Abstract:
:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a container, and storage at -80 degrees C. After thawing and changing the freezing medium to regular growth medium, cell growth was nearly identical to that of cells freshly seeded into a new dish.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura Sdoi
10.1007/BF00556297subject
Has Abstractpub_date
1991-03-01 00:00:00pages
273-7issue
3eissn
0920-9069issn
1573-0778journal_volume
5pub_type
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