Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells.

Abstract:

:Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-rich fractions) were found to enhance the growth and IgM secretion of HB4C5 cells. The promoting activity was found in the commercial LDL. The lipid fraction in YP-2 fraction conjugated with 2-maltosyl-a-cyclodextrin was found to enhance the growth and IgM secretion of HB4C5 cells. Livetin-rich YP-3 and YP-4 fractions had no significant promoting activity. Commercial gamma-livetin and phosphatidyl choline possessed no growth-promoting activity. Phosphatidyl choline enhanced the IgM secretion of HB4C5 cells.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

doi

10.1007/BF00749004

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

149-54

issue

2

eissn

0920-9069

issn

1573-0778

journal_volume

11

pub_type

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