Abstract:
:NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed to grow in W38 medium and in a number of protein-free culture media which support the growth of other myeloma cell lines. NS0 cells are derived from the NS-1 cell line, which is known to require exogencus cholesterol. It was found that NS0 cells grew in W38 medium supplemented with phosphatidylcholine, cholesterol, and albumin and that NS0 were auxotrophic for cholesterol. Protein-free growth of NS0 cells was achieved by using β-cyclodextrin to replace albumin as a lipid carrier. The maximal cell density reached in this protein-free medium was in excess of 1.5×10(6) cell ml(-1). The lipid supplements in the medium precipitated after a few days storage at +4°C. In order to overcome this problem a protocol was developed which allowed NS0 cells to be adapted to cholesterol-independent growth in W38 medium. NS0.CF (cholesterol-independent NS0 cells) were cultured continuously in W38 medium for several months. In shake flask culture a cell density of 2.4×10(6) cells ml(-1) was achieved in W38 medium compared with 1.41×10(6) cells ml(-1) in RPMI 1640 medium containing 10% foetal bovine serum. NS0.CF cells readily grew in a 1 litre stirred bioreactor using W38 medium supplemented with Pluronic F68 reaching a density of 3.24×10(6) cells ml(-1). NS0.CF were cloned protein-free by limiting dilution in W38 medium, giving colonies in wells that were seeded at an average density of 0.32 cells per 200 μl. This study has demonstrated for the first time the growth of a cholesterol-requiring mouse myeloma cell line in a completely defined protein-free medium and its subsequent adaptation to cholesterol-independence.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Keen MJ,Steward TWdoi
10.1007/BF00749658subject
Has Abstractpub_date
1995-10-01 00:00:00pages
203-11issue
3eissn
0920-9069issn
1573-0778journal_volume
17pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00772193
更新日期:1994-01-01 00:00:00
abstract::Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0237-1
更新日期:2018-10-01 00:00:00
abstract::Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and genotoxic effects of this pesticide on human peripheral blood lymphocytes and Allium root meristematic cells were investigated by chromosomal aberrations (CAs) and Allium test. Human lymphocytes were treated with 62.5, 125, 2...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9741-0
更新日期:2015-12-01 00:00:00
abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008175226819
更新日期:2000-07-01 00:00:00
abstract::Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0253-1
更新日期:2018-12-01 00:00:00
abstract::Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0116-1
更新日期:2018-06-01 00:00:00
abstract::Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-011-9403-4
更新日期:2012-03-01 00:00:00
abstract::This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365341
更新日期:1996-01-01 00:00:00
abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00570891
更新日期:1992-01-01 00:00:00
abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008159912634
更新日期:2000-10-01 00:00:00
abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...
journal_title:Cytotechnology
pub_type: 杂志文章,评审
doi:10.1007/s10616-018-0225-5
更新日期:2018-10-01 00:00:00
abstract::Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1013192017048
更新日期:2001-05-01 00:00:00
abstract::A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365093
更新日期:1990-09-01 00:00:00
abstract::The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9087-y
更新日期:2007-07-01 00:00:00
abstract::We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00563783
更新日期:1990-11-01 00:00:00
abstract::Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749933
更新日期:1993-01-01 00:00:00
abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749004
更新日期:1993-01-01 00:00:00
abstract::The growth yields for glucose and glutamine of murine hybridoma cells entrapped in collagen gel particles were examined during the growth phase. The immobilized hybridoma cells were cultivated in a fluidized bed fermenter where the medium was circulating to supply oxygen separately. Procedures to supply an alkaline so...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00758178
更新日期:1994-01-01 00:00:00
abstract::As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-011-9387-0
更新日期:2011-12-01 00:00:00
abstract::Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0213-9
更新日期:2018-06-01 00:00:00
abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945218123
更新日期:2002-09-01 00:00:00
abstract::Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9609-8
更新日期:2014-08-01 00:00:00
abstract::We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02521744
更新日期:1992-01-01 00:00:00
abstract::A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749758
更新日期:1995-01-01 00:00:00
abstract::Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Hyperuricemia is an important risk factor for gout. Isorhamnetin (3'-O-methylquercetin) is an O-methylated flavonol, which occurs in onion, almond and sea buckthorn. It is also one of the metabolites of quercetin in mammals. In the present study, we investigated anti-hyperuricemic effect of isorhamnetin adopting both ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0275-8
更新日期:2019-02-01 00:00:00
abstract::Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-009-9193-0
更新日期:2009-01-01 00:00:00
abstract::Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0111-6
更新日期:2018-02-01 00:00:00
abstract::Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008187310534
更新日期:2000-11-01 00:00:00