract::In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...


    pub_type: 杂志文章


    authors: Büntemeyer H,Marzahl R,Lehmann J

    更新日期:1994-01-01 00:00:00

  • Atorvastatin neutralises the thrombin-induced tissue factor expresion in endothelial cells via geranylgeranyl pyrophosphate.

    abstract::Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...


    pub_type: 杂志文章


    authors: Martínez-Sales V,Vila V,Ferrando M,Reganon E

    更新日期:2011-01-01 00:00:00

  • Evaluation of electroporation-induced adverse effects on adipose-derived stem cell exosomes.

    abstract::In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...


    pub_type: 杂志文章


    authors: Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux M

    更新日期:2016-10-01 00:00:00

  • Effect of matrine on primary human hepatocytes in vitro.

    abstract::Matrine is a bioactive component of the traditional Chinese medical herb Sophora flavescens that has been used in China to treat various kinds of diseases including virus hepatitis. However, the molecular mechanisms underlying its hepatoprotective effects remains elusive. In the present study, primary human hepatocyte...


    pub_type: 杂志文章


    authors: Gong X,Gao Y,Guo G,Vondran FW,Schwartlander R,Efimova E,Pless G,Sauera IM,Neuhaus P

    更新日期:2015-03-01 00:00:00

  • The effect of glucose and glutamine on the intracellular nucleotide pool and oxygen uptake rate of a murine hybridoma.

    abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...


    pub_type: 杂志文章


    authors: Barnabé N,Butler M

    更新日期:2000-10-01 00:00:00

  • Influence of aeration-homogenization system in stirred tank bioreactors, dissolved oxygen concentration and pH control mode on BHK-21 cell growth and metabolism.

    abstract::This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...


    pub_type: 杂志文章


    authors: Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

    更新日期:2014-08-01 00:00:00

  • New applications of alphavirus-based expression vectors.

    abstract::Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...


    pub_type: 杂志文章


    authors: Boorsma M,Koller D,Renner WA,Bachmann MF

    更新日期:2001-05-01 00:00:00

  • Sequential cultivation of human epidermal keratinocytes and dermal mesenchymal like stromal cells in vitro.

    abstract::Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...


    pub_type: 杂志文章


    authors: Mahabal S,Konala VB,Mamidi MK,Kanafi MM,Mishra S,Shankar K,Pal R,Bhonde R

    更新日期:2016-08-01 00:00:00

  • Erythropoietin processing in erythropoietic system and central nervous system.

    abstract::We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...


    pub_type: 杂志文章


    authors: Nagao M,Masuda S,Ueda M,Sasaki R

    更新日期:1995-01-01 00:00:00

  • Cell Culture Derived AgMNPV Bioinsecticide: Biological Constraints and Bioprocess Issues.

    abstract::We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...


    pub_type: 杂志文章


    authors: Rodas VM,Marques FH,Honda MT,Soares DM,Jorge SA,Antoniazzi MM,Medugno C,Castro ME,Ribeiro BM,Souza ML,Tonso A,Pereira CA

    更新日期:2005-06-01 00:00:00

  • Use of cell cultures, nuclear and radioanalytical techniques for metallotoxicological studies at the JRC-Ispra.

    abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...


    pub_type: 杂志文章


    authors: Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

    更新日期:1993-01-01 00:00:00

  • Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo.

    abstract::Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...


    pub_type: 杂志文章


    authors: Kumalasari ID,Harmayani E,Lestari LA,Raharjo S,Asmara W,Nishi K,Sugahara T

    更新日期:2012-03-01 00:00:00

  • Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.

    abstract::TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...


    pub_type: 杂志文章


    authors: Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

    更新日期:2009-07-01 00:00:00

  • Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

    abstract::We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...


    pub_type: 杂志文章


    authors: Thomou H,Sambani C,Kitsiou P,Politis G

    更新日期:1988-07-01 00:00:00

  • A speedy method to detect inserted DNA fragment in cell lines transfected with retroviral vectors.

    abstract::Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...


    pub_type: 杂志文章


    authors: Ding LH,Iimura E,Saijo K,Hamada H,Ohno T

    更新日期:2000-11-01 00:00:00

  • A practical approach for intracellular protein delivery.

    abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...


    pub_type: 杂志文章


    authors: Weill CO,Biri S,Adib A,Erbacher P

    更新日期:2008-01-01 00:00:00

  • Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture.

    abstract::The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...


    pub_type: 杂志文章


    authors: Wikarta JM,Kim SM

    更新日期:2016-08-01 00:00:00