Abstract:
:In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading the extracellular vesicles efficiently with the drug of choice. One of the proposed solutions to this problem has been drug loading by electroporation, where small pores are created in the membrane of the extracellular vesicles, hereby allowing for free diffusion of the drug compound into the interior of the vesicle. We investigated the utility of adipose-derived stem cells (ASCs) as an efficient exosome donor cell type with a particular focus on the treatment of glioblastoma multiforme (GBM). In addition, we evaluated electroporation-induced effects on the ASC exosomes with respect to their endogenous potential of stimulating GBM proliferation, and morphological changes to single and multiple ASC exosomes. We found that electroporation does not change the endogenous stimulatory capacity of ASC exosomes on GBM cell proliferation, but mediates adverse morphological changes including aggregation of the exosomes. In order to address this issue, we have successfully optimized the use of a trehalose-containing buffer system as a way of maintaining the structural integrity of the exosomes.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux Mdoi
10.1007/s10616-016-9952-7subject
Has Abstractpub_date
2016-10-01 00:00:00pages
2125-38issue
5eissn
0920-9069issn
1573-0778pii
10.1007/s10616-016-9952-7journal_volume
68pub_type
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