Gingival fibroblasts "in vitro" and Down's syndrome.

Abstract:

:Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we examined quantitatively some DS phenotypical aspects to detect possible differences from those of controls. The growth properties of gingival fibroblast cultures from DS patients were more elevated than their ND age-matched controls. There were no differences in plasma membrane polarization and in neutral endopeptidase activity. The succinate-cytochrome C reductase activity decreases in DS fibroblasts compared with ND. Our results outline the difficulties to in using fibroblast cultures as an in vitro system to study premature ageing Down's Syndrome.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Solmi R,Rossetti A,Talassi O,Tomasini GL,Fato R,Estornell E,Lucarini G,Lenaz G,Simonelli L,Brunelli MA

subject

Has Abstract,Author List Incomplete

pub_date

1993-01-01 00:00:00

pages

S59-61

eissn

0920-9069

issn

1573-0778

journal_volume

11 Suppl 1

pub_type

杂志文章
  • The isolation and characterization of putative mesenchymal stem cells from the spiny mouse.

    abstract::The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-012-9443-4

    authors: Dickinson H,Milton P,Jenkin G

    更新日期:2012-10-01 00:00:00

  • Role of a putative tyrosine-O-sulfate receptor in the targeting and/or intracellular transport of tyrosine-sulfated proteins.

    abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1007903315687

    authors: Liu MC,Sakakibara Y,Suiko M

    更新日期:1997-01-01 00:00:00

  • A modification of tooth germ cultivation in vitro and in ovo.

    abstract::Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00135638

    authors: Peterka M,Mandys V,Peterková R

    更新日期:1991-09-01 00:00:00

  • PLCgamma2 Activates CREB-dependent Transcription in PC12 Cells Through Phosphorylation of CREB at Serine 133.

    abstract::The cAMP and Ca(2+) signaling pathways activate the transcription factor CREB through its phosphorylation at Serine 133. Activation of CREB is involved in the regulation of various biological phenomena. To understand further the mechanisms of the regulation of CREB activity in response to activation of the cAMP and Ca...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-005-3763-6

    authors: Iwamoto T,Mamiya N,Masushige S,Kida S

    更新日期:2005-01-01 00:00:00

  • Towards the development of a bioartificial pancreas: immunoisolation and NMR monitoring of mouse insulinomas.

    abstract::A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00762410

    authors: Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

    更新日期:1994-01-01 00:00:00

  • Evaluation of the antiviral effect of new compounds by using cellular cultures.

    abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00736794

    authors: Dianzani F,Antonelli G

    更新日期:1991-02-01 00:00:00

  • Biological response of endothelial cells and its modulation by cytokines: prospects for therapy and bioprocesses.

    abstract::Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1007928203997

    authors: Marique T,Blankaert D,Hendrick V,Raschella A,Declerck B,Alloin C,Teixera-Guerra I,Sandron D,Cherlet M,Parent D,Kirkpatrick C,Van Vooren JP,Wérenne J

    更新日期:1997-11-01 00:00:00

  • Image analysis techniques. The problem of the quantitative evaluation of thechromatin ultrastructure.

    abstract::The application of image analysis methods to conventional thin sections for electron microscopy to analyze the chromatin arrangement are quite limited. We developed a method which utilizes freeze-fractured samples; the results indicate that the method is suitable for identifying the changes in the chromatin arrangemen...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00736824

    authors: Maraldi NM,Marinelli F,Squarzoni S,Santi S,Barbieri M

    更新日期:1991-02-01 00:00:00

  • Effect of oxygen partial pressure on production of animal virus (VSV).

    abstract::The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008060502532

    authors: Lim HS,Chang KH,Kim JH

    更新日期:1999-11-01 00:00:00

  • Optimization of cultivation conditions for monoclonal IgM antibody production by M1A2 hybridoma using artificial neural network.

    abstract::Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00330-5

    authors: Bashokouh F,Abbasiliasi S,Tan JS

    更新日期:2019-07-16 00:00:00

  • Protective effect of bilberry (Vaccinium myrtillus L.) on cisplatin induced ovarian damage in rat.

    abstract::Cisplatin is one of the most effective chemotherapeutic agents but injury may occur at higher doses. The aim of this study was to investigate the effect of bilberry on cisplatin induced toxic effects in rat ovary. Twenty-one female Wistar-Albino rats were utilized to form three groups: In group 1 (control group), each...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9621-z

    authors: Pandir D,Kara O,Kara M

    更新日期:2014-08-01 00:00:00

  • A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.

    abstract::We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008021428969

    authors: Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

    更新日期:1998-11-01 00:00:00

  • Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.

    abstract::Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9795-z

    authors: Ravi M,Kaviya SR,Paramesh V

    更新日期:2016-05-01 00:00:00

  • Use of cell cultures, nuclear and radioanalytical techniques for metallotoxicological studies at the JRC-Ispra.

    abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques.Applications concern the setting of uptake-effect relationships a...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00746046

    authors: Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

    更新日期:1993-01-01 00:00:00

  • Comparison of specific rates of hybridoma growth and metabolism in batch and continuous cultures.

    abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00570891

    authors: Goergen JL,Marc A,Engasser JM

    更新日期:1992-01-01 00:00:00

  • Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV).

    abstract::As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-011-9387-0

    authors: Beas-Catena A,Sánchez-Mirón A,García-Camacho F,Molina-Grima E

    更新日期:2011-12-01 00:00:00

  • New applications of alphavirus-based expression vectors.

    abstract::Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1013192017048

    authors: Boorsma M,Koller D,Renner WA,Bachmann MF

    更新日期:2001-05-01 00:00:00

  • The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent.

    abstract::Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-0018-7

    authors: Bektaş İ,Karaman Ş,Dıraz E,Çelik M

    更新日期:2016-12-01 00:00:00

  • Isolation, culture and differentiation of duck (Anas platyrhynchos) preadipocytes.

    abstract::In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9715-2

    authors: Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JW

    更新日期:2015-10-01 00:00:00

  • The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function.

    abstract::We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02521744

    authors: Parenteau NL,Bilbo P,Nolte CJ,Mason VS,Rosenberg M

    更新日期:1992-01-01 00:00:00

  • In-vitro generation of human adipose tissue derived insulin secreting cells: up-regulation of Pax-6, Ipf-1 and Isl-1.

    abstract::We present a study of up-regulation of genes responsible for pancreatic development in glucose-sensitive insulin-secreting mesenchymal stem cells (IS-MSC) generated and differentiated from human adipose tissue (h-AD), with use of our specific differentiation media and without use of any xenogenic material. Anterior wa...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9573-3

    authors: Dave SD,Vanikar AV,Trivedi HL

    更新日期:2014-03-01 00:00:00

  • Alternative methods in toxicology tests: in vitro toxicity.

    abstract::Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:

    authors: Cinelli S,Falezza A,Meli C,Ciliutti P,Vericat JA

    更新日期:1991-01-01 00:00:00

  • Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.

    abstract::Human umbilical cord blood (hUCB) has been the preferred source of stem cells for the treatment of haematological malignancies and genetic disorders. This is primarily due to its non-invasiveness, high accessibility with relative ease of isolation. Still failures do prevail due to its heterogeneity and lesser frequenc...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9663-2

    authors: Indumathi S,Harikrishnan R,Rajkumar JS,Dhanasekaran M

    更新日期:2015-01-01 00:00:00

  • Signs of deferasirox genotoxicity.

    abstract::Iron overload is a major health problem for patients who have to have continuous blood transfusions. It brings some metabolic problems together. Various iron chelating agents are being used for treatment of hemochromatosis which arises from excess iron accumulation. This study was conducted with the aim of determining...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9617-8

    authors: Ila HB,Topaktas M,Arslan M,Büyükleyla M

    更新日期:2014-08-01 00:00:00

  • Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines.

    abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008159912634

    authors: Kelly HT,Hill E,Reid F,Grant MH,Macdonald C

    更新日期:2000-10-01 00:00:00

  • Effect of some constituents of chicken egg yolk lipoprotein on the growth and IgM production of human-human hybridoma cells and other human-derived cells.

    abstract::Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00749004

    authors: Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

    更新日期:1993-01-01 00:00:00

  • Purification and characterization of alpha-D-galactosidase produced by ADG cell line established from abalon digestive gland.

    abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008175226819

    authors: Kusumoto K,Shirahata S,Kamei Y

    更新日期:2000-07-01 00:00:00

  • DESI-MS as a tool for direct lipid analysis in cultured cells.

    abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9734-z

    authors: Bodzon-Kulakowska A,Cichon T,Golec A,Drabik A,Ner J,Suder P

    更新日期:2015-12-01 00:00:00

  • Real architecture For 3D Tissue (RAFT™) culture system improves viability and maintains insulin and glucagon production of mouse pancreatic islet cells.

    abstract::There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-017-0067-6

    authors: Szebeni GJ,Tancos Z,Feher LZ,Alfoldi R,Kobolak J,Dinnyes A,Puskas LG

    更新日期:2017-04-01 00:00:00

  • High level expression of a frog alpha-amidating enzyme, AE-II, in cultured cells and silkworm larvae using a Bombyx mori nuclear polyhedrosis virus expression vector.

    abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02525492

    authors: Kobayashi J,Imanishi S,Inoue H,Ohsuye K,Yamaichi K,Tsuruoka N,Tanaka S

    更新日期:1992-01-01 00:00:00