Abstract：
:Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated diseases. Discovery of new genes and proteins in the differentiation pathway could help to understand the key specific regulators of the adipogenesis. Cytoglobin (Cygb), identified as a new globin family member protein, is expressed in various tissues. Although its interaction with oxygen and nitric oxide indicates the potential role in antioxidant pathways, the exact role remains unclear. In the current study, expression level of Cygb was determined in proliferating and differentiating 3T3-F442A cells by gene expression and protein expression analysis. Results revealed that Cygb expression up-regulated in differentiated cells in parallel with adipogenic differentiation markers; PPARγ, CEBPα and FABP4 expressions. Besides, Cygb overexpression in preadipocytes contributed to the adipogenic differentiation as verified by detection of higher lipid droplets and increased PPARγ, CEBPα and FABP4 expressions with respect to control cells. These findings will shed light on the unknown roles of Cygb in adipogenesis and obesity.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Doğan A,Demirci S,Kıratlı B,Şahin Fdoi
10.1007/s10616-016-0047-2subject
Has Abstractpub_date
2017-02-01 00:00:00pages
157-165issue
1eissn
0920-9069issn
1573-0778pii
10.1007/s10616-016-0047-2journal_volume
69pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract：:Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00364994
更新日期：1989-12-01 00:00:00
abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008051618059
更新日期：1999-09-01 00:00:00
abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0173-5
更新日期：2018-04-01 00:00:00
abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9839-4
更新日期：2016-08-01 00:00:00
abstract：:Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9734-z
更新日期：2015-12-01 00:00:00
abstract：:The bioavailability and action of teas on the invasion of a rat ascites hepatoma cell line, AH109A, were determined and their modes of action were by co-culturing the cancer cells with a rat mesentery-derived mesothelial-cell (M-cell) monolayer in the presence of sera from rats orally given teas and their component, (...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1014008129116
更新日期：2001-07-01 00:00:00
abstract：:Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9609-8
更新日期：2014-08-01 00:00:00
abstract：:Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00744313
更新日期：1995-01-01 00:00:00
abstract：:The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-007-9049-4
更新日期：2007-04-01 00:00:00
abstract：:Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-019-00343-0
更新日期：2019-10-01 00:00:00
abstract：:The aim of this research was a comparative study on the isolation and culture of head kidney macrophages derived from Acipenser persicous and Rutilus frisii kutum as teleost and chondrostei species of fish. The macrophages were isolated by density gradient sedimentation, followed by adherence to a plastic surface. The...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0256-y
更新日期：2018-12-01 00:00:00
abstract：:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008060502532
更新日期：1999-11-01 00:00:00
abstract：:Infection of human B cells with Epstein-Barr virus (EBV) induces polyclonal activation in almost all infected cells, but a small proportion of infected cells are transformed to immortalized lymphoblastoid cell lines. Since B cells are activated also by CD40 ligand (CD40L) and Toll-like receptor (TLR) agonists via a si...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9542-x
更新日期：2014-01-01 00:00:00
abstract：:The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell conce...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00762402
更新日期：1994-01-01 00:00:00
abstract：CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00350396
更新日期：1996-01-01 00:00:00
abstract：:In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-008-9127-2
更新日期：2008-06-01 00:00:00
abstract：:We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00744216
更新日期：1996-01-01 00:00:00
abstract：:Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9811-3
更新日期：2016-08-01 00:00:00
abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0116-1
更新日期：2018-06-01 00:00:00
abstract：:Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotox...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1020580818979
更新日期：2001-11-01 00:00:00
abstract：:We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0262-0
更新日期：2018-12-01 00:00:00
abstract：:Matrine is a bioactive component of the traditional Chinese medical herb Sophora flavescens that has been used in China to treat various kinds of diseases including virus hepatitis. However, the molecular mechanisms underlying its hepatoprotective effects remains elusive. In the present study, primary human hepatocyte...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9680-1
更新日期：2015-03-01 00:00:00
abstract：:Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-015-9857-x
更新日期：2016-08-01 00:00:00
abstract：:Although a variety of assays have been used to examine T cell responses in vitro, standardized ex vivo detection of antigen-specific CD4+ T cells from human circulatory PBMCs remains constrained by low-dimensional characterization outputs and the need for polyclonal, mitogen-induced expansion methods to generate detec...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-018-0222-8
更新日期：2018-10-01 00:00:00
abstract：:A new compound, rotenoid isoflavone glycoside named, 6'-O-beta-D: -glucopyranosyl-12a-hydroxydalpanol was isolated from the methanolic (MeOH) fruit extract of Amorpha fruticosa LINNE by means of multi-stage column chromatography. Immuno-modulatory activities of this new glycoside were compared with the partitioned fra...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-006-9040-5
更新日期：2006-11-01 00:00:00
abstract：:The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of act...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9814-0
更新日期：2016-08-01 00:00:00
abstract：:Alphaviruses are positive stranded RNA viruses that replicate to extremely high titers. Sindbis and Semliki Forest viral vectors are widely used tools for high-level production of recombinant proteins. Recent studies have broadened their scope to vaccine production, gene therapy, and analysis of cell function. Here we...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1013192017048
更新日期：2001-05-01 00:00:00
abstract：:Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00761778
更新日期：1994-01-01 00:00:00
abstract：:The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1007945826228
更新日期：1997-07-01 00:00:00
