Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells.

abstract：:Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...

journal_title：Cytotechnology

authors： Eugenio M,Campanati L,Müller N,Romão LF,de Souza J,Alves-Leon S,de Souza W,Sant'Anna C

更新日期：2018-12-01 00:00:00

abstract：:This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...

journal_title：Cytotechnology

authors： Ciğerci İH,Cenkci S,Kargıoğlu M,Konuk M

更新日期：2016-08-01 00:00:00

abstract：:Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...

journal_title：Cytotechnology

authors： Marques CL,Rafael MS,Cancela ML,Laizé V

更新日期：2007-09-01 00:00:00

abstract：:Although a variety of assays have been used to examine T cell responses in vitro, standardized ex vivo detection of antigen-specific CD4+ T cells from human circulatory PBMCs remains constrained by low-dimensional characterization outputs and the need for polyclonal, mitogen-induced expansion methods to generate detec...

journal_title：Cytotechnology

authors： Carrio R,Zhang G,Drake DR 3rd,Schanen BC

更新日期：2018-10-01 00:00:00

abstract：:Rats subcutaneously implanted with AH109A hepatoma cells show hyperlipidemia with high concentrations of serum triglyceride and nonesterified fatty acid, suppression of lipoprotein lipase (LPL), and elevation of hormone-sensitive lipase (HSL) activities during the growth of the hepatoma. Supplementation of the diet wi...

journal_title：Cytotechnology

authors： Kawasaki M,Miura Y,Yagasaki K

更新日期：2010-07-01 00:00:00

abstract：:Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...

journal_title：Cytotechnology

authors： Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

更新日期：2018-06-01 00:00:00

abstract：:The advancement of immunocytochemistry (ICC) allows one to observe detailed spatial distribution of cellular antigens, but, with some limitations. Using conventional ICC, it is difficult to distinguish the nuclear localization from cytoplasm, as two large subcellular compartments overlap on the z-axis. In this study, ...

journal_title：Cytotechnology

authors： Moon IS,Lee H,Park SD,Seog DH

更新日期：2010-04-01 00:00:00

abstract：:Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...

journal_title：Cytotechnology

authors： Chen J,Ran Y,Hong C,Chen Z,You Y

更新日期：2010-10-01 00:00:00

abstract：:Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...

journal_title：Cytotechnology

authors： Sununliganon L,Singhatanadgit W

更新日期：2012-01-01 00:00:00

abstract：:Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...

journal_title：Cytotechnology

authors： Ding LH,Iimura E,Saijo K,Hamada H,Ohno T

更新日期：2000-11-01 00:00:00

abstract：:This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...

journal_title：Cytotechnology

authors： Kuhlmann I

更新日期：1995-06-01 00:00:00

abstract：:The growth yields for glucose and glutamine of murine hybridoma cells entrapped in collagen gel particles were examined during the growth phase. The immobilized hybridoma cells were cultivated in a fluidized bed fermenter where the medium was circulating to supply oxygen separately. Procedures to supply an alkaline so...

journal_title：Cytotechnology

authors： Shirai Y,Yamaguchi M,Kobayashi A,Nishi A,Nakamura H,Murakami H

更新日期：1994-01-01 00:00:00

abstract：:The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...

journal_title：Cytotechnology

authors： Theise ND

更新日期：2003-03-01 00:00:00

abstract：:The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...

journal_title：Cytotechnology

authors： Gammell P

更新日期：2007-04-01 00:00:00

abstract：:The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...

journal_title：Cytotechnology

authors： Amadori M,Berneri C

更新日期：1993-01-01 00:00:00

abstract：:Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to th...

journal_title：Cytotechnology

authors： Qi L,Tang Y,He W,Pan H,Jiang W,Wang L,Deng W

更新日期：2017-04-01 00:00:00

abstract：:In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressure by reducing the ...

journal_title：Cytotechnology

authors： Teruya K,Zhang Y,Katakura Y,Shirahata S

更新日期：2002-11-01 00:00:00

abstract：:Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...

journal_title：Cytotechnology

authors： Tao H,Nuo M,Min S

更新日期：2018-02-01 00:00:00

abstract：:We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...

journal_title：Cytotechnology

authors： Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

更新日期：2006-05-01 00:00:00

abstract：:Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...

journal_title：Cytotechnology

authors： Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

更新日期：2003-11-01 00:00:00

abstract：:Achyranthes bidentata (A. bidentata) Blume is a medicinal herb with the property of strengthening bones and muscles and ensuring proper downward flow of blood in terms of the therapeutic theory of traditional medicine. In the present study, the effect of A. bidentata root extract (AE) on osteoblast function was invest...

journal_title：Cytotechnology

authors： Suh KS,Lee YS,Choi EM

更新日期：2014-12-01 00:00:00

abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...

journal_title：Cytotechnology

authors： Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

更新日期：2016-08-01 00:00:00

abstract：:We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...

journal_title：Cytotechnology

authors： Rodas VM,Marques FH,Honda MT,Soares DM,Jorge SA,Antoniazzi MM,Medugno C,Castro ME,Ribeiro BM,Souza ML,Tonso A,Pereira CA

更新日期：2005-06-01 00:00:00

abstract：:The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...

journal_title：Cytotechnology

authors： Nakamura N,Aoki Y,Horiuchi H,Furusawa S,Yamanaka HI,Kitamoto T,Matsuda H

更新日期：2000-03-01 00:00:00

abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...

journal_title：Cytotechnology

authors： Shoyama Y,Tanaka H,Fukuda N

更新日期：1999-09-01 00:00:00

abstract：:The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...

journal_title：Cytotechnology

authors： Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

更新日期：2015-05-01 00:00:00

abstract：:Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...

journal_title：Cytotechnology

authors： Kumalasari ID,Harmayani E,Lestari LA,Raharjo S,Asmara W,Nishi K,Sugahara T

更新日期：2012-03-01 00:00:00

abstract：:The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...

journal_title：Cytotechnology

authors： Uribe E,Venkatesan M,Rose DR,Ewart KV

更新日期：2013-08-01 00:00:00

abstract：:Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...

journal_title：Cytotechnology

authors： Chou CC

更新日期：2014-10-01 00:00:00

abstract：:Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...

journal_title：Cytotechnology

authors： Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

更新日期：2013-05-01 00:00:00