Effect of oxygen partial pressure on production of animal virus (VSV).

Abstract:

:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies that oxygen partial pressure during viral production has to be low. In low oxygen partial pressure, malondialdehyde concentration was decreased about 5 fold. Thus, low oxygen partial pressure allowed the reduced reactive oxygen species to be evolved, possibly by decreasing the random oxidation of the produced viral protein and membrane from the host cell.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Lim HS,Chang KH,Kim JH

doi

10.1023/A:1008060502532

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

265-70

issue

3

eissn

0920-9069

issn

1573-0778

journal_volume

31

pub_type

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