Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines.


:Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primary hepatocytes with SV40 DNA. In order toassess the suitability of immortalised lines as an alternativeto primary cell cultures we have used RNA arbitrarily primedpolymerase chain reaction to compare gene expression inimmortalised rat hepatocyte cell lines with that in primary rathepatocytes. We have found that differences exist in the RNAtranscripts between fresh and immortalised hepatocyteshighlighting RNA arbitrarily primed polymerase chain reaction asa useful screening method for identifying immortalised lineswhich retain the most ;normal' phenotype in relation to theprimary cells from which they originated.






Kelly HT,Hill E,Reid F,Grant MH,Macdonald C




Has Abstract


2000-10-01 00:00:00












  • Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production.

    abstract::A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, an...


    pub_type: 杂志文章


    authors: Qi YM,Greenfield PF,Reid S

    更新日期:1996-06-01 00:00:00

  • Heat induction of reporter gene expression via the gadd153 promoter and its possible application to hyperthermia treatment of cancer.

    abstract::The use of the gadd153promoter to induce expression of a reporter geneunder heat stress conditions was investigated,since the results of previous studies have suggestedthat the gadd153promoter is likely to be activated by the indirecteffects of hyperthermia, that is, by DNA damage thatoccurs when reactive oxygen speci...


    pub_type: 杂志文章


    authors: Bouhon IA,Ito A,Shinkai M,Honda H,Kobayashi T

    更新日期:2000-07-01 00:00:00

  • Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

    abstract::The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...


    pub_type: 杂志文章


    authors: Tanaka H,Fukuda N,Shoyama Y

    更新日期:1999-03-01 00:00:00

  • Are we prepared for animal cell technology in the 21st century?

    abstract::Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...


    pub_type: 杂志文章


    authors: Cooney CL

    更新日期:1995-01-01 00:00:00

  • Cephalic hedgehog expression is regulated directly by Sox17 in endoderm development of Xenopus laevis.

    abstract::In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...


    pub_type: 杂志文章


    authors: Yagi Y,Ito Y,Kuhara S,Tashiro K

    更新日期:2008-06-01 00:00:00

  • Induction of MRP/GS-X pump and cellular resistance to anticancer prostaglandins.

    abstract::We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...


    pub_type: 杂志文章


    authors: Akimaru K,Kuo MT,Furuta K,Suzuki M,Noyori R,Ishikawa T

    更新日期:1996-01-01 00:00:00

  • Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances.

    abstract::Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...


    pub_type: 杂志文章


    authors: Xiu ZL,Deckwer WD,Zeng AP

    更新日期:1999-05-01 00:00:00

  • Hypoxia enhances proliferation and stemness of human adipose-derived mesenchymal stem cells.

    abstract::The aim of the study was to obtain the highest number of multipotent adipose-derived mesenchymal stem cells (ADMSCs) by using culture conditions which favour cell expansion without loss of mesenchymal stem cells (MSC)-like properties. Based on the assumption that stem cells reside in niches characterized by hypoxic co...


    pub_type: 杂志文章


    authors: Fotia C,Massa A,Boriani F,Baldini N,Granchi D

    更新日期:2015-12-01 00:00:00

  • Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: Epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8.

    abstract::We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...


    pub_type: 杂志文章


    authors: Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

    更新日期:2001-07-01 00:00:00

  • High-level expression of a foreign gene by a recombinant baculovirus with an expanded host range.

    abstract::The usefulness of host range expanded viruses as an expressionvector system was investigated by following the expression ofthe E. coli lacZ gene. The host range expanded recombinantviruses were obtained from Sf-21 or BmN-4 cells coinfected withAutographa californica and Bombyx mori nuclearpolyhedrosis viruses. Among t...


    pub_type: 杂志文章


    authors: Kim HS,Woo SD,Kim WJ,Choi JY,Kang SK

    更新日期:2000-02-01 00:00:00

  • The influence of drinking-water pollution with heavy metal on the expression of IL-4 and IFN-γ in mice by real-time polymerase chain reaction.

    abstract::In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...


    pub_type: 杂志文章


    authors: Radbin R,Vahedi F,Chamani J

    更新日期:2014-10-01 00:00:00

  • Distinct volume distribution of viable and non-viable hybridoma cells: A flow cytometric study.

    abstract::Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...


    pub_type: 杂志文章


    authors: Sen S,Srienc F,Hu WS

    更新日期:1989-05-01 00:00:00

  • The prophylactic use of antibiotics in cell culture.

    abstract::This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...


    pub_type: 杂志文章


    authors: Kuhlmann I

    更新日期:1995-06-01 00:00:00

  • Expression of recombinant Atlantic salmon serum C-type lectin in Drosophila melanogaster Schneider 2 cells.

    abstract::The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...


    pub_type: 杂志文章


    authors: Uribe E,Venkatesan M,Rose DR,Ewart KV

    更新日期:2013-08-01 00:00:00

  • A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein-protein interactions mediated by G protein-coupled receptors.

    abstract::G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay ...


    pub_type: 杂志文章


    authors: Uno N,Fujimoto T,Komoto S,Kurosawa G,Sawa M,Suzuki T,Kazuki Y,Oshimura M

    更新日期:2018-12-01 00:00:00

  • Regulation of hTERT transcription: a target of cellular and viral mechanisms for immortalization and carcinogenesis.

    abstract::A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...


    pub_type: 杂志文章


    authors: Horikawa I,Michishita E,Barrett JC

    更新日期:2004-06-01 00:00:00

  • Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.

    abstract::In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...


    pub_type: 杂志文章


    authors: Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

    更新日期:1989-02-01 00:00:00

  • The effect of glucose and glutamine on the intracellular nucleotide pool and oxygen uptake rate of a murine hybridoma.

    abstract::The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...


    pub_type: 杂志文章


    authors: Barnabé N,Butler M

    更新日期:2000-10-01 00:00:00

  • Image analysis techniques. The problem of the quantitative evaluation of thechromatin ultrastructure.

    abstract::The application of image analysis methods to conventional thin sections for electron microscopy to analyze the chromatin arrangement are quite limited. We developed a method which utilizes freeze-fractured samples; the results indicate that the method is suitable for identifying the changes in the chromatin arrangemen...


    pub_type: 杂志文章


    authors: Maraldi NM,Marinelli F,Squarzoni S,Santi S,Barbieri M

    更新日期:1991-02-01 00:00:00

  • Highly osteogenic PDL stem cell clones specifically express elevated levels of ICAM1, ITGB1 and TERT.

    abstract::Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...


    pub_type: 杂志文章


    authors: Sununliganon L,Singhatanadgit W

    更新日期:2012-01-01 00:00:00

  • Engineering challenges in high density cell culture systems.

    abstract::High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...


    pub_type: 杂志文章


    authors: Ozturk SS

    更新日期:1996-01-01 00:00:00

  • Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes.

    abstract::Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...


    pub_type: 杂志文章


    authors: Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

    更新日期:2018-06-01 00:00:00

  • Influence of aeration-homogenization system in stirred tank bioreactors, dissolved oxygen concentration and pH control mode on BHK-21 cell growth and metabolism.

    abstract::This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...


    pub_type: 杂志文章


    authors: Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

    更新日期:2014-08-01 00:00:00

  • Anin vitro model for the biological evaluation of dental materials using human periodontal ligament cells.

    abstract::Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...


    pub_type: 杂志文章


    authors: Adams AM,Soames JV,Searle RF

    更新日期:1988-02-01 00:00:00

  • Effect of purine supplementation on the growth of salmonid cell lines in different mammalian sera.

    abstract::The Chinook salmon embryo cell line, CHSE-214, grew well in fetal bovine serum (FBS) but poorly in dialyzed (d) FBS. Purines restored most but not all growth-promoting activity to dFBS, which suggests that purines account for a large portion of the dialyzable fraction's growth-promoting activity. CHSE-214 died in newb...


    pub_type: 杂志文章


    authors: Ganassin RC,Bols NC

    更新日期:1992-01-01 00:00:00

  • Epithelial properties of human intestinal Caco-2 cells cultured in a serum-free medium.

    abstract::Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...


    pub_type: 杂志文章


    authors: Hashimoto K,Shimizu M

    更新日期:1993-01-01 00:00:00

  • Features of free software packages in flow cytometry: a comparison between four non-commercial software sources.

    abstract::Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...


    pub_type: 杂志文章


    authors: Sahraneshin Samani F,Moore JK,Khosravani P,Ebrahimi M

    更新日期:2014-08-01 00:00:00

  • Endogenous synthesis of prostacyclin was positively regulated during the maturation phase of cultured adipocytes.

    abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...


    pub_type: 杂志文章


    authors: Rahman MS,Khan F,Syeda PK,Nishimura K,Jisaka M,Nagaya T,Shono F,Yokota K

    更新日期:2014-08-01 00:00:00

  • Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture.

    abstract::The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...


    pub_type: 杂志文章


    authors: Wikarta JM,Kim SM

    更新日期:2016-08-01 00:00:00

  • Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells.

    abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...


    pub_type: 杂志文章


    authors: Han J,Talorete TP,Yamada P,Isoda H

    更新日期:2009-01-01 00:00:00