Abstract:
:Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. The distribution based on small-angle light scattering is similar to the distribution based on volume as measured by Elzone particle counter. It thus appears that viable cells form the population with a larger mean cell volume. The results also indicate that the volume of viable cells decreases during the cultivation while that of dead cells remains relatively constant.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Sen S,Srienc F,Hu WSdoi
10.1007/BF00386140subject
Has Abstractpub_date
1989-05-01 00:00:00pages
85-94issue
2eissn
0920-9069issn
1573-0778journal_volume
2pub_type
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