Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.

abstract：:It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...

journal_title：Cytotechnology

authors： Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

更新日期：2008-07-01 00:00:00

abstract：:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...

journal_title：Cytotechnology

authors： Nielsen LK,Smyth GK,Greenfield PF

更新日期：1992-01-01 00:00:00

abstract：:The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...

journal_title：Cytotechnology

authors： Bayramoglu A,Urhan Kucuk M,Guler HI,Abaci O,Kucukkaya Y,Colak E

更新日期：2015-01-01 00:00:00

abstract：:Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...

journal_title：Cytotechnology

authors： Azimi M,Niimi T,Yoshida N,Kitagawa Y

更新日期：1999-09-01 00:00:00

abstract：:The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...

journal_title：Cytotechnology

authors： Pichon D,Cudennec B,Huchette S,Djediat C,Renault T,Paillard C,Auzoux-Bordenave S

更新日期：2013-10-01 00:00:00

abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...

journal_title：Cytotechnology

authors： Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

更新日期：1991-03-01 00:00:00

abstract：:In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...

journal_title：Cytotechnology

authors： Moon IS,Lee H,Walikonis RS

更新日期：2013-03-01 00:00:00

abstract：:MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

journal_title：Cytotechnology

authors： Tan PY,Wen LJ,Li HN,Chai SW

更新日期：2020-10-01 00:00:00

abstract：:Human umbilical cord-derived mesenchymal stem cells (hUMSCs) hold strong self-renewal capacity and low immunogenicity, which have attracted attention as potential candidates for bone repair and regeneration. However, insufficient osteogenic differentiation markedly hinders the clinical applications of hUMSCs. In the p...

journal_title：Cytotechnology

authors： Liu J,Wang H,Ren W,Zhou Y,Ye Z,Tan WS

更新日期：2020-10-01 00:00:00

abstract：:Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...

journal_title：Cytotechnology

authors： Damia G,D'Incalci M

更新日期：1998-09-01 00:00:00

abstract：:The growth of rat adrenal nerve cells was remarkably enhanced by supplementing the cultured medium from the human fibroblast cell line, Hs 68. Maximum specific growth rate and length of the neurites were observed as 0.076 (1/hr) and 0.026 mm, respectively in 20% supplement of five day old medium. In adding more than 2...

journal_title：Cytotechnology

authors： Hong JS,Kim DS,Kim SH,Choi DH,Lee JH,Lee HY

更新日期：1998-03-01 00:00:00

abstract：:A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...

journal_title：Cytotechnology

authors： Horikawa I,Michishita E,Barrett JC

更新日期：2004-06-01 00:00:00

abstract：:Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...

journal_title：Cytotechnology

authors： Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

更新日期：2020-06-01 00:00:00

abstract：:A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...

journal_title：Cytotechnology

authors： Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

更新日期：1994-01-01 00:00:00

abstract：:Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...

journal_title：Cytotechnology

authors： Marquis CP,Low KS,Barford JP,Harbour C

更新日期：1989-08-01 00:00:00

abstract：:Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...

journal_title：Cytotechnology

authors： Dong C,Shuang F,Weng S,He J

更新日期：2014-12-01 00:00:00

abstract：:In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...

journal_title：Cytotechnology

authors： Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JW

更新日期：2015-10-01 00:00:00

abstract：:In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...

journal_title：Cytotechnology

authors： Seong JH,Song YS,Joo HW,Park IH,Shen GY,Shin NK,Lee AH,Kwon AM,Lee Y,Kim H,Kim KS

更新日期：2020-10-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...

journal_title：Cytotechnology

authors： Dua R,Ramaswamy S

更新日期：2013-08-01 00:00:00

abstract：:We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...

journal_title：Cytotechnology

authors： Xu Z,Yu L,Lu H,Feng W,Chen L,Zhou J,Yang X,Qi Z

更新日期：2018-12-01 00:00:00

abstract：:The advancement of immunocytochemistry (ICC) allows one to observe detailed spatial distribution of cellular antigens, but, with some limitations. Using conventional ICC, it is difficult to distinguish the nuclear localization from cytoplasm, as two large subcellular compartments overlap on the z-axis. In this study, ...

journal_title：Cytotechnology

authors： Moon IS,Lee H,Park SD,Seog DH

更新日期：2010-04-01 00:00:00

abstract：:Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to th...

journal_title：Cytotechnology

authors： Qi L,Tang Y,He W,Pan H,Jiang W,Wang L,Deng W

更新日期：2017-04-01 00:00:00

abstract：:In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...

journal_title：Cytotechnology

authors： Büntemeyer H,Marzahl R,Lehmann J

更新日期：1994-01-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00

abstract：:Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...

journal_title：Cytotechnology

authors： Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

更新日期：2018-06-01 00:00:00

abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...

journal_title：Cytotechnology

authors： Ribeiro DL,Cilião HL,Specian AFL,Serpeloni JM,De Oliveira MT,Varanda EA,Vilegas W,Saldanha LL,Martínez-López W,Dokkedal AL,Cólus IMS

更新日期：2018-04-01 00:00:00

abstract：:Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...

journal_title：Cytotechnology

authors： Cinelli S,Falezza A,Meli C,Ciliutti P,Vericat JA

更新日期：1991-01-01 00:00:00

abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...

journal_title：Cytotechnology

authors： Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

更新日期：2018-06-01 00:00:00