Abstract:
:Mitochondrial dysfunction has been identified as one of the primary factors contributing to liver diseases. Pathways that control mitochondrial biogenesis are potential therapeutic targets for the amelioration of hepatocyte dysfunction and liver disease. Research on natural pharmacological agents that ameliorate liver diseases has intensified over the last two decades. Cyanidin-3-glucoside (Cy3g), a dietary flavonoid compound extracted from a wide variety of fruits and vegetables, reportedly has several beneficial health effects. In this study, we used an adult human hepatoma cell line (HuH7) to investigate the effects of the Cy3g polyphenolic compound on mitochondrial function and biogenesis in vitro. An increase in intracellular mitochondrial reductase levels was observed after treatment with Cy3g, but cytotoxicity was not induced. In addition, mitochondrial membrane potential and ATP production were increased following Cy3g treatment. Cy3g treatment also resulted in a dose- and time-dependent upregulation of the gene expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), a transcription factor considered a master regulator of mitochondrial biogenesis and metabolism. Additionally, the expression of sirtuin 1 (SIRT1), which plays a key role in deacetylating PGC-1α, was also increased in a dose- and time-dependent manner. Cy3g treatment also increased the expression of downstream PGC-1α genes, nuclear respiratory factor 1 and mitochondrial transcription factor A (TFAM). Our results suggest that Cy3g has potential as a hepatoprotective therapeutic agent that enhances mitochondrial function and biogenesis in hepatocytes.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Mogalli R,Matsukawa T,Shimomura O,Isoda H,Ohkohchi Ndoi
10.1007/s10616-018-0242-4subject
Has Abstractpub_date
2018-12-01 00:00:00pages
1519-1528issue
6eissn
0920-9069issn
1573-0778pii
10.1007/s10616-018-0242-4journal_volume
70pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007903315687
更新日期:1997-01-01 00:00:00
abstract::We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744323
更新日期:1995-01-01 00:00:00
abstract::Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effect...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00133241
更新日期:1989-08-01 00:00:00
abstract::The efficacy of hepatocellular carcinoma (HCC) treatment is very low because of the high percentage of recurrence and resistance to anticancer agents. Hepatic cancer stem cells (HCSCs) are considered the origin of such recurrence and resistance. Our aim was to evaluate the stemness of doxorubicin and 5-fluorouracil re...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9511-9
更新日期:2013-08-01 00:00:00
abstract::In cartilage tissue engineering, viable cell numbers should be correctly counted in the collagenase digest of the biopsied cartilage. However, this is a difficult task due to the presence of matrix debris, cell ghosts and their aggregates. To search for the correct cell counting method in this situation, we evaluated ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9304-y
更新日期:2010-12-01 00:00:00
abstract::A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00365489
更新日期:1990-05-01 00:00:00
abstract::Understanding the growth dynamics of influenza viruses is an essential step in virus replication and cell-adaptation. The aim of this study was to elucidate the growth kinetic of a low pathogenic avian influenza H9N2 subtype in chicken embryo fibroblast (CEF) and chicken tracheal epithelial (CTE) cells during consecut...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9495-5
更新日期:2013-05-01 00:00:00
abstract::To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0221-9
更新日期:2018-10-01 00:00:00
abstract::In the present study, we isolated preadipocytes from the adipose tissue of Peking duck and subsequently cultured them in vitro. Cell counting kit-8 assay was employed to establish the growth curve of duck primary preadipocytes. Meanwhile, after the cells reaching full confluency, they were induced to differentiate int...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9715-2
更新日期:2015-10-01 00:00:00
abstract::Celecoxib is a selective cyclooxygenase-2 (COX-2) inhibitor with antitumor and antiangiogenic activities. To investigate the effects of celecoxib on nasopharyngeal carcinoma (NPC), HNE-1 cells were treated with celecoxib at various concentrations. MTT assay, migration assay and invasion assay were performed to observe...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9296-7
更新日期:2010-10-01 00:00:00
abstract::Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918-940, 2018). Chikungunya Virus-like...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00346-x
更新日期:2019-12-01 00:00:00
abstract::This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb (Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC50 and 2×EC50 values were 8 and 16 mg/ml concentrations...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9835-8
更新日期:2016-08-01 00:00:00
abstract::Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007919921991
更新日期:1997-01-01 00:00:00
abstract::High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00353919
更新日期:1996-01-01 00:00:00
abstract::A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023945218123
更新日期:2002-09-01 00:00:00
abstract::A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9289-6
更新日期:2010-08-01 00:00:00
abstract::Large scale animal cell culture for the production of complex therapeutic proteins has been a major success of the biotechnology industry. Today, approximately half of the $ 5 billion annual turnover of the biotechnology industry is based upon this technology, in many cases with reactors of more than 10 m(3). As we lo...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744313
更新日期:1995-01-01 00:00:00
abstract::Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primar...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008159912634
更新日期:2000-10-01 00:00:00
abstract::We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0262-0
更新日期:2018-12-01 00:00:00
abstract::Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9118-8
更新日期:2008-05-01 00:00:00
abstract::The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02215666
更新日期:1996-01-01 00:00:00
abstract::Lymphoid cells expressing sufficient levels of Bcl-2 or E1B-19K are known to resist to induction of apoptosis in glutamine-free or nutrient-limited batch cultures. However, despite the increased viability and prolonged stationary phase achieved in batch culture, product yields are not necessarily improved. Here we hav...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008054403470
更新日期:1998-11-01 00:00:00
abstract::Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::We developed a simple method for freezing anchorage-dependent cells, including primary cultured rat liver parenchymal cells, without detaching the cells from the culture dish. The method consists of preculture of the cells to confluence, changing the growth medium to a conventional freezing medium, packaging in a cont...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00556297
更新日期:1991-03-01 00:00:00
abstract::The relatively recent discovery of miRNAs has added a completely new dimension to the study of the regulation of gene expression. The mechanism of action of miRNAs, the conservation between diverse species and the fact that each miRNA can regulate a number of targets and phenotypes clearly indicates the importance of ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9049-4
更新日期:2007-04-01 00:00:00
abstract::Rats subcutaneously implanted with AH109A hepatoma cells show hyperlipidemia with high concentrations of serum triglyceride and nonesterified fatty acid, suppression of lipoprotein lipase (LPL), and elevation of hormone-sensitive lipase (HSL) activities during the growth of the hepatoma. Supplementation of the diet wi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-010-9282-0
更新日期:2010-07-01 00:00:00
abstract::The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02522040
更新日期:1992-01-01 00:00:00
abstract::Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00135638
更新日期:1991-09-01 00:00:00
abstract::We present a study of up-regulation of genes responsible for pancreatic development in glucose-sensitive insulin-secreting mesenchymal stem cells (IS-MSC) generated and differentiated from human adipose tissue (h-AD), with use of our specific differentiation media and without use of any xenogenic material. Anterior wa...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9573-3
更新日期:2014-03-01 00:00:00
abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736794
更新日期:1991-02-01 00:00:00