Effect of new rotenoid glycoside from the fruits of Amorpha fruticosa LINNE on the growth of human immune cells.

abstract：:Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...

journal_title：Cytotechnology

authors： Abu-Absi SF,Hansen LK,Hu WS

更新日期：2004-07-01 00:00:00

abstract：:Sufentanil, a lipophilic opioid, is the most frequently used clinical drug for ischemic heart disease. The effects of sufentanil on MAPK signaling in ischemic heart disease were explored. The effects of sufentanil on ischemia-reperfusion (IR)-induced myocardial injury in a rat model were examined. The serum levels of ...

journal_title：Cytotechnology

authors： Tao H,Nuo M,Min S

更新日期：2018-02-01 00:00:00

abstract：:As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...

journal_title：Cytotechnology

authors： Beas-Catena A,Sánchez-Mirón A,García-Camacho F,Molina-Grima E

更新日期：2011-12-01 00:00:00

abstract：:Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...

journal_title：Cytotechnology

authors： Schrimpf G,Schröder M,Weitnauer E,Friedl P

更新日期：1994-01-01 00:00:00

abstract：:Obesity, mainly characterized by the excess fat storage, is a global health problem resulting in serious morbidity and mortality. Identification of molecular mechanisms in adipogenic differentiation pathway might lead to development of new strategies for diagnosis, prevention and therapy of obesity and associated dise...

journal_title：Cytotechnology

authors： Doğan A,Demirci S,Kıratlı B,Şahin F

更新日期：2017-02-01 00:00:00

abstract：:Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...

journal_title：Cytotechnology

authors： Cinelli S,Falezza A,Meli C,Ciliutti P,Vericat JA

更新日期：1991-01-01 00:00:00

abstract：:Iodine labeled metaiodobenzylguanidine (MIBG) is a radiopharmaceutical employed for both diagnosis and metabolic radiotherapy of neuroblastoma (NB). Resistance to the radiotherapeutic effects of MIBG is common, due to lack of MIBG accumulation by NB cells. MIBG enters competent cells via the noradrenaline transporter;...

journal_title：Cytotechnology

authors： Montaldo PG,Carbone R,Cornaglia Ferraris P,Ponzoni M

更新日期：1993-01-01 00:00:00

abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...

journal_title：Cytotechnology

authors： Keenan J,Pearson D,Clynes M

更新日期：2006-03-01 00:00:00

abstract：:Disabled Infectious Single Cycle (DISC) HSV-2 has been cultured in the complimentary cell line CR2 to provide high titre bulk material suitable for the purification of the virus as a live viral vaccine. CR2 cells are cultured on the microcarrier Cytodex-1 at 5 g l-1 in small scale (1 l) and larger scale (15 l) reactor...

journal_title：Cytotechnology

authors： Zecchini TA,Smith RJ

更新日期：1999-07-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...

journal_title：Cytotechnology

authors： Wirth M,Berthold E,Grashoff M,Pfützner H,Schubert U,Hauser H

更新日期：1994-01-01 00:00:00

abstract：:The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented w...

journal_title：Cytotechnology

authors： Sadeesh EM,Selokar NL,Balhara AK,Yadav PS

更新日期：2016-10-01 00:00:00

abstract：:To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...

journal_title：Cytotechnology

authors： Wu Z,Liu Y,Huang J,Huang Y,Fan L

更新日期：2019-06-26 00:00:00

abstract：:The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...

journal_title：Cytotechnology

authors： Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

更新日期：2015-05-01 00:00:00

abstract：:Fibroblast growth factor 21 (FGF21) potentially regulates glucose and lipid metabolism in energy homeostasis. We investigated dynamic changes in goat adipocytes treated with 75 nM FGF21 for 24, 36 and 48 h. Compared to controls, FGF21-treated adipocytes displayed smaller lipid droplets and altered levels of the mRNA t...

journal_title：Cytotechnology

authors： Zhang Y,Li L,Wang Q,Zhan S,Wang L,Zhong T,Guo J,Zhang H

更新日期：2018-10-01 00:00:00

abstract：:The metabolism of hybridoma cells was controlled to reduce metabolic formation in fed-batch cultures by dynamically feeding a salt-free nutrient concentrate. For this purpose, on-line oxygen uptake rate (OUR) measurement was used to estimate the metabolic demand of hybridoma cells and to determine the feeding rate of ...

journal_title：Cytotechnology

authors： Zhou W,Rehm J,Europa A,Hu WS

更新日期：1997-07-01 00:00:00

abstract：:The statistics of estimators used with the endpoint assay for virus titration were investigated. For a standard assay with 10 wells/dilution, the graphical estimator traditionally used was found to produce estimates with significant positive bias and a relatively low accuracy. Furthermore, the graphical estimator was ...

journal_title：Cytotechnology

authors： Nielsen LK,Smyth GK,Greenfield PF

更新日期：1992-01-01 00:00:00

abstract：:To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...

journal_title：Cytotechnology

authors： Zhou Y,Yi Y,Zhang Z,He J,Zhang Y

更新日期：2003-01-01 00:00:00

abstract：:There may exist a connection between Echinococcus granulosus infection and cancer development. Here, it is aimed to investigate specific effects of E. granulosus protoscoleces (PSCs) on the proliferation and invasion capacities of hepatocellular carcinoma (HCC) cells in vitro and ex vitro. HepG2 cells were cultured wi...

journal_title：Cytotechnology

authors： Yasen A,Wang M,Ran B,Lv G,Aji T,Xiao H,Shao Y,Wen H

更新日期：2021-02-01 00:00:00

abstract：:A Tubular Liquid Film Reactor was designed as a model system to transfer a batch culture kinetic to a continuous cascade. Cell density, product formation and substrate consumption rates were followed during fermentation at two dilution rates. In spite of the high dilution rates effective in each segment by itself high...

journal_title：Cytotechnology

authors： Borth N,Steindl F,Weigang F,Reiter M,Katinger H

更新日期：1990-05-01 00:00:00

abstract：:Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...

journal_title：Cytotechnology

authors： Dua R,Ramaswamy S

更新日期：2013-08-01 00:00:00

abstract：:A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. C...

journal_title：Cytotechnology

authors： Nakamichi N,Noda A,Yonezu T,Koike K,Matsumura T

更新日期：1997-11-01 00:00:00

abstract：:Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...

journal_title：Cytotechnology

authors： Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

更新日期：1997-01-01 00:00:00

abstract：:The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...

journal_title：Cytotechnology

authors： Webb E,Tkalcevic J,Edwards S,Hocking D,Nisbet I

更新日期：1996-01-01 00:00:00

abstract：:We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...

journal_title：Cytotechnology

authors： Akimaru K,Kuo MT,Furuta K,Suzuki M,Noyori R,Ishikawa T

更新日期：1996-01-01 00:00:00

abstract：:We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-beta) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-beta activity increases approximately 25-100% when the mouse EC cell line, F9, is ...

journal_title：Cytotechnology

authors： Kelly D,Campbell WJ,Tiesman J,Rizzino A

更新日期：1990-11-01 00:00:00

abstract：:Cisplatin is one of the most effective chemotherapeutic agents but injury may occur at higher doses. The aim of this study was to investigate the effect of bilberry on cisplatin induced toxic effects in rat ovary. Twenty-one female Wistar-Albino rats were utilized to form three groups: In group 1 (control group), each...

journal_title：Cytotechnology

authors： Pandir D,Kara O,Kara M

更新日期：2014-08-01 00:00:00

abstract：:The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...

journal_title：Cytotechnology

authors： Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

更新日期：2008-09-01 00:00:00

abstract：:Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell culture techniques, and in vivo by usi...

journal_title：Cytotechnology

authors： Kumalasari ID,Harmayani E,Lestari LA,Raharjo S,Asmara W,Nishi K,Sugahara T

更新日期：2012-03-01 00:00:00

abstract：:To explore the role of ribosomal protein S15A (RPS15A) in breast cancer. The Oncomine database was used to compare the expression of RPS15A in human breast cancer tissues and normal tissues. RPS15A in breast cancer cell line ZR-75-30 and BT474 was specifically knocked down using lentivirus-mediated short hairpin RNAs ...

journal_title：Cytotechnology

authors： Feng W,Liang C,Wang C,Yu X,Li Q,Yang H

更新日期：2018-10-01 00:00:00

abstract：:TNFalpha plays a crucial role in the pathogenesis of rheumatoid arthritis. It is very important to examine the expression of the TNF receptors, the ligand of TNFalpha. In this study, we developed a triple-color flow cytometric analysis using CD45 and CD14 monoclonal antibodies to simply detect the expression of the TN...

journal_title：Cytotechnology

authors： Ida H,Aramaki T,Nakamura H,Fujikawa K,Arima K,Tamai M,Kamachi M,Satoh K,Origuchi T,Kawakami A,Furuichi I,Kawabe Y,Eguchi K

更新日期：2009-07-01 00:00:00