Apoptosis resistance in tumor cells.


:Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This article describes the recent findings concerning the mechanisms of chemotherapy-induced apoptosis and discusses the implication of apoptosis resistance in cancer chemotherapy.






Mashima T,Seimiya H,Chen Z,Kataoka S,Tsuruo T




Has Abstract


1998-09-01 00:00:00












  • Structure and expression of integrated hepatitis B virus genes in an HBs antigen producing human cell line (huGK-14).

    abstract::A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. C...


    pub_type: 杂志文章


    authors: Nakamichi N,Noda A,Yonezu T,Koike K,Matsumura T

    更新日期:1997-11-01 00:00:00

  • Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line.

    abstract::The UFL-AG-286 cell line, established from embryonic tissue of the lepidopteran insect Anticarsia gemmatalis, has been identified as a good candidate to be used as a cellular substrate in the development of a process for in vitro production of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, a baculovirus w...


    pub_type: 杂志文章


    authors: Gioria VV,Jäger V,Claus JD

    更新日期:2006-10-01 00:00:00

  • Agitation and aeration effects in suspension mammalian cell cultures.

    abstract::Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...


    pub_type: 杂志文章


    authors: Marquis CP,Low KS,Barford JP,Harbour C

    更新日期:1989-08-01 00:00:00

  • Promoting effect of rapeseed proteins and peptides on Sf9 insect cell growth.

    abstract::The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...


    pub_type: 杂志文章


    authors: Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

    更新日期:2003-07-01 00:00:00

  • Cetyltriethylammonium bromide stimulating transcription of Bombyx mori nucleopolyhedrovirus gp64 gene promoter mediated by viral factors.

    abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...


    pub_type: 杂志文章


    authors: Zhou Y,Yi Y,Zhang Z,He J,Zhang Y

    更新日期:2003-01-01 00:00:00

  • Silencing of soluble epoxide hydrolase 2 gene reduces H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells via activating PI3K/Akt/GSK3β signaling pathway.

    abstract::Oxidative stress plays a vital role in the occurrence and development of intestinal injury. Soluble epoxide hydrolase 2 gene (EPHX2) is a class of hydrolytic enzymes. We aim to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells. IEC-6 cells ...


    pub_type: 杂志文章


    authors: Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

    更新日期:2020-02-01 00:00:00

  • Fibroblast growth factor 21 induces lipolysis more efficiently than it suppresses lipogenesis in goat adipocytes.

    abstract::Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...


    pub_type: 杂志文章


    authors: Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

    更新日期:2018-10-01 00:00:00

  • Localization of viral transforming sequences within marker chromosomes associated with tumor formation and progression in a murine fibrosarcoma.

    abstract::The low-metastatic RSV-transformed fibrosarcoma line B77-3T3 and its metastatic variant AA12, selected in vitro, have been analysed by blot and in situ hybridization with v-src and murine c-myc specific probes in order to detect molecular rearrangements underlying the transition from the low-metastatic to the high-met...


    pub_type: 杂志文章


    authors: Doneda L,Custode P,Morghen Cde G,Larizza L

    更新日期:1987-10-01 00:00:00

  • Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures.

    abstract::The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...


    pub_type: 杂志文章


    authors: Pichon D,Cudennec B,Huchette S,Djediat C,Renault T,Paillard C,Auzoux-Bordenave S

    更新日期:2013-10-01 00:00:00

  • Real architecture For 3D Tissue (RAFT™) culture system improves viability and maintains insulin and glucagon production of mouse pancreatic islet cells.

    abstract::There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D...


    pub_type: 杂志文章


    authors: Szebeni GJ,Tancos Z,Feher LZ,Alfoldi R,Kobolak J,Dinnyes A,Puskas LG

    更新日期:2017-04-01 00:00:00

  • A rat mammary gland cancer cell with stem cell properties of self-renewal and multi-lineage differentiation.

    abstract::The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...


    pub_type: 杂志文章


    authors: Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

    更新日期:2008-09-01 00:00:00

  • Fibroblast growth factor complexed to the cytotoxin saporin is growth inhibitory but not cytotoxic for embryonal carcinoma cells.

    abstract::Fibroblast growth factors (FGFs) have been implicated in a number of proliferative lesions, including malignant tumor growth and vascularization. As a result, cytotoxic agents that target cell surface FGF receptors are currently under investigation. Previous reports have shown that conjugation of basic FGF with the ri...


    pub_type: 杂志文章


    authors: Miller K,Wilder PJ,Rizzino A

    更新日期:1993-01-01 00:00:00

  • Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

    abstract::The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...


    pub_type: 杂志文章


    authors: Bayramoglu A,Urhan Kucuk M,Guler HI,Abaci O,Kucukkaya Y,Colak E

    更新日期:2015-01-01 00:00:00

  • The insulin-like growth factor II/mannose-6-phosphate receptor : IGF-II/Man-6-P receptor.

    abstract::Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...


    pub_type: 杂志文章


    authors: Macdonald RG

    更新日期:1989-12-01 00:00:00

  • Impact of chicken-origin cells on adaptation of a low pathogenic influenza virus.

    abstract::Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...


    pub_type: 杂志文章


    authors: Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

    更新日期:2013-05-01 00:00:00

  • Protective effect of bilberry (Vaccinium myrtillus L.) on cisplatin induced ovarian damage in rat.

    abstract::Cisplatin is one of the most effective chemotherapeutic agents but injury may occur at higher doses. The aim of this study was to investigate the effect of bilberry on cisplatin induced toxic effects in rat ovary. Twenty-one female Wistar-Albino rats were utilized to form three groups: In group 1 (control group), each...


    pub_type: 杂志文章


    authors: Pandir D,Kara O,Kara M

    更新日期:2014-08-01 00:00:00

  • The prophylactic use of antibiotics in cell culture.

    abstract::This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...


    pub_type: 杂志文章


    authors: Kuhlmann I

    更新日期:1995-06-01 00:00:00

  • Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes.

    abstract::To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c...


    pub_type: 杂志文章


    authors: Katakura Y,Seto P,Miura T,Ohashi H,Teruya K,Shirahata S

    更新日期:1999-09-01 00:00:00

  • Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances.

    abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...


    pub_type: 杂志文章


    authors: Xiu ZL,Deckwer WD,Zeng AP

    更新日期:1999-05-01 00:00:00

  • Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes.

    abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The ai...


    pub_type: 杂志文章


    authors: Turkez H,Geyikoglu F,Mokhtar YI,Togar B

    更新日期:2012-01-01 00:00:00

  • Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells.

    abstract::The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model system. A variety of monitoring techniques including trypan blue ...


    pub_type: 杂志文章


    authors: Hensler WT,Agathos SN

    更新日期:1994-01-01 00:00:00

  • Identification of the genes specifically expressed in orally tolerized T cells.

    abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...


    pub_type: 杂志文章


    authors: Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

    更新日期:2003-11-01 00:00:00

  • Insights into protective effects of medium additives on animal cells under fluid stresses: the hydrophobic interactions.

    abstract::Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the pr...


    pub_type: 杂志文章


    authors: Wu J

    更新日期:1996-01-01 00:00:00

  • Immunocytochemistry assay in BHK-21 cell line infected with Porcine Sapelovirus.

    abstract::The present study describes an immunocytochemistry (ICC) assay with self-raised hyperimmune sera and a Baby Hamster Kidney-21 (BHK-21) cell line infected with Porcine Sapelovirus (PSV). Sapelovivus/IVRI/SPF-c-6/2015 strain Indian PSV was isolated from the porcine IBRS-2 cell line and investigated for growth on non-por...


    pub_type: 杂志文章


    authors: Kumari S,Singh R,Desingu PA,Ray PK,Taru Sharma G,Saikumar G

    更新日期:2019-06-01 00:00:00

  • New applications of alphavirus-based expression vectors.

    abstract::Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...


    pub_type: 杂志文章


    authors: Boorsma M,Koller D,Renner WA,Bachmann MF

    更新日期:2001-05-01 00:00:00

  • Comparison of specific rates of hybridoma growth and metabolism in batch and continuous cultures.

    abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...


    pub_type: 杂志文章


    authors: Goergen JL,Marc A,Engasser JM

    更新日期:1992-01-01 00:00:00

  • Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method.

    abstract::The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...


    pub_type: 杂志文章


    authors: Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

    更新日期:2015-05-01 00:00:00

  • Genotoxicity of Thermopsis turcica on Allium cepa L. roots revealed by alkaline comet and random amplified polymorphic DNA assays.

    abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...


    pub_type: 杂志文章


    authors: Ciğerci İH,Cenkci S,Kargıoğlu M,Konuk M

    更新日期:2016-08-01 00:00:00

  • Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway.

    abstract::Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...


    pub_type: 杂志文章


    authors: Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

    更新日期:2014-01-01 00:00:00

  • A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

    abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...


    pub_type: 杂志文章


    authors: Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

    更新日期:1998-11-01 00:00:00