Genotypic and phenotypic changes of BHK-21 cells grown in suspension cultures.

abstract：:Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This...

journal_title：Cytotechnology

authors： Mashima T,Seimiya H,Chen Z,Kataoka S,Tsuruo T

更新日期：1998-09-01 00:00:00

abstract：:To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...

journal_title：Cytotechnology

authors： Katakura Y,Seto P,Miura T,Ohashi H,Teruya K,Shirahata S

更新日期：1999-09-01 00:00:00

abstract：:Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...

journal_title：Cytotechnology

authors： Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

更新日期：2018-02-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00

abstract：:The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...

journal_title：Cytotechnology

authors： Zhou W,Rehm J,Europa A,Hu WS

更新日期：1997-07-01 00:00:00

abstract：:Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...

journal_title：Cytotechnology

authors： Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

更新日期：2019-02-01 00:00:00

abstract：:The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...

journal_title：Cytotechnology

authors： Vu NB,Nguyen TT,Tran LC,Do CD,Nguyen BH,Phan NK,Pham PV

更新日期：2013-08-01 00:00:00

abstract：:A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...

journal_title：Cytotechnology

authors： Horikawa I,Michishita E,Barrett JC

更新日期：2004-06-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in micros...

journal_title：Cytotechnology

authors： Zhang D,Tong A,Zhou L,Fang F,Guo G

更新日期：2012-12-01 00:00:00

abstract：:In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...

journal_title：Cytotechnology

authors： Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

更新日期：1989-02-01 00:00:00

abstract：:Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...

journal_title：Cytotechnology

authors： Han J,Talorete TP,Yamada P,Isoda H

更新日期：2009-01-01 00:00:00

abstract：:Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...

journal_title：Cytotechnology

authors： Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

更新日期：2003-11-01 00:00:00

abstract：:We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...

journal_title：Cytotechnology

authors： Nagao M,Masuda S,Ueda M,Sasaki R

更新日期：1995-01-01 00:00:00

abstract：:A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal ant...

journal_title：Cytotechnology

authors： Shinmoto H,Matsuo Y,Naganawa Y,Tomita S,Takano-Ishikawa Y

更新日期：2010-08-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...

journal_title：Cytotechnology

authors： Wirth M,Berthold E,Grashoff M,Pfützner H,Schubert U,Hauser H

更新日期：1994-01-01 00:00:00

abstract：:Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

journal_title：Cytotechnology

authors： Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

更新日期：2017-12-01 00:00:00

abstract：:Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...

journal_title：Cytotechnology

authors： Dufresne M,Warocquier-Clérout R

更新日期：2001-09-01 00:00:00

abstract：:Previously, it has been shown that the binding of epidermal growth factor (EGF) by a wide range of cells decreases as cell density increases. In this report, we demonstrate that KB cells treated chronically with phorbol esters continue to exhibit decreases in EGF receptor binding as cell density increases. This findin...

journal_title：Cytotechnology

authors： Rizzino A,Huebert C,Kuszynski C,Wilder PJ

更新日期：1991-10-01 00:00:00

abstract：:Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...

journal_title：Cytotechnology

authors： Macdonald RG

更新日期：1989-12-01 00:00:00

abstract：:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...

journal_title：Cytotechnology

authors： Lim HS,Chang KH,Kim JH

更新日期：1999-11-01 00:00:00

abstract：:We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...

journal_title：Cytotechnology

authors： Kelly D,Campbell WJ,Tiesman J,Rizzino A

更新日期：1990-11-01 00:00:00

abstract：:The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...

journal_title：Cytotechnology

authors： Ikonomou L,Bastin G,Schneider YJ,Agathos SN

更新日期：2004-01-01 00:00:00

abstract：:Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...

journal_title：Cytotechnology

authors： Cooney CL

更新日期：1995-01-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs), one type of mesenchymal stem cells, are considered to be a type of tool cells for regenerative medicine and tissue engineering. Our previous studies found that the stimulation with lipopolysaccharide (LPS) might introduce senescence of DPSCs, and this senescence would have a positive co...

journal_title：Cytotechnology

authors： Feng G,Zheng K,Cao T,Zhang J,Lian M,Huang D,Wei C,Gu Z,Feng X

更新日期：2018-06-01 00:00:00

abstract：:A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...

journal_title：Cytotechnology

authors： Dunstan CA,Andersson J,Bohlin L,Cox PA,Grönvik KO

更新日期：1994-01-01 00:00:00

abstract：:The CHO cell line has achieved considerable commercial importance as a vehicle for the production of human therapeutic proteins, but is known to lack a functional copy of the gene coding for α2,6-sialyltransferase (EC 2.4.99.1). The cDNA for rat α2,6-ST was expressed in a recombinant CHO cell line making interferon-γ,...

journal_title：Cytotechnology

authors： Monaco L,Marc A,Eon-Duval A,Acerbis G,Distefano G,Lamotte D,Engasser JM,Soria M,Jenkins N

更新日期：1996-01-01 00:00:00

abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...

journal_title：Cytotechnology

authors： Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

更新日期：1991-03-01 00:00:00

abstract：:Lymphoid cells expressing sufficient levels of Bcl-2 or E1B-19K are known to resist to induction of apoptosis in glutamine-free or nutrient-limited batch cultures. However, despite the increased viability and prolonged stationary phase achieved in batch culture, product yields are not necessarily improved. Here we hav...

journal_title：Cytotechnology

authors： Mercille S,Massie B

更新日期：1998-11-01 00:00:00

abstract：:Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...

journal_title：Cytotechnology

authors： Peterka M,Mandys V,Peterková R

更新日期：1991-09-01 00:00:00