Production of immunoglobulin A in different reactor configurations.

Abstract:

:A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bioreactor were found to be glutamine-limited. Ammonia and lactate were produced in significant amounts. IgA productivity was found to be constant and growth associated. Final IgA concentration was similar in both systems. In fed-batch cultures, supplemented with glutamine and glucose, maximum viable cell concentration was increased by 60% and final IgA concentration by 155%. The hollow fiber reactor was able to produce very large amounts of IgA at very high concentrations, similar to the value found in ascites fluid. The productivity ofZac3 is similar to the values reported for IgG-producing cell lines.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Stoll T,Perregaux C,von Stockar U,Marison IW

doi

10.1007/BF00749221

subject

Has Abstract

pub_date

1995-02-01 00:00:00

pages

53-63

issue

1

eissn

0920-9069

issn

1573-0778

journal_volume

17

pub_type

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