Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus.

Abstract:

:In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture conditions. By using this new apparatus with continuous regulation of infused interleukin 2, nutritional medium, and pO(2) and pCO(2), yields of 2×10(7) cells/ml were achieved and maintained for more than 21 days. These cells also showed a significant increase of LAK activity on a per cell basis.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Kohgo Y,Sakamaki S,Kanisawa Y,Nojiri S,Ueno Y,Itoh Y,Takahashi M,Sasagawa Y,Hosoi S,Sato S,Niitsu Y

doi

10.1007/BF00365414

subject

Has Abstract

pub_date

1989-02-01 00:00:00

pages

49-57

issue

1

eissn

0920-9069

issn

1573-0778

journal_volume

2

pub_type

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