Abstract:
:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs (shRNAs). RPS15A knockdown efficiency was validated by quantitative polymerase chain reaction and western blot analysis. Subsequently, the functional effects of RPS15A on proliferation of breast cancer cells were investigated by MTT, colony formation and flow cytometry assays. Functional analysis indicated that RPS15A knockdown could inhibit cell proliferation, induced cell cycle arrest and apoptosis. Mechanism analysis revealed RPS15A mediated apoptosis via activating of caspase-3 and PARP cleavage, upregulating of Bad and BAX and downregulating of Bcl-2. Our preliminary study highlighted the importance of RPS15A in breast cancer growth. The inhibition of RPS15A may be a promising therapeutic target for breast cancer treatment.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Feng W,Liang C,Wang C,Yu X,Li Q,Yang Hdoi
10.1007/s10616-018-0221-9subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1315-1323issue
5eissn
0920-9069issn
1573-0778pii
10.1007/s10616-018-0221-9journal_volume
70pub_type
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