Abstract:
:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent studies focused on the infection, virus production and harvest from suspension culture bioreactors. Future studies are planned to address the kinetics of adenovirus production in HEK 293 as well as in other cell lines.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJdoi
10.1023/A:1008021428969subject
Has Abstractpub_date
1998-11-01 00:00:00pages
81-9issue
1-3eissn
0920-9069issn
1573-0778journal_volume
28pub_type
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