Abstract:
:High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The design of the cell retention is the key to performance of high density cell culture systems. Oxygenation and media design are also important for maximizing the cell number. In high density perfusion reactors, variable cell density, and hence the metabolic demand, require constant adjustment of perfusion rates. The use of cell specific perfusion rate (CSPR) control provides a constant environment to the cells resulting in consistent production. On-line measurement of cell density and metabolic activities can be used for the estimation of cell densities and the control of CSPR. Issues related to mass transfer and mixing become more important at high cell densities. Due to the difference in mass transfer coefficients for oxygen and CO(2), a significant accumulation of dissolved CO(2) is experienced with silicone tubing aeration. Also, mixing is observed to decrease at high densities. Base addition, if not properly done, could result in localized cell lysis and poor culture performance. Non-uniform mixing in reactors promotes the heterogeneity of the culture. Cell aggregation results in segregation of the cells within different mixing zones. This paper discusses these issues and makes recommendations for further development of high density cell culture bioreactors.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Ozturk SSdoi
10.1007/BF00353919subject
Has Abstractpub_date
1996-01-01 00:00:00pages
3-16issue
1-3eissn
0920-9069issn
1573-0778journal_volume
22pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00145027
更新日期:1988-07-01 00:00:00
abstract::Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749813
更新日期:1993-01-01 00:00:00
abstract:CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00350396
更新日期:1996-01-01 00:00:00
abstract::In this article, we describe a new method that facilitates to isolate mammalian cells inducible hyper-producing heterologous proteins. This method uses the tetracycline-inducible system to express both the selection marker and the heterologous gene, therefore, allows to increase the selection pressure by reducing the ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945517446
更新日期:2002-11-01 00:00:00
abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9616-9
更新日期:2014-08-01 00:00:00
abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008060720608
更新日期:1998-09-01 00:00:00
abstract::We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00563783
更新日期:1990-11-01 00:00:00
abstract::Previously, it has been shown that the binding of epidermal growth factor (EGF) by a wide range of cells decreases as cell density increases. In this report, we demonstrate that KB cells treated chronically with phorbol esters continue to exhibit decreases in EGF receptor binding as cell density increases. This findin...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00350914
更新日期:1991-10-01 00:00:00
abstract::Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to th...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0056-1
更新日期:2017-04-01 00:00:00
abstract::Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00346-x
更新日期:2019-12-01 00:00:00
abstract::Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9795-z
更新日期:2016-05-01 00:00:00
abstract::Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9151-2
更新日期:2008-03-01 00:00:00
abstract::Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0127-y
更新日期:2018-02-01 00:00:00
abstract::In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9477-7
更新日期:2013-03-01 00:00:00
abstract::The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1022435829894
更新日期:2002-05-01 00:00:00
abstract::With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measu...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008038515285
更新日期:1999-07-01 00:00:00
abstract::The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9173-9
更新日期:2008-09-01 00:00:00
abstract::Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00364994
更新日期:1989-12-01 00:00:00
abstract::Recent studies have indicated that Di-(2-ethylhexyl) phthalate (DEHP), the most commonly used plasticizer in daily-life products, could be dispersed in indoor air and induce human exposure via inhalation. DEHP has been reported to have effects on the respiratory system in both animal and human researches. The toxicity...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0111-6
更新日期:2018-02-01 00:00:00
abstract::A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00749221
更新日期:1995-02-01 00:00:00
abstract::In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9952-7
更新日期:2016-10-01 00:00:00
abstract::The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736814
更新日期:1991-02-01 00:00:00
abstract::Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9637-4
更新日期:2014-10-01 00:00:00
abstract::The neurite outgrowth of PC12 cells on collagen-coated glass plates under light emitting diode (LED) irradiation at several wavelengths (i.e., 455, 470, 525, 600, 630, 880 and 945 nm) was investigated. No neurite outgrowth was observed during cultivation under irradiation from the lamp of an inverted light microscope ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9087-y
更新日期:2007-07-01 00:00:00
abstract::Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1020580818979
更新日期:2001-11-01 00:00:00
abstract::The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02522040
更新日期:1992-01-01 00:00:00
abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00556297
更新日期:1991-03-01 00:00:00
abstract::The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00148808
更新日期:1990-07-01 00:00:00
abstract::Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated dise...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-0047-2
更新日期:2017-02-01 00:00:00
abstract::In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-020-00419-2
更新日期:2020-10-01 00:00:00