Abstract：
:Animal cells in suspension culture can suffer severe mechanical damage from bursting gas bubbles or other hydrodynamic force sources. Certain chemical additives in the culture media, particularly some surface-active chemicals, can effectively protect animal cells against such damage. Previously we proposed that the protective effect is associated with the adsorption of the additives in the cell membrane through hydrophobic binding of the surface-active molecules to the membrane. Adsorption of the additives to the cell membrane may lead to decreased hydrophobicity of the cell surface, thus eliminating cell adhesion to bubbles and reducing cell damage from bursting bubbles. In this study, we measured the hydrophobicity of two insect cell lines based on cell adhesion to hydrocarbon phase and its influence by surface-active chemicals, Pluronic F68, a methylcellulose and a polyethylene glycol. The experimental results showed strong support for the aforecited cell protection mechanism.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Wu Jdoi
10.1007/BF00353929subject
Has Abstractpub_date
1996-01-01 00:00:00pages
103-9issue
1-3eissn
0920-9069issn
1573-0778journal_volume
22pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008021428969
更新日期：1998-11-01 00:00:00
abstract：:Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla e...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0173-5
更新日期：2018-04-01 00:00:00
abstract：:Anin vitro system is described which uses human diploid cells derived from the periodontal ligament. It simulates the clinical situation and is suitable for rapid cytotoxicity screening of dental materials. It can also be adapted to study various factors influencing biocompatibility. ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00146819
更新日期：1988-02-01 00:00:00
abstract：:We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-008-9135-2
更新日期：2008-02-01 00:00:00
abstract：:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-006-9002-y
更新日期：2006-03-01 00:00:00
abstract：:We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 w...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1014060113186
更新日期：2001-07-01 00:00:00
abstract：:Perilla frutescens is an Asian dietary herb consumed as an essential seasoning in Japanese cuisine as well as used for a Chinese medicine. Here, we report that a newly found methoxyflavanone derivative from P. frutescens (Perilla-derived methoxyflavanone, PDMF; 8-hydroxy-5,7-dimethoxyflavanone) shows carcinostatic act...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0116-1
更新日期：2018-06-01 00:00:00
abstract：:In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture co...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00365414
更新日期：1989-02-01 00:00:00
abstract：:A murine hybridoma line (Zac3), secreting an IgA monoclonal antibody, was cultivated in different systems: a BALB/c mouse, a T-flask, a stirred-tank bioreactor and a hollow fiber reactor. These systems were characterized in terms of cell metabolism and performances for IgA production. Cultures in T-flask and batch bio...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00749221
更新日期：1995-02-01 00:00:00
abstract：:Recent evidence from molecular cloning, biochemical and immunological experiments has established that the cation-independent mannose-6-phosphate (Man-6-P) receptor and insulin-like growth factor-II (IGF-II) receptor are the same protein. Although the role of the IGF-II/Man-6-P receptor as a transporter of hydrolytic ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00364994
更新日期：1989-12-01 00:00:00
abstract：:Human skin has continuous self-renewal potential throughout adult life and serves as first line of defence. Its cellular components such as human epidermal keratinocytes (HEKs) and dermal mesenchymal stromal cells (DMSCs) are valuable resources for wound healing applications and cell based therapies. Here we show a si...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-015-9857-x
更新日期：2016-08-01 00:00:00
abstract：:Benfuracarb is a carbamate insecticide used to control insect pests in vegetables and it has anti-acetylcholinesterase activity lower than other carbamates. Cytotoxic effects of benfuracarb were evaluated by using root growth inhibition (EC50), mitotic index (MI), and mitotic phase determinations on the root meristem ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9811-3
更新日期：2016-08-01 00:00:00
abstract：:Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008058031511
更新日期：1998-09-01 00:00:00
abstract：:The feasibility of using genipin cross-linked type II collagen scaffold with rabbit bone marrow mesenchymal stem cells (RBMSCs) to repair cartilage defect was herein studied. Induction of RBMSCs into chondrocytic phenotype on type II collagen scaffold in vitro was conducted using TGF-β 3 containing medium. After 3-wee...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-010-9314-9
更新日期：2011-01-01 00:00:00
abstract：:Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-016-0003-1
更新日期：2016-10-01 00:00:00
abstract：:Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of chromosome 21 could alter growth regulation and biochemical mechanisms, so we...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-010-9319-4
更新日期：2011-01-01 00:00:00
abstract：:Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008060720608
更新日期：1998-09-01 00:00:00
abstract：:Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in micros...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-012-9450-5
更新日期：2012-12-01 00:00:00
abstract：:Megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. However, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. In this study, a new fibroblast-like cell line was established from a...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9642-7
更新日期：2014-12-01 00:00:00
abstract：:In cartilage tissue engineering, viable cell numbers should be correctly counted in the collagenase digest of the biopsied cartilage. However, this is a difficult task due to the presence of matrix debris, cell ghosts and their aggregates. To search for the correct cell counting method in this situation, we evaluated ...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-010-9304-y
更新日期：2010-12-01 00:00:00
abstract：:This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques.Applications concern the setting of uptake-effect relationships a...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00746046
更新日期：1993-01-01 00:00:00
abstract：:A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00762410
更新日期：1994-01-01 00:00:00
abstract：:In recent years, water pollution has been converted to a challenging discussion in health area of human being. Heavy elements are one of the most important water pollutants and their negative adverse effects on body systems have been confirmed. In this study, investigation of effects of two heavy elements including le...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-013-9626-7
更新日期：2014-10-01 00:00:00
abstract：:Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/BF00386140
更新日期：1989-05-01 00:00:00
abstract：:Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a poly...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-014-9732-1
更新日期：2015-12-01 00:00:00
abstract：:In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-020-00419-2
更新日期：2020-10-01 00:00:00
abstract：:To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-019-00324-3
更新日期：2019-06-26 00:00:00
abstract：:The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized pro...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1007/s10616-017-0081-8
更新日期：2017-10-01 00:00:00
abstract：:ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...
journal_title：Cytotechnology
pub_type： 杂志文章
doi：10.1023/A:1008175226819
更新日期：2000-07-01 00:00:00
