Erythropoietin processing in erythropoietic system and central nervous system.

abstract：:The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...

journal_title：Cytotechnology

authors： Nakamura N,Aoki Y,Horiuchi H,Furusawa S,Yamanaka HI,Kitamoto T,Matsuda H

更新日期：2000-03-01 00:00:00

abstract：:The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...

journal_title：Cytotechnology

authors： Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

更新日期：2002-05-01 00:00:00

abstract：:Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...

journal_title：Cytotechnology

authors： Yuan TF

更新日期：2007-06-01 00:00:00

abstract：:Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...

journal_title：Cytotechnology

authors： Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

更新日期：2003-11-01 00:00:00

abstract：:Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...

journal_title：Cytotechnology

authors： Damia G,D'Incalci M

更新日期：1998-09-01 00:00:00

abstract：:This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...

journal_title：Cytotechnology

authors： Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

更新日期：2014-08-01 00:00:00

abstract：:A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...

journal_title：Cytotechnology

authors： Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

更新日期：1993-01-01 00:00:00

abstract：:Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...

journal_title：Cytotechnology

authors： Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

更新日期：2008-09-01 00:00:00

abstract：:Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...

journal_title：Cytotechnology

authors： Cooney CL

更新日期：1995-01-01 00:00:00

abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...

journal_title：Cytotechnology

authors： Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

更新日期：2014-01-01 00:00:00

abstract：:Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...

journal_title：Cytotechnology

authors： Eroğlu HE,Aksoy A,Hamzaoğlu E,Budak U,Albayrak S

更新日期：2009-01-01 00:00:00

abstract：:Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...

journal_title：Cytotechnology

authors： Ma Y,Guo Y,Wu S,Lv Z,Zhang Q,Xie X,Ke Y

更新日期：2018-02-01 00:00:00

abstract：:Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...

journal_title：Cytotechnology

authors： Kelly HT,Hill E,Reid F,Grant MH,Macdonald C

更新日期：2000-10-01 00:00:00

abstract：:With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...

journal_title：Cytotechnology

authors： Guan YH,Kemp RB

更新日期：1999-07-01 00:00:00

abstract：:For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...

journal_title：Cytotechnology

authors： Goergen JL,Marc A,Engasser JM

更新日期：1992-01-01 00:00:00

abstract：:More than 35 years ago double Nobel laureate Linus Carl Pauling published a powerful model of the molecular mechanism of general anesthesia, generally referred to as the hydrate-microcrystal (aqueous-phase) theory. This hypothesis, based on the molecular behavior of water molecules, did not receive serious attention d...

journal_title：Cytotechnology

authors： Nakada T

更新日期：2009-04-01 00:00:00

abstract：:Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...

journal_title：Cytotechnology

authors： Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

更新日期：2018-02-01 00:00:00

abstract：:Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...

journal_title：Cytotechnology

authors： Martínez-Vallespín B,Vahjen W,Zentek J

更新日期：2016-10-01 00:00:00

abstract：:In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...

journal_title：Cytotechnology

authors： He N,Dong Z,Tai D,Liang H,Guo X,Cang M,Liu D

更新日期：2018-08-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00

abstract：:This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...

journal_title：Cytotechnology

authors： Wang G,Zhang W,Jacklin C,Freedman D,Eppstein L,Kadouri A

更新日期：1992-01-01 00:00:00

abstract：:Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

journal_title：Cytotechnology

authors： Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

更新日期：2019-07-12 00:00:00

abstract：:We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...

journal_title：Cytotechnology

authors： Thomou H,Sambani C,Kitsiou P,Politis G

更新日期：1988-07-01 00:00:00

abstract：:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...

journal_title：Cytotechnology

authors： Lim HS,Chang KH,Kim JH

更新日期：1999-11-01 00:00:00

abstract：:The degranulation of basophils stimulated by antigen is one of the allergic mechanisms of immediate hypersensitivity reactions. In a previous study, the β-hexosaminidase release of rat basophilic leukemia (RBL-2H3) cells stimulated by IgE cross-linking was specifically inhibited by ethanol extract of olive milled wast...

journal_title：Cytotechnology

authors： Kishikawa A,Amen Y,Shimizu K

更新日期：2017-04-01 00:00:00

abstract：:Over the past decade, global interest in the development of therapeutic monoclonal antibodies (mAbs) has risen rapidly. As therapeutic agents, antibodies have shown marked efficacy in combatting a range of cancers and immune diseases with high target specificity and low toxicity (Carla Lucia et al. in PLoS ONE 6:e2407...

journal_title：Cytotechnology

authors： Weng Z,Jin J,Shao C,Li H

更新日期：2020-04-01 00:00:00

abstract：:Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...

journal_title：Cytotechnology

authors： Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

更新日期：2008-03-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques.Applications concern the setting of uptake-effect relationships a...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:Oxidative stress plays a vital role in the occurrence and development of intestinal injury. Soluble epoxide hydrolase 2 gene (EPHX2) is a class of hydrolytic enzymes. We aim to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells. IEC-6 cells ...

journal_title：Cytotechnology

authors： Li J,Luo J,Zhang Y,Tang C,Wang J,Chen C

更新日期：2020-02-01 00:00:00

abstract：:Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...

journal_title：Cytotechnology

authors： Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

更新日期：1993-01-01 00:00:00