Abstract:
:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybridoma cells to rIL-2, Tac gene was introduced by co-transfection with Tac gene expression plasmid pTB459 and G418 resistant gene expression plasmid pRSVneo. Tac cDNA transfected hybridoma (HBW-4.16.459-6-126) was induced to produce 6 times as much IgG by rIL-2 as was the control. This antibody production promoting phenomenon mediated by rIL-2 was depressed by anti-Tac antibody.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano Kdoi
10.1007/BF00148808subject
Has Abstractpub_date
1990-07-01 00:00:00pages
29-37issue
1eissn
0920-9069issn
1573-0778journal_volume
4pub_type
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