Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.


:In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture conditions. By using this new apparatus with continuous regulation of infused interleukin 2, nutritional medium, and pO(2) and pCO(2), yields of 2×10(7) cells/ml were achieved and maintained for more than 21 days. These cells also showed a significant increase of LAK activity on a per cell basis.






Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y




Has Abstract


1989-02-01 00:00:00












  • Establishment of primary cell cultures from fish calcified tissues.

    abstract::Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...


    pub_type: 杂志文章


    authors: Marques CL,Rafael MS,Cancela ML,Laizé V

    更新日期:2007-09-01 00:00:00

  • NFκB attenuates IL-5 production and upregulates T-box transcription factors in Th2-like T cells.

    abstract::IL-5 plays important roles in eosinophil differentiation, expansion, and recruitment. The regulation of IL-5 seems critical for the treatment of eosinophil-mediated allergic reactions. However, the precise mechanisms for IL-5 regulation remain unknown. In this study, we investigated how IL-5 production is regulated. T...


    pub_type: 杂志文章


    authors: Hashiguchi M,Kobayashi A,Kashiwakura Y,Kojima H,Kanno Y,Kurosu A,Tokudome S,Kobata T

    更新日期:2014-05-01 00:00:00

  • The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway.

    abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...


    pub_type: 杂志文章


    authors: Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

    更新日期:2016-12-01 00:00:00

  • Cytoglobin: a potential marker for adipogenic differentiation in preadipocytes in vitro.

    abstract::Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated dise...


    pub_type: 杂志文章


    authors: Doğan A,Demirci S,Kıratlı B,Şahin F

    更新日期:2017-02-01 00:00:00

  • Genotoxicity of Thermopsis turcica on Allium cepa L. roots revealed by alkaline comet and random amplified polymorphic DNA assays.

    abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...


    pub_type: 杂志文章


    authors: Ciğerci İH,Cenkci S,Kargıoğlu M,Konuk M

    更新日期:2016-08-01 00:00:00

  • Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures.

    abstract::Advancements in cell cultures are occurring at a rapid pace, an important direction is culturing cells in 3D conditions. We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. The differences in culture phases, susceptibility to cisplatin-in...


    pub_type: 杂志文章


    authors: Ravi M,Kaviya SR,Paramesh V

    更新日期:2016-05-01 00:00:00

  • Relative survivability of human osteoblasts is enhanced by 39 °C and ascorbic acid after exposure to photopolymerization ingredients.

    abstract::Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...


    pub_type: 杂志文章


    authors: Dua R,Ramaswamy S

    更新日期:2013-08-01 00:00:00

  • Sufentanil protects the rat myocardium against ischemia-reperfusion injury via activation of the ERK1/2 pathway.

    abstract::Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...


    pub_type: 杂志文章


    authors: Tao H,Nuo M,Min S

    更新日期:2018-02-01 00:00:00

  • Comparison of specific rates of hybridoma growth and metabolism in batch and continuous cultures.

    abstract::For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...


    pub_type: 杂志文章


    authors: Goergen JL,Marc A,Engasser JM

    更新日期:1992-01-01 00:00:00

  • Rapid titration of adenoviral infectivity by flow cytometry in batch culture of infected HEK293 cells.

    abstract::There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...


    pub_type: 杂志文章


    authors: Gueret V,Negrete-Virgen JA,Lyddiatt A,Al-Rubeai M

    更新日期:2002-01-01 00:00:00

  • Accuracy of the endpoint assay for virus titration.

    abstract::The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...


    pub_type: 杂志文章


    authors: Nielsen LK,Smyth GK,Greenfield PF

    更新日期:1992-01-01 00:00:00

  • Effect of Partial Medium Replacement on Cell Growth and Protein Production for the High-Fivetrade mark insect cell line.

    abstract::The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...


    pub_type: 杂志文章


    authors: Ikonomou L,Bastin G,Schneider YJ,Agathos SN

    更新日期:2004-01-01 00:00:00

  • Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody 92-2.

    abstract::A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal ant...


    pub_type: 杂志文章


    authors: Shinmoto H,Matsuo Y,Naganawa Y,Tomita S,Takano-Ishikawa Y

    更新日期:2010-08-01 00:00:00

  • Anin vitro model for the biological evaluation of dental materials using human periodontal ligament cells.

    abstract::Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...


    pub_type: 杂志文章


    authors: Adams AM,Soames JV,Searle RF

    更新日期:1988-02-01 00:00:00

  • The insulin-like growth factor II/mannose-6-phosphate receptor : IGF-II/Man-6-P receptor.

    abstract::Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...


    pub_type: 杂志文章


    authors: Macdonald RG

    更新日期:1989-12-01 00:00:00

  • Optimization of cultivation conditions for monoclonal IgM antibody production by M1A2 hybridoma using artificial neural network.

    abstract::Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...


    pub_type: 杂志文章


    authors: Bashokouh F,Abbasiliasi S,Tan JS

    更新日期:2019-07-16 00:00:00

  • Effects of Carvacrol on a Human Non-Small Cell Lung Cancer (NSCLC) Cell Line, A549.

    abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...


    pub_type: 杂志文章


    authors: Koparal AT,Zeytinoglu M

    更新日期:2003-11-01 00:00:00

  • Epithelial properties of human intestinal Caco-2 cells cultured in a serum-free medium.

    abstract::Human intestinal Caco-2 cells were cultured under serum-free conditions on an insoluble collagen and FCS matrix (Caco-2-SF), and a comparison was made between several characteristics of Caco-2 and Caco-2-SF cells. Their morphological appearance was identical. Slight differences were found in cell growth and expression...


    pub_type: 杂志文章


    authors: Hashimoto K,Shimizu M

    更新日期:1993-01-01 00:00:00

  • Assessment of the in vitro cytotoxicity and in vivo anti-tumor activity of the alcoholic stem bark extract/fractions of Mimusops elengi Linn.

    abstract::Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...


    pub_type: 杂志文章


    authors: Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

    更新日期:2016-08-01 00:00:00

  • The influence of drinking-water pollution with heavy metal on the expression of IL-4 and IFN-γ in mice by real-time polymerase chain reaction.

    abstract::In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...


    pub_type: 杂志文章


    authors: Radbin R,Vahedi F,Chamani J

    更新日期:2014-10-01 00:00:00

  • Molecular analysis of cross-reactive human monoclonal antibody AE6F4 generated by in vitro immunization: Epitope mapping of AE6F4 antibody on 14-3-3 family proteins and cytokeratin 8.

    abstract::We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...


    pub_type: 杂志文章


    authors: Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata S

    更新日期:2001-07-01 00:00:00

  • Agitation and aeration effects in suspension mammalian cell cultures.

    abstract::Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...


    pub_type: 杂志文章


    authors: Marquis CP,Low KS,Barford JP,Harbour C

    更新日期:1989-08-01 00:00:00

  • Fibroblast growth factor 21 induces lipolysis more efficiently than it suppresses lipogenesis in goat adipocytes.

    abstract::Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...


    pub_type: 杂志文章


    authors: Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

    更新日期:2018-10-01 00:00:00

  • Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis.

    abstract::Although the insect cell/baculovirus system is an important expression platform for recombinant protein production, our understanding of insect cell metabolism with respect to enhancing cell growth capability and productivity is still limited. Moreover, different host insect cell lines may have different growth charac...


    pub_type: 杂志文章


    authors: Chou CC

    更新日期:2014-10-01 00:00:00

  • Development of a generic transient transfection process at 100 L scale.

    abstract::We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...


    pub_type: 杂志文章


    authors: Tuvesson O,Uhe C,Rozkov A,Lüllau E

    更新日期:2008-02-01 00:00:00

  • Synthesis, characterization and cytotoxicity of boron nitride nanoparticles: emphasis on toxicogenomics.

    abstract::Nanotechnology is increasingly developing area including more than 700 commercial products such as clothing, food preparation, cosmetics, mechanics, electronics and also health industry. People generally contact with nanoparticles by inhaling from air. Thus, it is becoming important issue to understand harmful effects...


    pub_type: 杂志文章


    authors: Türkez H,Arslan ME,Sönmez E,Açikyildiz M,Tatar A,Geyikoğlu F

    更新日期:2019-02-01 00:00:00

  • Purification and characterization of alpha-D-galactosidase produced by ADG cell line established from abalon digestive gland.

    abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...


    pub_type: 杂志文章


    authors: Kusumoto K,Shirahata S,Kamei Y

    更新日期:2000-07-01 00:00:00

  • Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis.

    abstract::TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...


    pub_type: 杂志文章


    authors: Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

    更新日期:2009-07-01 00:00:00

  • Comparative cytogenetics between two species of the family Pseudopimelodidae (Siluriformes): occurrence of natural triploidy and supernumerary chromosomes.

    abstract::The Pseudopimelodidae family comprises 35 species however, cytogenetic studies have been performed in only six species. This study uncovered karyotypic data on Pseudopimelodus pulcher and Microglanis cottoides. Both species possessed 2n = 54, with 20m + 16sm + 10st + 8a and FN = 100 for P. pulcher and 30m + 14sm + 6st...


    pub_type: 杂志文章


    authors: Gouveia JG,de Moraes VP,Pires LB,da Rosa R,Dias AL

    更新日期:2015-03-01 00:00:00

  • Nitric oxide synthesis inhibition and cytotoxicity of Korean horse mussel Modiolus modiolus extracts on cancer cells in culture.

    abstract::The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...


    pub_type: 杂志文章


    authors: Wikarta JM,Kim SM

    更新日期:2016-08-01 00:00:00