Promoting effect of rapeseed proteins and peptides on Sf9 insect cell growth.

Abstract:

:The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks associated with the introduction of an ill defined component of bovine origin, additives such as protein hydrolysates from animal sources are still used. An alternative could be the supplementation of culture media with protein hydrolysates derived from plants. In this study, we describe the replacement of lactalbumin hydrolysate with a laboratory produced hydrolysate of rapeseed proteins. Its effect on Sf9 cell growth kinetics, substrate consumption and by-product formation in low-serum or serum-free medium was evaluated. Cells were unable to grow in the presence of a rapeseed protein hydrolysate generated by PTN 3.0 Special((R)) enzyme and containing only 24% of peptides under 1 kDa in size. On the other hand, serum-free medium supplementation with a rapeseed protein hydrolysate obtained with Orientase 90N((R)) enzyme had a strong growth promoting effect, leading to a 60% increase in maximal cell density without affecting cell metabolism. This significant positive effect could be explained by the higher degree of hydrolysis of this digest, with 74% of peptides under 1 kDa in size.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

doi

10.1023/B:CYTO.0000009816.65227.84

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

75-85

issue

2

eissn

0920-9069

issn

1573-0778

journal_volume

42

pub_type

杂志文章
  • Human fetal aorta-derived vascular progenitor cells: identification and potential application in ischemic diseases.

    abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-008-9167-7

    authors: Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

    更新日期:2008-09-01 00:00:00

  • The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent.

    abstract::Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-0018-7

    authors: Bektaş İ,Karaman Ş,Dıraz E,Çelik M

    更新日期:2016-12-01 00:00:00

  • Use of cell cultures, nuclear and radioanalytical techniques for metallotoxicological studies at the JRC-Ispra.

    abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:

    authors: Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

    更新日期:1993-01-01 00:00:00

  • Dental pulp stem cells and osteogenesis: an update.

    abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

    journal_title:Cytotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s10616-018-0225-5

    authors: Mortada I,Mortada R

    更新日期:2018-10-01 00:00:00

  • Uptake and efflux of FL118 and two FL118 derivatives in 3D cell model.

    abstract::Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00322-5

    authors: Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

    更新日期:2019-07-12 00:00:00

  • Features of free software packages in flow cytometry: a comparison between four non-commercial software sources.

    abstract::Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9609-8

    authors: Sahraneshin Samani F,Moore JK,Khosravani P,Ebrahimi M

    更新日期:2014-08-01 00:00:00

  • Cell cycle, cell size and mitochondrial activity of hybridoma cells during batch cultivation.

    abstract::Cell cycle, cell size and rhodamine 123 fluorescence in cell populations of two batch cultures were analysed and quantified with a fluorescence-activated cell sorter (FACS). Two cultures derived from either exponential or stationary phase innocula were investigated in order to demonstrate the dependency of the subsequ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00365929

    authors: al-Rubeai M,Chalder S,Bird R,Emery AN

    更新日期:1991-11-01 00:00:00

  • The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway.

    abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-9995-9

    authors: Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

    更新日期:2016-12-01 00:00:00

  • Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes.

    abstract::Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-018-0213-9

    authors: Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

    更新日期:2018-06-01 00:00:00

  • High level expression of a frog alpha-amidating enzyme, AE-II, in cultured cells and silkworm larvae using a Bombyx mori nuclear polyhedrosis virus expression vector.

    abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02525492

    authors: Kobayashi J,Imanishi S,Inoue H,Ohsuye K,Yamaichi K,Tsuruoka N,Tanaka S

    更新日期:1992-01-01 00:00:00

  • Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line.

    abstract::The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/B:CYTO.0000043413.53044.fa

    authors: Ikonomou L,Bastin G,Schneider YJ,Agathos SN

    更新日期:2004-01-01 00:00:00

  • Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation.

    abstract::Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1016195029359

    authors: Dufresne M,Warocquier-Clérout R

    更新日期:2001-09-01 00:00:00

  • Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.

    abstract::Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9795-z

    authors: Ravi M,Kaviya SR,Paramesh V

    更新日期:2016-05-01 00:00:00

  • Cytogenetic effects of nine Helichrysum taxa in human lymphocytes culture.

    abstract::Helichrysum Mill. (Asteraceae) species have been used in folk medicine for thousands of years in the world. The in vitro cytogenetic effects in human lymphocytes of nine Helichrysum taxa used in Turkey folk medicine were investigated. Blood samples were obtained from healthy donors, non-smoking volunteers, which were ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-009-9193-0

    authors: Eroğlu HE,Aksoy A,Hamzaoğlu E,Budak U,Albayrak S

    更新日期:2009-01-01 00:00:00

  • Optimization of macrophage isolation from the Persian sturgeon and the Caspian kutum fish: a comparative study.

    abstract::The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-018-0256-y

    authors: Rastgar S,Movahedinia A,Salamat N,Salati A,Zabihi E

    更新日期:2018-12-01 00:00:00

  • The transient expression of CHIKV VLP in large stirred tank bioreactors.

    abstract::Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00346-x

    authors: Chen P,Demirji J,Ivleva VB,Horwitz J,Schwartz R,Arnold F

    更新日期:2019-12-01 00:00:00

  • Binucleated HeLa cells are formed by cytokinesis failure in starvation and keep the potential of proliferation.

    abstract::Many cytological studies have reported that the numbers of binucleated cells were elevated in various tumors. However, binucleated cells are observed in not only malignant tumors but also normal tissues. Thus, the clinical significance of binucleated cells is controversial. Here we attempted to elucidate the character...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-015-9869-6

    authors: Nishimura K,Watanabe S,Hayashida R,Sugishima S,Iwasaka T,Kaku T

    更新日期:2016-08-01 00:00:00

  • Anti-proliferative and apoptotic effects of oleuropein and hydroxytyrosol on human breast cancer MCF-7 cells.

    abstract::Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-009-9191-2

    authors: Han J,Talorete TP,Yamada P,Isoda H

    更新日期:2009-01-01 00:00:00

  • Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method.

    abstract::The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9704-5

    authors: Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

    更新日期:2015-05-01 00:00:00

  • Mechanisms of resistance to alkylating agents.

    abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008060720608

    authors: Damia G,D'Incalci M

    更新日期:1998-09-01 00:00:00

  • Highly osteogenic PDL stem cell clones specifically express elevated levels of ICAM1, ITGB1 and TERT.

    abstract::Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-011-9390-5

    authors: Sununliganon L,Singhatanadgit W

    更新日期:2012-01-01 00:00:00

  • Vascular growth factor binding kinetics to the endothelial cell basement membrane, with a kinetics-based correction for substrate binding.

    abstract::Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding ki...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-009-9212-1

    authors: Clyne AM,Edelman ER

    更新日期:2009-07-01 00:00:00

  • Insights into protective effects of medium additives on animal cells under fluid stresses: the hydrophobic interactions.

    abstract::Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the pr...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00353929

    authors: Wu J

    更新日期:1996-01-01 00:00:00

  • A practical approach for intracellular protein delivery.

    abstract::Protein delivery represents a powerful tool for experiments in live cells including studies of protein-protein interactions, protein interference with blocking antibodies, intracellular trafficking and protein or peptide biological functions. Most available reagents dedicated to the protein delivery allow efficient cr...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-007-9102-3

    authors: Weill CO,Biri S,Adib A,Erbacher P

    更新日期:2008-01-01 00:00:00

  • Echinococcus granulosus protoscoleces promotes proliferation and invasion of hepatocellular carcinoma cells.

    abstract::There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00437-0

    authors: Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

    更新日期:2021-02-01 00:00:00

  • Change in growth kinetics of hybridoma cells entrapped in collagen gel affected by alkaline supply.

    abstract::The growth yields for glucose and glutamine of murine hybridoma cells entrapped in collagen gel particles were examined during the growth phase. The immobilized hybridoma cells were cultivated in a fluidized bed fermenter where the medium was circulating to supply oxygen separately. Procedures to supply an alkaline so...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00758178

    authors: Shirai Y,Yamaguchi M,Kobayashi A,Nishi A,Nakamura H,Murakami H

    更新日期:1994-01-01 00:00:00

  • Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: Epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8.

    abstract::We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1014060113186

    authors: Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

    更新日期:2001-07-01 00:00:00

  • Endogenous synthesis of prostacyclin was positively regulated during the maturation phase of cultured adipocytes.

    abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9616-9

    authors: Rahman MS,Khan F,Syeda PK,Nishimura K,Jisaka M,Nagaya T,Shono F,Yokota K

    更新日期:2014-08-01 00:00:00

  • Can macroscopic identification of large ovarian follicles be useful in eliminating follicles with impaired steroid function?

    abstract::"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00758179

    authors: Dudek D

    更新日期:1994-01-01 00:00:00

  • Molecular mechanisms of Bombyx batryticatus ethanol extract inducing gastric cancer SGC-7901 cells apoptosis.

    abstract::Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-017-0102-7

    authors: Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

    更新日期:2017-12-01 00:00:00