Differential expression of mRNAs encoding laminin chain variants during in vitro development of mouse blastocysts.

Abstract:

:Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and alpha5 mRNAs were additionally expressed after attachment of blastocysts to culture dishes. alpha2 and beta2 mRNAs started to be expressed at the stage of allantois formation. Among ten laminin chains, only the mRNA encoding components of laminin-5 (alpha3, beta3 and gamma2) was missing in embryos up to the stage of allantois formation.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Azimi M,Niimi T,Yoshida N,Kitagawa Y

doi

10.1023/A:1008084508489

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

185-93

issue

1-2

eissn

0920-9069

issn

1573-0778

journal_volume

31

pub_type

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