Genetic engineering of α2,6-sialyltransferase in recombinant CHO cells and its effects on the sialylation of recombinant interferon-γ.


:The CHO cell line has achieved considerable commercial importance as a vehicle for the production of human therapeutic proteins, but is known to lack a functional copy of the gene coding for α2,6-sialyltransferase (EC The cDNA for rat α2,6-ST was expressed in a recombinant CHO cell line making interferon-γ, using a novel in vitro amplification vector. The enzyme was expressed efficiently, and resulted in up to 60% of the total sialic acids on interferon-γ being linked in the α2,6-conformation. This sialic acid linkage distribution was more akin to that seen in natural human glycoproteins. In the most successful cell clones, expression of α2,6-sialyltransferase improved the overall level of sialylation by up to 56%, and had no adverse effects on cell growth, IFN-γ productivity or other aspects of IFN-γ glycosylation. These experiments demonstrate how the glycosylation machinery of rodent cells can be genetically manipulated to replicate human tissues.






Monaco L,Marc A,Eon-Duval A,Acerbis G,Distefano G,Lamotte D,Engasser JM,Soria M,Jenkins N




Has Abstract


1996-01-01 00:00:00












  • Identification of the genes specifically expressed in orally tolerized T cells.

    abstract::Oral tolerance is the systemic immunological unresponsiveness that occurs after feeding protein antigens. Its physiological role is thought to be the prevention of hypersensitivity to food antigens, and its therapeutic use to treat inflammatory diseases has been suggested. Although it has been shown that CD4(+) T cell...


    pub_type: 杂志文章


    authors: Gotoh T,Ise W,Nonaka A,Hamaguchi S,Hachimura S,Kaminogawa S

    更新日期:2003-11-01 00:00:00

  • Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells.

    abstract::Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...


    pub_type: 杂志文章


    authors: Martínez-Vallespín B,Vahjen W,Zentek J

    更新日期:2016-10-01 00:00:00

  • Purification and characterization of lymphocytic clonal growth factor (LCGF) derived from human-human hybridoma SH-76 cells.

    abstract::Human-human hybridoma SH-76 cells were found to produce a factor that supported the growth of lymphocytic cells at low densities. The factor was purified from serum-free conditioned medium of the hybridoma cells by a successive application of ammonium sulfate precipitation, DEAE-Toyopearl, TSK G3000 SW and DEAE-5PW co...


    pub_type: 杂志文章


    authors: Miyata M,Murakami H,Hashizume S,Yamada K,Omura H

    更新日期:1988-11-01 00:00:00

  • Development of human monoclonal antibodies: A review.

    abstract::This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This ...


    pub_type: 杂志文章


    authors: Lindl T

    更新日期:1996-01-01 00:00:00

  • Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

    abstract::The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...


    pub_type: 杂志文章


    authors: Tanaka H,Fukuda N,Shoyama Y

    更新日期:1999-03-01 00:00:00

  • Isolation, culture and identification of pulmonary arterial smooth muscle cells from rat distal pulmonary arteries.

    abstract::The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...


    pub_type: 杂志文章


    authors: Peng G,Xu J,Liu R,Fu Z,Li S,Hong W,Chen J,Li B,Ran P

    更新日期:2017-10-01 00:00:00

  • A modified preplate technique for efficient isolation and proliferation of mice muscle-derived stem cells.

    abstract::We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...


    pub_type: 杂志文章


    authors: Xu Z,Yu L,Lu H,Feng W,Chen L,Zhou J,Yang X,Qi Z

    更新日期:2018-12-01 00:00:00

  • Chaperone and foldase coexpression in the baculovirus-insect cell expression system.

    abstract:CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...


    pub_type: 杂志文章


    authors: Betenbaugh MJ,Ailor E,Whiteley E,Hinderliter P,Hsu TA

    更新日期:1996-01-01 00:00:00

  • Anti-allergic triterpenes isolated from olive milled waste.

    abstract::The degranulation of basophils stimulated by antigen is one of the allergic mechanisms of immediate hypersensitivity reactions. In a previous study, the β-hexosaminidase release of rat basophilic leukemia (RBL-2H3) cells stimulated by IgE cross-linking was specifically inhibited by ethanol extract of olive milled wast...


    pub_type: 杂志文章


    authors: Kishikawa A,Amen Y,Shimizu K

    更新日期:2017-04-01 00:00:00

  • Modified method for effective primary vascular smooth muscle progenitor cell culture from peripheral blood.

    abstract::In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...


    pub_type: 杂志文章


    authors: Seong JH,Song YS,Joo HW,Park IH,Shen GY,Shin NK,Lee AH,Kwon AM,Lee Y,Kim H,Kim KS

    更新日期:2020-10-01 00:00:00

  • Automated time-lapse microscopy and high-resolution tracking of cell migration.

    abstract::We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...


    pub_type: 杂志文章


    authors: Fotos JS,Patel VP,Karin NJ,Temburni MK,Koh JT,Galileo DS

    更新日期:2006-05-01 00:00:00

  • Real-time transfer of lentiviral particles by producer cells using an engineered coculture system.

    abstract::Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and ...


    pub_type: 杂志文章


    authors: Timmins LM,Patel RS,Teryek MS,Parekkadan B

    更新日期:2019-10-01 00:00:00

  • The role of natural indigo dye in alleviation of genotoxicity of sodium dithionite as a reducing agent.

    abstract::Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite...


    pub_type: 杂志文章


    authors: Bektaş İ,Karaman Ş,Dıraz E,Çelik M

    更新日期:2016-12-01 00:00:00

  • Dental pulp stem cells and osteogenesis: an update.

    abstract::Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...


    pub_type: 杂志文章,评审


    authors: Mortada I,Mortada R

    更新日期:2018-10-01 00:00:00

  • Electroporation: an arsenal of application.

    abstract::Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed...


    pub_type: 杂志文章


    authors: Yuan TF

    更新日期:2007-06-01 00:00:00

  • Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.

    abstract::TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...


    pub_type: 杂志文章


    authors: Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

    更新日期:2009-07-01 00:00:00

  • Expression of anchorin CII mRNA by cultured chondrocytes.

    abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...


    pub_type: 杂志文章


    authors: Castagnola P,Cancedda R

    更新日期:1991-02-01 00:00:00

  • Liver stem cells.

    abstract::The capacity of hepatocytes and cholangiocytes to contribute to their own maintenance has long been recognized. More recently, studies have indicated the presence of both intra-hepatic and extra-hepatic stem/progenitor cell populations. The intraorgan compartment probably derives primarily from the biliary tree, most ...


    pub_type: 杂志文章


    authors: Theise ND

    更新日期:2003-03-01 00:00:00

  • A simple method for in situ freezing of anchorage-dependent cells including rat liver parenchymal cells.

    abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...


    pub_type: 杂志文章


    authors: Ohno T,Saijo-Kurita K,Miyamoto-Eimori N,Kurose T,Aoki Y,Yosimura S

    更新日期:1991-03-01 00:00:00

  • Interferon-γ-induced differentiation of human neuroblastoma cells increases cellular uptake and halflife of metaiodobenzylguanidine.

    abstract::Iodine labeled metaiodobenzylguanidine (MIBG) is a radiopharmaceutical employed for both diagnosis and metabolic radiotherapy of neuroblastoma (NB). Resistance to the radiotherapeutic effects of MIBG is common, due to lack of MIBG accumulation by NB cells. MIBG enters competent cells via the noradrenaline transporter;...


    pub_type: 杂志文章


    authors: Montaldo PG,Carbone R,Cornaglia Ferraris P,Ponzoni M

    更新日期:1993-01-01 00:00:00

  • Relative survivability of human osteoblasts is enhanced by 39 °C and ascorbic acid after exposure to photopolymerization ingredients.

    abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...


    pub_type: 杂志文章


    authors: Dua R,Ramaswamy S

    更新日期:2013-08-01 00:00:00

  • Evaluation of monitoring approaches and effects of culture conditions on recombinant protein production in baculovirus-infected insect cells.

    abstract::The baculovirus infection process of Spodoptera frugiperda (Sf9) insect cells in oxygen-controlled bioreactors in serum-free medium was investigated using a recombinant Autographa californica (AcNPV) virus expressing beta-galactosidase enzyme as a model system. A variety of monitoring techniques including trypan blue ...


    pub_type: 杂志文章


    authors: Hensler WT,Agathos SN

    更新日期:1994-01-01 00:00:00

  • NFκB attenuates IL-5 production and upregulates T-box transcription factors in Th2-like T cells.

    abstract::IL-5 plays important roles in eosinophil differentiation, expansion, and recruitment. The regulation of IL-5 seems critical for the treatment of eosinophil-mediated allergic reactions. However, the precise mechanisms for IL-5 regulation remain unknown. In this study, we investigated how IL-5 production is regulated. T...


    pub_type: 杂志文章


    authors: Hashiguchi M,Kobayashi A,Kashiwakura Y,Kojima H,Kanno Y,Kurosu A,Tokudome S,Kobata T

    更新日期:2014-05-01 00:00:00

  • Impact of chicken-origin cells on adaptation of a low pathogenic influenza virus.

    abstract::Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...


    pub_type: 杂志文章


    authors: Shahsavandi S,Ebrahimi MM,Mohammadi A,Zarrin Lebas N

    更新日期:2013-05-01 00:00:00

  • Engineering challenges in high density cell culture systems.

    abstract::High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...


    pub_type: 杂志文章


    authors: Ozturk SS

    更新日期:1996-01-01 00:00:00

  • Production of high titre disabled infectious single cycle (DISC) HSV from a microcarrier culture.

    abstract::Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...


    pub_type: 杂志文章


    authors: Zecchini TA,Smith RJ

    更新日期:1999-07-01 00:00:00

  • Cytotoxic and genotoxic effects of dioxacarb by human peripheral blood lymphocytes CAs and Allium test.

    abstract::Dioxacarb (Elecron, Famid) is a phenyl methylcarbamate insecticide and in vitro cytotoxic and genotoxic effects of this pesticide on human peripheral blood lymphocytes and Allium root meristematic cells were investigated by chromosomal aberrations (CAs) and Allium test. Human lymphocytes were treated with 62.5, 125, 2...


    pub_type: 杂志文章


    authors: Eren Y,Erdoğmuş SF,Akyıl D,Özkara A,Konuk M,Sağlam E

    更新日期:2015-12-01 00:00:00

  • The human vascular endothelial cell line HUV-EC-C harbors the integrated HHV-6B genome which remains stable in long term culture.

    abstract::Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...


    pub_type: 杂志文章


    authors: Shioda S,Kasai F,Ozawa M,Hirayama N,Satoh M,Kameoka Y,Watanabe K,Shimizu N,Tang H,Mori Y,Kohara A

    更新日期:2018-02-01 00:00:00

  • Human fetal aorta-derived vascular progenitor cells: identification and potential application in ischemic diseases.

    abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...


    pub_type: 杂志文章


    authors: Invernici G,Madeddu P,Emanueli C,Parati EA,Alessandri G

    更新日期:2008-09-01 00:00:00

  • Atorvastatin neutralises the thrombin-induced tissue factor expresion in endothelial cells via geranylgeranyl pyrophosphate.

    abstract::Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...


    pub_type: 杂志文章


    authors: Martínez-Sales V,Vila V,Ferrando M,Reganon E

    更新日期:2011-01-01 00:00:00