Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.

abstract：:Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...

journal_title：Cytotechnology

authors： Marques CL,Rafael MS,Cancela ML,Laizé V

更新日期：2007-09-01 00:00:00

abstract：:IL-5 plays important roles in eosinophil differentiation, expansion, and recruitment. The regulation of IL-5 seems critical for the treatment of eosinophil-mediated allergic reactions. However, the precise mechanisms for IL-5 regulation remain unknown. In this study, we investigated how IL-5 production is regulated. T...

journal_title：Cytotechnology

authors： Hashiguchi M,Kobayashi A,Kashiwakura Y,Kojima H,Kanno Y,Kurosu A,Tokudome S,Kobata T

更新日期：2014-05-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

journal_title：Cytotechnology

authors： Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

更新日期：2016-12-01 00:00:00

abstract：:Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated dise...

journal_title：Cytotechnology

authors： Doğan A,Demirci S,Kıratlı B,Şahin F

更新日期：2017-02-01 00:00:00

abstract：:This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...

journal_title：Cytotechnology

authors： Ciğerci İH,Cenkci S,Kargıoğlu M,Konuk M

更新日期：2016-08-01 00:00:00

abstract：:Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...

journal_title：Cytotechnology

authors： Ravi M,Kaviya SR,Paramesh V

更新日期：2016-05-01 00:00:00

abstract：:Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...

journal_title：Cytotechnology

authors： Dua R,Ramaswamy S

更新日期：2013-08-01 00:00:00

abstract：:Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...

journal_title：Cytotechnology

authors： Tao H,Nuo M,Min S

更新日期：2018-02-01 00:00:00

abstract：:For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...

journal_title：Cytotechnology

authors： Goergen JL,Marc A,Engasser JM

更新日期：1992-01-01 00:00:00

abstract：:There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...

journal_title：Cytotechnology

authors： Gueret V,Negrete-Virgen JA,Lyddiatt A,Al-Rubeai M

更新日期：2002-01-01 00:00:00

abstract：:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...

journal_title：Cytotechnology

authors： Nielsen LK,Smyth GK,Greenfield PF

更新日期：1992-01-01 00:00:00

abstract：:The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...

journal_title：Cytotechnology

authors： Ikonomou L,Bastin G,Schneider YJ,Agathos SN

更新日期：2004-01-01 00:00:00

abstract：:A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal ant...

journal_title：Cytotechnology

authors： Shinmoto H,Matsuo Y,Naganawa Y,Tomita S,Takano-Ishikawa Y

更新日期：2010-08-01 00:00:00

abstract：:Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...

journal_title：Cytotechnology

authors： Adams AM,Soames JV,Searle RF

更新日期：1988-02-01 00:00:00

abstract：:Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...

journal_title：Cytotechnology

authors： Macdonald RG

更新日期：1989-12-01 00:00:00

abstract：:Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...

journal_title：Cytotechnology

authors： Bashokouh F,Abbasiliasi S,Tan JS

更新日期：2019-07-16 00:00:00

abstract：:Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...

journal_title：Cytotechnology

authors： Koparal AT,Zeytinoglu M

更新日期：2003-11-01 00:00:00

abstract：:Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...

journal_title：Cytotechnology

authors： Hashimoto K,Shimizu M

更新日期：1993-01-01 00:00:00

abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...

journal_title：Cytotechnology

authors： Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

更新日期：2016-08-01 00:00:00

abstract：:In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...

journal_title：Cytotechnology

authors： Radbin R,Vahedi F,Chamani J

更新日期：2014-10-01 00:00:00

abstract：:We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...

journal_title：Cytotechnology

authors： Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

更新日期：2001-07-01 00:00:00

abstract：:Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...

journal_title：Cytotechnology

authors： Marquis CP,Low KS,Barford JP,Harbour C

更新日期：1989-08-01 00:00:00

abstract：:Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...

journal_title：Cytotechnology

authors： Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

更新日期：2018-10-01 00:00:00

abstract：:Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...

journal_title：Cytotechnology

authors： Chou CC

更新日期：2014-10-01 00:00:00

abstract：:We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...

journal_title：Cytotechnology

authors： Tuvesson O,Uhe C,Rozkov A,Lüllau E

更新日期：2008-02-01 00:00:00

abstract：:Nanotechnology is increasingly developing area including more than 700 commercial products such as clothing, food preparation, cosmetics, mechanics, electronics and also health industry. People generally contact with nanoparticles by inhaling from air. Thus, it is becoming important issue to understand harmful effects...

journal_title：Cytotechnology

authors： Türkez H,Arslan ME,Sönmez E,Açikyildiz M,Tatar A,Geyikoğlu F

更新日期：2019-02-01 00:00:00

abstract：:ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...

journal_title：Cytotechnology

authors： Kusumoto K,Shirahata S,Kamei Y

更新日期：2000-07-01 00:00:00

abstract：:TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...

journal_title：Cytotechnology

authors： Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

更新日期：2009-07-01 00:00:00

abstract：:The Pseudopimelodidae family comprises 35 species however, cytogenetic studies have been performed in only six species. This study uncovered karyotypic data on Pseudopimelodus pulcher and Microglanis cottoides. Both species possessed 2n = 54, with 20m + 16sm + 10st + 8a and FN = 100 for P. pulcher and 30m + 14sm + 6st...

journal_title：Cytotechnology

authors： Gouveia JG,de Moraes VP,Pires LB,da Rosa R,Dias AL

更新日期：2015-03-01 00:00:00

abstract：:The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...

journal_title：Cytotechnology

authors： Wikarta JM,Kim SM

更新日期：2016-08-01 00:00:00