Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines.

abstract：:Matrine is a bioactive component of the traditional Chinese medical herb Sophora flavescens that has been used in China to treat various kinds of diseases including virus hepatitis. However, the molecular mechanisms underlying its hepatoprotective effects remains elusive. In the present study, primary human hepatocyte...

journal_title：Cytotechnology

authors： Gong X,Gao Y,Guo G,Vondran FW,Schwartlander R,Efimova E,Pless G,Sauera IM,Neuhaus P

更新日期：2015-03-01 00:00:00

abstract：:NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed ...

journal_title：Cytotechnology

authors： Keen MJ,Steward TW

更新日期：1995-10-01 00:00:00

abstract：:Although a variety of assays have been used to examine T cell responses in vitro, standardized ex vivo detection of antigen-specific CD4+ T cells from human circulatory PBMCs remains constrained by low-dimensional characterization outputs and the need for polyclonal, mitogen-induced expansion methods to generate detec...

journal_title：Cytotechnology

authors： Carrio R,Zhang G,Drake DR 3rd,Schanen BC

更新日期：2018-10-01 00:00:00

abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...

journal_title：Cytotechnology

authors： Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

更新日期：2016-08-01 00:00:00

abstract：:IL-5 plays important roles in eosinophil differentiation, expansion, and recruitment. The regulation of IL-5 seems critical for the treatment of eosinophil-mediated allergic reactions. However, the precise mechanisms for IL-5 regulation remain unknown. In this study, we investigated how IL-5 production is regulated. T...

journal_title：Cytotechnology

authors： Hashiguchi M,Kobayashi A,Kashiwakura Y,Kojima H,Kanno Y,Kurosu A,Tokudome S,Kobata T

更新日期：2014-05-01 00:00:00

abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...

journal_title：Cytotechnology

authors： Keenan J,Pearson D,Clynes M

更新日期：2006-03-01 00:00:00

abstract：:Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...

journal_title：Cytotechnology

authors： Schrimpf G,Schröder M,Weitnauer E,Friedl P

更新日期：1994-01-01 00:00:00

abstract：:The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...

journal_title：Cytotechnology

authors： Theise ND

更新日期：2003-03-01 00:00:00

abstract：:The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...

journal_title：Cytotechnology

authors： Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

更新日期：2003-07-01 00:00:00

abstract：:This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...

journal_title：Cytotechnology

authors： Lindl T

更新日期：1996-01-01 00:00:00

abstract：:We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...

journal_title：Cytotechnology

authors： Akimaru K,Kuo MT,Furuta K,Suzuki M,Noyori R,Ishikawa T

更新日期：1996-01-01 00:00:00

abstract：:Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...

journal_title：Cytotechnology

authors： Bodzon-Kulakowska A,Cichon T,Golec A,Drabik A,Ner J,Suder P

更新日期：2015-12-01 00:00:00

abstract：:TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...

journal_title：Cytotechnology

authors： Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

更新日期：2009-07-01 00:00:00

abstract：:This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...

journal_title：Cytotechnology

authors： Xu Y,Zhang J,Ma Y,Han Y,Min J,Liang Y,Zhao D,Qiu J

更新日期：2014-10-01 00:00:00

abstract：:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

journal_title：Cytotechnology

authors： Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

更新日期：1998-03-01 00:00:00

abstract：:A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...

journal_title：Cytotechnology

authors： Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

更新日期：1993-01-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) are key players in liver fibrosis and regeneration via collagen degradation and synthesis. These phenomena involve inflammatory cytokines released from non-parenchymal liver cells such as Kupffer cells. Although the effects of individual cytokines on many cell types have been investigated...

journal_title：Cytotechnology

authors： Inoue A,Obayashi K,Sonoda Y,Nakamura A,Ueno T,Kuhara S,Tashiro K

更新日期：2017-06-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...

journal_title：Cytotechnology

authors： Michelson S

更新日期：1993-01-01 00:00:00

abstract：:In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to ree...

journal_title：Cytotechnology

authors： Fernyhough ME,Vierck JL,Hausman GJ,Mir PS,Okine EK,Dodson MV

更新日期：2004-10-01 00:00:00

abstract：:Nanotechnology is increasingly developing area including more than 700 commercial products such as clothing, food preparation, cosmetics, mechanics, electronics and also health industry. People generally contact with nanoparticles by inhaling from air. Thus, it is becoming important issue to understand harmful effects...

journal_title：Cytotechnology

authors： Türkez H,Arslan ME,Sönmez E,Açikyildiz M,Tatar A,Geyikoğlu F

更新日期：2019-02-01 00:00:00

abstract：:To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...

journal_title：Cytotechnology

authors： Zhou Y,Yi Y,Zhang Z,He J,Zhang Y

更新日期：2003-01-01 00:00:00

abstract：:Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...

journal_title：Cytotechnology

authors： Cooney CL

更新日期：1995-01-01 00:00:00

abstract：:Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...

journal_title：Cytotechnology

authors： Ma Y,Guo Y,Wu S,Lv Z,Zhang Q,Xie X,Ke Y

更新日期：2018-02-01 00:00:00

abstract：:Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...

journal_title：Cytotechnology

authors： Heike Y,Tsuruo T

更新日期：1993-01-01 00:00:00

abstract：:Taking into account that oxidative stress is among the factors causing cancer-related death; chemoprevention which consists in using antioxidant substances such as phenolics could prevent cancer formation and progression. In the present study, phenolic contents and antioxidant activities of methanolic extracts from th...

journal_title：Cytotechnology

authors： Boulaaba M,Tsolmon S,Ksouri R,Han J,Kawada K,Smaoui A,Abdelly C,Isoda H

更新日期：2013-12-01 00:00:00

abstract：:This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...

journal_title：Cytotechnology

authors： Wang G,Zhang W,Jacklin C,Freedman D,Eppstein L,Kadouri A

更新日期：1992-01-01 00:00:00

abstract：:The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...

journal_title：Cytotechnology

authors： Nakamura N,Aoki Y,Horiuchi H,Furusawa S,Yamanaka HI,Kitamoto T,Matsuda H

更新日期：2000-03-01 00:00:00

abstract：:Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...

journal_title：Cytotechnology

authors： Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

更新日期：1993-01-01 00:00:00