On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells.


:With the increasingly competitive commercial production of target proteins by hybridoma and genetically engineered cells, there is an urgent requirement for biosensors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measured by calorimetry could act as an index for metabolic flow rate. Recombinant CHO cell suspensions producing interferon-gamma were pumped to an on-line flow calorimeter. The results showed that an early reflection of metabolic change is size-specific heat flux obtained from dividing heat flow rate by the capacitance change of the cell suspension, using the on-line probe of a dielectric spectroscope. Comparison of heat flux with glucose and glutamine fluxes indicated that the former most accurately reflected decreased metabolic activity. Possibly this was due to accumulation of lactate and ammonia resulting from catabolic substrates being used as biosynthetic precursors. Thus, the heat flux probe is an ideal on-line biosensor for fed-batch culture. A stoichiometric growth reaction was formulated and data for material and heat fluxes incorporated into it. This showed that cell demand for glucose and glutamine was in the stoichiometric ratio of approximately 3:1 rather than the approximately 5:1 in the medium. It was demonstrated that the set of stoichiometric coefficients in the reaction were related through the extent of reaction (advancement) to overall metabolic activity (flux). The fact that this approach can be used for medium optimisation is the basis for an amino-acid-enriched medium which improved cell growth while decreasing catabolic fluxes.






Guan YH,Kemp RB




Has Abstract


1999-07-01 00:00:00












  • Doxorubicin and 5-fluorouracil resistant hepatic cancer cells demonstrate stem-like properties.

    abstract::The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...


    pub_type: 杂志文章


    authors: Vu NB,Nguyen TT,Tran LC,Do CD,Nguyen BH,Phan NK,Pham PV

    更新日期:2013-08-01 00:00:00

  • Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions.

    abstract::Lymphoid cells expressing sufficient levels of Bcl-2 or E1B-19K are known to resist to induction of apoptosis in glutamine-free or nutrient-limited batch cultures. However, despite the increased viability and prolonged stationary phase achieved in batch culture, product yields are not necessarily improved. Here we hav...


    pub_type: 杂志文章


    authors: Mercille S,Massie B

    更新日期:1998-11-01 00:00:00

  • Utility of NucleoCounter for the chondrocyte count in the collagenase digest of human native cartilage.

    abstract::In cartilage tissue engineering, viable cell numbers should be correctly counted in the collagenase digest of the biopsied cartilage. However, this is a difficult task due to the presence of matrix debris, cell ghosts and their aggregates. To search for the correct cell counting method in this situation, we evaluated ...


    pub_type: 杂志文章


    authors: Yonenaga K,Nishizawa S,Akizawa M,Asawa Y,Fujihara Y,Takato T,Hoshi K

    更新日期:2010-12-01 00:00:00

  • Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.

    abstract::Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...


    pub_type: 杂志文章


    authors: Ravi M,Kaviya SR,Paramesh V

    更新日期:2016-05-01 00:00:00

  • Study of the cytotoxic activity of Styrax camporum extract and its chemical markers, egonol and homoegonol.

    abstract::The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...


    pub_type: 杂志文章


    authors: de Oliveira PF,Damasceno JL,Bertanha CS,Araújo AR,Pauletti PM,Tavares DC

    更新日期:2016-08-01 00:00:00

  • Aldehyde dehydrogenase activity of Wharton jelly mesenchymal stromal cells: isolation and characterization.

    abstract::Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...


    pub_type: 杂志文章


    authors: Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

    更新日期:2019-02-01 00:00:00

  • Intracellular localisation studies of doxorubicin and Victoria Blue BO in EMT6-S and EMT6-R cells using confocal microscopy.

    abstract::The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...


    pub_type: 杂志文章


    authors: Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

    更新日期:2002-05-01 00:00:00

  • Investigation of relationship between IL-6 gene variants and hypertension in Turkish population.

    abstract::Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a poly...


    pub_type: 杂志文章


    authors: Karaman E,Urhan Kucuk M,Bayramoglu A,Uzun Göçmen S,Ercan S,Guler HI,Kucukkaya Y,Erden S

    更新日期:2015-12-01 00:00:00

  • Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures.

    abstract::The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vit...


    pub_type: 杂志文章


    authors: Pichon D,Cudennec B,Huchette S,Djediat C,Renault T,Paillard C,Auzoux-Bordenave S

    更新日期:2013-10-01 00:00:00

  • Vascular growth factor binding kinetics to the endothelial cell basement membrane, with a kinetics-based correction for substrate binding.

    abstract::Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding ki...


    pub_type: 杂志文章


    authors: Clyne AM,Edelman ER

    更新日期:2009-07-01 00:00:00

  • Expression of anchorin CII mRNA by cultured chondrocytes.

    abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...


    pub_type: 杂志文章


    authors: Castagnola P,Cancedda R

    更新日期:1991-02-01 00:00:00

  • Effect of oxygen partial pressure on production of animal virus (VSV).

    abstract::The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...


    pub_type: 杂志文章


    authors: Lim HS,Chang KH,Kim JH

    更新日期:1999-11-01 00:00:00

  • Effects of Carvacrol on a Human Non-Small Cell Lung Cancer (NSCLC) Cell Line, A549.

    abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...


    pub_type: 杂志文章


    authors: Koparal AT,Zeytinoglu M

    更新日期:2003-11-01 00:00:00

  • Lithium chloride promotes neuronal differentiation of rat neural stem cells and enhances neural regeneration in Parkinson's disease model.

    abstract::Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to th...


    pub_type: 杂志文章


    authors: Qi L,Tang Y,He W,Pan H,Jiang W,Wang L,Deng W

    更新日期:2017-04-01 00:00:00

  • Establishment of primary cell cultures from fish calcified tissues.

    abstract::Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...


    pub_type: 杂志文章


    authors: Marques CL,Rafael MS,Cancela ML,Laizé V

    更新日期:2007-09-01 00:00:00

  • Mathematical models for multidrug resistance and its reversal.

    abstract::Mathematical models describing drug resistance are briefly reviewed. One model which describes the molecular function of the P-glycoprotein pump in multidrug resistant (MDR) cell lines has been developed and is presented in detail. The pump is modeled as an energy dependent facilitated diffusion process. A partial dif...


    pub_type: 杂志文章,评审


    authors: Michelson S

    更新日期:1993-01-01 00:00:00

  • Mechanisms of resistance to alkylating agents.

    abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...


    pub_type: 杂志文章


    authors: Damia G,D'Incalci M

    更新日期:1998-09-01 00:00:00

  • Influence of aeration-homogenization system in stirred tank bioreactors, dissolved oxygen concentration and pH control mode on BHK-21 cell growth and metabolism.

    abstract::This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...


    pub_type: 杂志文章


    authors: Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

    更新日期:2014-08-01 00:00:00

  • Succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophages.

    abstract::A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associ...


    pub_type: 杂志文章


    authors: Isoda H,Shinmoto H,Matsumura M,Nakahara T

    更新日期:1995-01-01 00:00:00

  • A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials.

    abstract::In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to stu...


    pub_type: 杂志文章


    authors: Grenade C,Moniotte N,Rompen E,Vanheusden A,Mainjot A,De Pauw-Gillet MC

    更新日期:2016-12-01 00:00:00

  • Sericin in the isolating solution improves the yield of islets isolated from the pancreas.

    abstract::Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This st...


    pub_type: 杂志文章


    authors: Yokoi S,Murakami M,Morikawa M,Goi T,Yamaguchi A,Terada S

    更新日期:2016-12-01 00:00:00

  • Cell Culture Derived AgMNPV Bioinsecticide: Biological Constraints and Bioprocess Issues.

    abstract::We have studied parameters for optimizing the Spodoptera frugiperda (Sf9) cell culture and viral infection for the production of Anticarsia gemmatalis multiple nucleopolyhedrosis virus (AgMNPV) polyhedra inclusion bodies (PIBs) in shaker-Schott or spinner bottles and bioreactors. We have assayed the k(L)a of the syste...


    pub_type: 杂志文章


    authors: Rodas VM,Marques FH,Honda MT,Soares DM,Jorge SA,Antoniazzi MM,Medugno C,Castro ME,Ribeiro BM,Souza ML,Tonso A,Pereira CA

    更新日期:2005-06-01 00:00:00

  • Culture of major pelvic ganglion neurons from adult rat.

    abstract::Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology an...


    pub_type: 杂志文章


    authors: Cheng S,Yang X,Zhang Y,Xiao C

    更新日期:2013-08-01 00:00:00

  • Production of immunoglobulin A in different reactor configurations.

    abstract::A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...


    pub_type: 杂志文章


    authors: Stoll T,Perregaux C,von Stockar U,Marison IW

    更新日期:1995-02-01 00:00:00

  • Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells.

    abstract::Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...


    pub_type: 杂志文章


    authors: Martínez-Vallespín B,Vahjen W,Zentek J

    更新日期:2016-10-01 00:00:00

  • Mouse single oocyte imaging by MALDI-TOF MS for lipidomics.

    abstract::Reproductive cells are a very special kind of material for the analysis. Depending on the species, their dimensions allow for the application of mass spectrometry imaging-based techniques to receive a reasonable data for interpretation of their condition without any additional sample preparation steps, except for typi...


    pub_type: 杂志文章


    authors: Bodzon-Kulakowska A,Arena R,Mielczarek P,Hartman K,Kozoł P,Gibuła-Tarlowska E,Wrobel TP,Gąsior Ł,Polański Z,Ptak GE,Suder P

    更新日期:2020-06-01 00:00:00

  • Antibodies in the study of multiple drug resistance.

    abstract::Multidrug resistance (MDR) is a major problem in cancer chemotherapy. As P-glycoprotein is the key molecule in MDR, many investigators have constructed anti-P-glycoprotein monoclonal antibodies (MAbs). Those antibodies, including MRK16 and C219, were used for elucidation of the mechanism of MDR and for overcoming of M...


    pub_type: 杂志文章,评审


    authors: Heike Y,Tsuruo T

    更新日期:1993-01-01 00:00:00

  • Is there any genetic predisposition of MMP-9 gene C1562T and MTHFR gene C677T polymorphisms with essential hypertension?

    abstract::The current study was conducted to determine whether there is a relation between hypertension and two different polymorphisms, including C1562T of the Matrix metalloproteinase-9 (MMP-9) gene and C677T of the methylenetetrahydrofolate reductase (MTHFR) gene. Genomic DNA obtained from 224 persons (125 patients with hype...


    pub_type: 杂志文章


    authors: Bayramoglu A,Urhan Kucuk M,Guler HI,Abaci O,Kucukkaya Y,Colak E

    更新日期:2015-01-01 00:00:00

  • Expression of recombinant Atlantic salmon serum C-type lectin in Drosophila melanogaster Schneider 2 cells.

    abstract::The Atlantic salmon (Salmo salar) serum lectin (SSL) is a soluble C-type lectin that binds bacteria, including salmon pathogens. This lectin is a cysteine-rich oligomeric protein. Consequently, a Drosophila melanogaster expression system was evaluated for use in expressing SSL. A cDNA encoding SSL was cloned into a ve...


    pub_type: 杂志文章


    authors: Uribe E,Venkatesan M,Rose DR,Ewart KV

    更新日期:2013-08-01 00:00:00

  • Real-time transfer of lentiviral particles by producer cells using an engineered coculture system.

    abstract::Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and ...


    pub_type: 杂志文章


    authors: Timmins LM,Patel RS,Teryek MS,Parekkadan B

    更新日期:2019-10-01 00:00:00