Construction of recombinant monoclonal antibodies from a chicken hybridoma line secreting specific antibody.

abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...

journal_title：Cytotechnology

authors： Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

更新日期：2018-06-01 00:00:00

abstract：:The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...

journal_title：Cytotechnology

authors： Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

更新日期：2003-07-01 00:00:00

abstract：:There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D...

journal_title：Cytotechnology

authors： Szebeni GJ,Tancos Z,Feher LZ,Alfoldi R,Kobolak J,Dinnyes A,Puskas LG

更新日期：2017-04-01 00:00:00

abstract：:Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...

journal_title：Cytotechnology

authors： Chromikova V,Mader A,Steinfellner W,Kunert R

更新日期：2015-03-01 00:00:00

abstract：:Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...

journal_title：Cytotechnology

authors： Koparal AT,Zeytinoglu M

更新日期：2003-11-01 00:00:00

abstract：:Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...

journal_title：Cytotechnology

authors： Weng Z,Jin J,Shao C,Li H

更新日期：2020-04-01 00:00:00

abstract：:A new bioreactor for animal cell cultivation employs two compartments for cells and medium respectively. The two chambers are separated by an ultrafiltration membrane. Cells and solution of collagen or collagen/chitosan mixture were loaded to the cell chamber and were allowed to form gel inside. Contraction of the cel...

journal_title：Cytotechnology

authors： Scholz M,Hu WS

更新日期：1990-09-01 00:00:00

abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...

journal_title：Cytotechnology

authors： Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

更新日期：1991-03-01 00:00:00

abstract：:The development of specific catalytic inhibitors for the serine protease urokinase-type plasminogen activator (uPA) has been hindered due to difficulties in producing sufficient amounts of active recombinant uPA that is catalytically equivalent to native uPA. The purpose of this study was to develop an efficient syste...

journal_title：Cytotechnology

authors： Atkins E,Zamora S,Candia BJ,Baca A,Orlando RA

更新日期：2005-09-01 00:00:00

abstract：:Therapeutic antibody IgG1 has two N-linked oligosaccharide chains bound to the Fc region. The oligosaccharides are of the complex biantennary type, composed of a trimannosyl core structure with the presence or absence of core fucose, bisecting N-acetylglucosamine (GlcNAc), galactose, and terminal sialic acid, which gi...

journal_title：Cytotechnology

authors： Mori K,Iida S,Yamane-Ohnuki N,Kanda Y,Kuni-Kamochi R,Nakano R,Imai-Nishiya H,Okazaki A,Shinkawa T,Natsume A,Niwa R,Shitara K,Satoh M

更新日期：2007-12-01 00:00:00

abstract：:The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...

journal_title：Cytotechnology

authors： Higuchi A,Watanabe T,Noguchi Y,Chang Y,Chen WY,Matsuoka Y

更新日期：2007-07-01 00:00:00

abstract：:The low-metastatic RSV-transformed fibrosarcoma line B77-3T3 and its metastatic variant AA12, selected in vitro, have been analysed by blot and in situ hybridization with v-src and murine c-myc specific probes in order to detect molecular rearrangements underlying the transition from the low-metastatic to the high-met...

journal_title：Cytotechnology

authors： Doneda L,Custode P,Morghen Cde G,Larizza L

更新日期：1987-10-01 00:00:00

abstract：:The UFL-AG-286 cell line, established from embryonic tissue of the lepidopteran insect Anticarsia gemmatalis, has been identified as a good candidate to be used as a cellular substrate in the development of a process for in vitro production of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, a baculovirus w...

journal_title：Cytotechnology

authors： Gioria VV,Jäger V,Claus JD

更新日期：2006-10-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...

journal_title：Cytotechnology

authors： Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

更新日期：2018-12-01 00:00:00

abstract：:Infection of human B cells with Epstein-Barr virus (EBV) induces polyclonal activation in almost all infected cells, but a small proportion of infected cells are transformed to immortalized lymphoblastoid cell lines. Since B cells are activated also by CD40 ligand (CD40L) and Toll-like receptor (TLR) agonists via a si...

journal_title：Cytotechnology

authors： Younesi V,Shirazi FG,Memarian A,Amanzadeh A,Jeddi-Tehrani M,Shokri F

更新日期：2014-01-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to stu...

journal_title：Cytotechnology

authors： Grenade C,Moniotte N,Rompen E,Vanheusden A,Mainjot A,De Pauw-Gillet MC

更新日期：2016-12-01 00:00:00

abstract：:Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...

journal_title：Cytotechnology

authors： Chen J,Ran Y,Hong C,Chen Z,You Y

更新日期：2010-10-01 00:00:00

abstract：:Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...

journal_title：Cytotechnology

authors： Yuan TF

更新日期：2007-06-01 00:00:00

abstract：:The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...

journal_title：Cytotechnology

authors： Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

更新日期：2002-05-01 00:00:00

abstract：:We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...

journal_title：Cytotechnology

authors： Akimaru K,Kuo MT,Furuta K,Suzuki M,Noyori R,Ishikawa T

更新日期：1996-01-01 00:00:00

abstract：:Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...

journal_title：Cytotechnology

authors： Marquis CP,Low KS,Barford JP,Harbour C

更新日期：1989-08-01 00:00:00

abstract：:The growth yields for glucose and glutamine of murine hybridoma cells entrapped in collagen gel particles were examined during the growth phase. The immobilized hybridoma cells were cultivated in a fluidized bed fermenter where the medium was circulating to supply oxygen separately. Procedures to supply an alkaline so...

journal_title：Cytotechnology

authors： Shirai Y,Yamaguchi M,Kobayashi A,Nishi A,Nakamura H,Murakami H

更新日期：1994-01-01 00:00:00

abstract：:Increasing interest has been observed in the use of photobiomodulation (PBM) to enhance the proliferation of stem cells and induce their differentiation. The effects of PBM at two different wavelengths (635 and 809 nm) with three different energy densities (0.5, 1 and 2 J/cm2) on the osteogenic differentiation of adip...

journal_title：Cytotechnology

authors： Bölükbaşı Ateş G,Ak A,Garipcan B,Gülsoy M

更新日期：2020-04-01 00:00:00

abstract：:In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...

journal_title：Cytotechnology

authors： Büntemeyer H,Marzahl R,Lehmann J

更新日期：1994-01-01 00:00:00

abstract：:For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...

journal_title：Cytotechnology

authors： Goergen JL,Marc A,Engasser JM

更新日期：1992-01-01 00:00:00

abstract：:Iodine labeled metaiodobenzylguanidine (MIBG) is a radiopharmaceutical employed for both diagnosis and metabolic radiotherapy of neuroblastoma (NB). Resistance to the radiotherapeutic effects of MIBG is common, due to lack of MIBG accumulation by NB cells. MIBG enters competent cells via the noradrenaline transporter;...

journal_title：Cytotechnology

authors： Montaldo PG,Carbone R,Cornaglia Ferraris P,Ponzoni M

更新日期：1993-01-01 00:00:00

abstract：:This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...

journal_title：Cytotechnology

authors： Xu Y,Zhang J,Ma Y,Han Y,Min J,Liang Y,Zhao D,Qiu J

更新日期：2014-10-01 00:00:00

abstract：:Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...

journal_title：Cytotechnology

authors： Dong C,Shuang F,Weng S,He J

更新日期：2014-12-01 00:00:00

abstract：:Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...

journal_title：Cytotechnology

authors： Cooney CL

更新日期：1995-01-01 00:00:00