The effect of glucose and glutamine on the intracellular nucleotide pool and oxygen uptake rate of a murine hybridoma.

abstract：:A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...

journal_title：Cytotechnology

authors： Stoll T,Perregaux C,von Stockar U,Marison IW

更新日期：1995-02-01 00:00:00

abstract：:The benzofuran lignans egonol and homoegonol are found in all species of the genus Styrax. Since natural products are important sources of new anticancer drugs, this study evaluated the cytotoxic activity of a hydroalcoholic extract of the stems of S. camporum (SCHE) and their chemical markers, egonol (EG) and homoego...

journal_title：Cytotechnology

authors： de Oliveira PF,Damasceno JL,Bertanha CS,Araújo AR,Pauletti PM,Tavares DC

更新日期：2016-08-01 00:00:00

abstract：:There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...

journal_title：Cytotechnology

authors： Gueret V,Negrete-Virgen JA,Lyddiatt A,Al-Rubeai M

更新日期：2002-01-01 00:00:00

abstract：:ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...

journal_title：Cytotechnology

authors： Kusumoto K,Shirahata S,Kamei Y

更新日期：2000-07-01 00:00:00

abstract：:Chicken egg yolk lipoprotein (YLP) was partially fractionated into some constituents, and the effect of constituents of YLP were examined on the growth and immunoglobulin (IgM and IgG) secretion of a HB4C5 human-human hybridoma cell line cultured in serum-free medium. Among the fractions, YP-1 and YP-2 fractions (LDL-...

journal_title：Cytotechnology

authors： Shinohara K,Fukushima T,Suzuki M,Tsutsumi M,Kobori M,Kong ZL

更新日期：1993-01-01 00:00:00

abstract：:Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...

journal_title：Cytotechnology

authors： Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

更新日期：2018-10-01 00:00:00

abstract：:The degranulation of basophils stimulated by antigen is one of the allergic mechanisms of immediate hypersensitivity reactions. In a previous study, the β-hexosaminidase release of rat basophilic leukemia (RBL-2H3) cells stimulated by IgE cross-linking was specifically inhibited by ethanol extract of olive milled wast...

journal_title：Cytotechnology

authors： Kishikawa A,Amen Y,Shimizu K

更新日期：2017-04-01 00:00:00

abstract：:Chemical genomics is a new research paradigm with importantapplications in drug discovery. It links genomic targets withsmall-molecule chemistries thereby allowing for efficient targetvalidation and lead compound identification. ACADIA'schemical-genomics platform consists of a large and diverse small-moleculelibrary (...

journal_title：Cytotechnology

authors： Hacksell U,Nash N,Burstein ES,Piu F,Croston G,Brann MR

更新日期：2002-01-01 00:00:00

abstract：:This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...

journal_title：Cytotechnology

authors： Xu Y,Zhang J,Ma Y,Han Y,Min J,Liang Y,Zhao D,Qiu J

更新日期：2014-10-01 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The ai...

journal_title：Cytotechnology

authors： Turkez H,Geyikoglu F,Mokhtar YI,Togar B

更新日期：2012-01-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...

journal_title：Cytotechnology

authors： Keenan J,Pearson D,Clynes M

更新日期：2006-03-01 00:00:00

abstract：:A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...

journal_title：Cytotechnology

authors： Escudero T,Coll MD,Fuster C,Egozcue J

更新日期：2002-09-01 00:00:00

abstract：:Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...

journal_title：Cytotechnology

authors： Chen J,Ran Y,Hong C,Chen Z,You Y

更新日期：2010-10-01 00:00:00

abstract：:In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...

journal_title：Cytotechnology

authors： Ding F,Li QQ,Li L,Gan C,Yuan X,Gou H,He H,Han CC,Wang JW

更新日期：2015-10-01 00:00:00

abstract：:Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...

journal_title：Cytotechnology

authors： Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

更新日期：2013-05-01 00:00:00

abstract：:Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...

journal_title：Cytotechnology

authors： Adams AM,Soames JV,Searle RF

更新日期：1988-02-01 00:00:00

abstract：:The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...

journal_title：Cytotechnology

authors： Gammell P

更新日期：2007-04-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00

abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques.Applications concern the setting of uptake-effect relationships a...

journal_title：Cytotechnology

authors： Sabbioni E,Pozzi G,Fischbach M,Bahbouth E

更新日期：1993-01-01 00:00:00

abstract：:Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...

journal_title：Cytotechnology

authors： Cooney CL

更新日期：1995-01-01 00:00:00

abstract：:Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...

journal_title：Cytotechnology

authors： Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

更新日期：1997-01-01 00:00:00

abstract：:Mesenchymal stromal cells (MSCs) are promising tools in regenerative medicine and targeted therapies. Although different origins have been described, there is still huge need to find a valuable source harboring specific subpopulations of MSCs with precise therapeutic functions. Here, we isolated by fluorescence activa...

journal_title：Cytotechnology

authors： Najar M,Crompot E,van Grunsven LA,Dollé L,Lagneaux L

更新日期：2019-02-01 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:Primary cell cultures obtained from embryos of Mizuchopecten yessoensis (Bivalvia) survived for four months. Although the number of cells progressively decreased during the cultivation, mitotic cells were observed both at the first stages and at the end. A possibility of growing marine invertebrates cells in long term...

journal_title：Cytotechnology

authors： Odintsova NA,Khomenko AV

更新日期：1991-05-01 00:00:00

abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...

journal_title：Cytotechnology

authors： Abd El-Hafeez AA,Fujimura T,Kamei R,Hirakawa N,Baba K,Ono K,Kawamoto S

更新日期：2018-06-01 00:00:00

abstract：:Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...

journal_title：Cytotechnology

authors： Kelly HT,Hill E,Reid F,Grant MH,Macdonald C

更新日期：2000-10-01 00:00:00

abstract：:Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...

journal_title：Cytotechnology

authors： Dua R,Ramaswamy S

更新日期：2013-08-01 00:00:00

abstract：:We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...

journal_title：Cytotechnology

authors： Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

更新日期：1991-03-01 00:00:00