Osteogenic differentiation of human placenta-derived mesenchymal stem cells (PMSCs) on electrospun nanofiber meshes.

Abstract:

:Numerous challenges remain in the successful clinical translation of cell-based therapeutic studies for skeletal tissue repair, including appropriate cell sources and viable cell delivery systems. Poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) amphiphilic block copolymers have been extensively explored in microspheres preparation. Due to the introduction of hydrophilic PEG segments into PCL backbones, these copolymers have shown much more potentials in carrying protein, lipophilic drugs or genes than commonly used poly (ε-caprolactone) (PCL) and poly (lactic acid). The aim of this study is to investigate the attachment and osteogenic differentiation of human placenta derived mesenchymal stem cells (PMSCs) on PEG-PCL triblock copolymers nanofiber scaffolds. Here we demonstrated that PMSCs proliferate robustly and can be effectively differentiated into osteogenic-like cells on nanofiber scaffolds. This study provides evidence for the use of nanofiber scaffolds as an ideal supporting material for in vitro PMSCs culture and an in vivo cell delivery vehicle for bone repair.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Zhang D,Tong A,Zhou L,Fang F,Guo G

doi

10.1007/s10616-012-9450-5

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

701-10

issue

6

eissn

0920-9069

issn

1573-0778

journal_volume

64

pub_type

杂志文章
  • Development and characterization of insect cell lines.

    abstract:CONCLUSIONS:With the wide availability of insect cell culture media, it can generally be considered a routine process to develop new cell lines. Exceptions to this statement do exist, of course. Difficulties may arise when attempting to culture a specific cell type. For example, while there are a few cell lines from in...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00350384

    authors: Lynn DE

    更新日期:1996-01-01 00:00:00

  • Morphological observation and analysis using automated image cytometry for the comparison of trypan blue and fluorescence-based viability detection method.

    abstract::The ability to accurately determine cell viability is essential to performing a well-controlled biological experiment. Typical experiments range from standard cell culturing to advanced cell-based assays that may require cell viability measurement for downstream experiments. The traditional cell viability measurement ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9704-5

    authors: Chan LL,Kuksin D,Laverty DJ,Saldi S,Qiu J

    更新日期:2015-05-01 00:00:00

  • Modified CelliGen-packed bed bioreactors for hybridoma cell cultures.

    abstract::This study describes two packed bed bioreactor configurations which were used to culture a mouse-mouse hybridoma cell line (ATCC HB-57) which produces an IgG1 monoclonal antibody. The first configuration consists of a packed column which is continuously perfused by recirculating oxygenated media through the column. In...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02521730

    authors: Wang G,Zhang W,Jacklin C,Freedman D,Eppstein L,Kadouri A

    更新日期:1992-01-01 00:00:00

  • DESI-MS as a tool for direct lipid analysis in cultured cells.

    abstract::Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface p...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-014-9734-z

    authors: Bodzon-Kulakowska A,Cichon T,Golec A,Drabik A,Ner J,Suder P

    更新日期:2015-12-01 00:00:00

  • Eicosapentaenoic acid protects against 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced hepatic toxicity in cultured rat hepatocytes.

    abstract::2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent and ubiquitous environmental contaminant. The health impact of TCDD exposure is of great concern to the general public. Recent reports have implied that eicosapentaenoic acid (EPA) might be a potential chemopreventive agent and influence hepatotoxicity. The ai...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-011-9386-1

    authors: Turkez H,Geyikoglu F,Mokhtar YI,Togar B

    更新日期:2012-01-01 00:00:00

  • Evaluation of the antiviral effect of new compounds by using cellular cultures.

    abstract::Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00736794

    authors: Dianzani F,Antonelli G

    更新日期:1991-02-01 00:00:00

  • The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway.

    abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-016-9995-9

    authors: Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

    更新日期:2016-12-01 00:00:00

  • Expression of anchorin CII mRNA by cultured chondrocytes.

    abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00736804

    authors: Castagnola P,Cancedda R

    更新日期:1991-02-01 00:00:00

  • A plant extract which enhances the plating efficiency of lymphoid cell lines and enhances the survival of normal lymphoid cells in vitro.

    abstract::A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enh...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00772193

    authors: Dunstan CA,Andersson J,Bohlin L,Cox PA,Grönvik KO

    更新日期:1994-01-01 00:00:00

  • MiR-548c-3p inhibits the proliferation, migration and invasion of human breast cancer cell by targeting E2F3.

    abstract::MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00418-3

    authors: Tan PY,Wen LJ,Li HN,Chai SW

    更新日期:2020-10-01 00:00:00

  • Cell cycle, cell size and mitochondrial activity of hybridoma cells during batch cultivation.

    abstract::Cell cycle, cell size and rhodamine 123 fluorescence in cell populations of two batch cultures were analysed and quantified with a fluorescence-activated cell sorter (FACS). Two cultures derived from either exponential or stationary phase innocula were investigated in order to demonstrate the dependency of the subsequ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00365929

    authors: al-Rubeai M,Chalder S,Bird R,Emery AN

    更新日期:1991-11-01 00:00:00

  • Septin 6 localizes to microtubules in neuronal dendrites.

    abstract::In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-012-9477-7

    authors: Moon IS,Lee H,Walikonis RS

    更新日期:2013-03-01 00:00:00

  • The organotypic culture of human skin keratinocytes and fibroblasts to achieve form and function.

    abstract::We describe an organotypic model of human skin comprised of a stratified layer of human epidermal keratinocytes and dermal fibroblasts within a contracted collagen lattice. Feasible and reproducible production of the skin construct has required the use of traditional as well as specialized culture techniques. The conf...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02521744

    authors: Parenteau NL,Bilbo P,Nolte CJ,Mason VS,Rosenberg M

    更新日期:1992-01-01 00:00:00

  • Improved reproducibility in preparing precision-cut liver tissue slices.

    abstract::Precision-cut liver tissue slices (PCLS) have been used for decades to study pharmacological metabolism as well as toxicology and efficacy of novel substances on primary material under standardized conditions. Slicing of primary liver tissue has been done using different slicing machines. Since there has been great va...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-009-9246-4

    authors: Zimmermann M,Lampe J,Lange S,Smirnow I,Königsrainer A,Hann-von-Weyhern C,Fend F,Gregor M,Bitzer M,Lauer UM

    更新日期:2009-12-01 00:00:00

  • DNA and cell death.

    abstract::The type of DNA damage and the role of poly (ADP-ribosyl) polymerase (ADPRP) and sulphated glyprotein 2 (SGP-2) in programmed cell death (apoptosis) was investigated in the following model systems: i) rat thymocytes treated with dexamethasone (DEX) eitherin vitro orin vivo; ii) human perypheral blood mononuclear cells...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00736814

    authors: Monti D,Troiano L,Tropea F,Grassilli E,Cossarizza A,Barozzi D,Bettuzzi S,Corti A,Franceschi C

    更新日期:1991-02-01 00:00:00

  • Regulation of hTERT transcription: a target of cellular and viral mechanisms for immortalization and carcinogenesis.

    abstract::A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-004-5122-4

    authors: Horikawa I,Michishita E,Barrett JC

    更新日期:2004-06-01 00:00:00

  • Modified method for effective primary vascular smooth muscle progenitor cell culture from peripheral blood.

    abstract::In previous studies, vascular smooth muscle progenitor cells (vSMPCs) isolated from peripheral blood mononuclear cells (PBMCs) were cultured using medium containing platelet-derived growth factor-BB (PDGF-BB) for 4 weeks. However, this method requires long culture periods of up to 4 weeks and yields low cell counts. T...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-020-00419-2

    authors: Seong JH,Song YS,Joo HW,Park IH,Shen GY,Shin NK,Lee AH,Kwon AM,Lee Y,Kim H,Kim KS

    更新日期:2020-10-01 00:00:00

  • Change in growth kinetics of hybridoma cells entrapped in collagen gel affected by alkaline supply.

    abstract::The growth yields for glucose and glutamine of murine hybridoma cells entrapped in collagen gel particles were examined during the growth phase. The immobilized hybridoma cells were cultivated in a fluidized bed fermenter where the medium was circulating to supply oxygen separately. Procedures to supply an alkaline so...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00758178

    authors: Shirai Y,Yamaguchi M,Kobayashi A,Nishi A,Nakamura H,Murakami H

    更新日期:1994-01-01 00:00:00

  • Differential expression of mRNAs encoding laminin chain variants during in vitro development of mouse blastocysts.

    abstract::Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008084508489

    authors: Azimi M,Niimi T,Yoshida N,Kitagawa Y

    更新日期:1999-09-01 00:00:00

  • Features of free software packages in flow cytometry: a comparison between four non-commercial software sources.

    abstract::Flow cytometers designed to analyze large particles are enabling new applications in biology. Data analysis is a critical component of the process FCM. In this article we compare features of four free software packages including WinMDI, Cyflogic, Flowing software, and Cytobank. ...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9609-8

    authors: Sahraneshin Samani F,Moore JK,Khosravani P,Ebrahimi M

    更新日期:2014-08-01 00:00:00

  • Erythropoietin processing in erythropoietic system and central nervous system.

    abstract::We describe possible functions of carbohydrates attached to growth factors and strategies to examine the functions, concentrating on erythropoietin, a major regulator of erythropoiesis. Erythropoietin in erythropoiesis functions as an endocrine hormone; it is produced by kidney cells and transferred into the circulati...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00744323

    authors: Nagao M,Masuda S,Ueda M,Sasaki R

    更新日期:1995-01-01 00:00:00

  • Zoledronic acid suppresses metastasis of esophageal squamous cell carcinoma cells through upregulating the tight junction protein occludin.

    abstract::We have previously demonstrated the radio-sensitizing effect of zoledronic acid (ZOL), a third generation bisphosphonate, on human esophageal squamous cell carcinoma (ESCC) cells. Here we show that ZOL suppresses metastatic progression of ESCC cells mainly through up-regulating the tight junction protein occludin. Exp...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-015-9884-7

    authors: Lin C,Xin S,Qin X,Li H,Lin L,You Y

    更新日期:2016-08-01 00:00:00

  • Endogenous synthesis of prostacyclin was positively regulated during the maturation phase of cultured adipocytes.

    abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9616-9

    authors: Rahman MS,Khan F,Syeda PK,Nishimura K,Jisaka M,Nagaya T,Shono F,Yokota K

    更新日期:2014-08-01 00:00:00

  • Metabolic rates of vascular endothelial cellsin vitro.

    abstract::Cultures of endothelial cells and cell lines of endothelial origin were maintained at confluence without medium exchange for a period of 72 h. During this time period the concentration of nutrients - amino acids and glucose - and metabolic waste products - lactate and ammonium - was determined as well as cell vitality...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/BF00761778

    authors: Schrimpf G,Schröder M,Weitnauer E,Friedl P

    更新日期:1994-01-01 00:00:00

  • Uptake and efflux of FL118 and two FL118 derivatives in 3D cell model.

    abstract::Drug uptake and efflux are two of the critical factors required in order to be able to define drug efficacy. This study aims to investigate cytotoxicity and uptake mechanisms of two FL118 analogues (7-Q20 and val-FL118) in parallel with FL118 in three-dimensional multi-cellular spheroids model. The influence of compou...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00322-5

    authors: Zhou L,Weng Q,Zheng Y,Zhou Y,Li Q,Li F

    更新日期:2019-07-12 00:00:00

  • Purification and characterization of alpha-D-galactosidase produced by ADG cell line established from abalon digestive gland.

    abstract::ADG cell line was established from an abalonedigestive gland and previously characterized. ADGcells have the potential to grow in protein-freeculture and secrete l3 types of glycosidases. Inthis article, we determined the origin of ADG cell line,using electron microscopy, and purified a glycosidasesecreted by these ce...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1023/A:1008175226819

    authors: Kusumoto K,Shirahata S,Kamei Y

    更新日期:2000-07-01 00:00:00

  • Comparative cytogenetics between two species of the family Pseudopimelodidae (Siluriformes): occurrence of natural triploidy and supernumerary chromosomes.

    abstract::The Pseudopimelodidae family comprises 35 species however, cytogenetic studies have been performed in only six species. This study uncovered karyotypic data on Pseudopimelodus pulcher and Microglanis cottoides. Both species possessed 2n = 54, with 20m + 16sm + 10st + 8a and FN = 100 for P. pulcher and 30m + 14sm + 6st...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-013-9676-x

    authors: Gouveia JG,de Moraes VP,Pires LB,da Rosa R,Dias AL

    更新日期:2015-03-01 00:00:00

  • Small interfering RNA knockdown of mini-TyrRS and mini-TrpRS effects angiogenesis in human umbilical vein endothelial cells in hypoxic culture.

    abstract::Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-ti...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-008-9151-2

    authors: Zeng R,Chen YC,Zeng Z,Liu R,Qiang O,Jiang XF,Liu XX,Li X,Wang HY

    更新日期:2008-03-01 00:00:00

  • Pig testis extract augments adiponectin expression and secretion through the peroxisome proliferator-activated receptor signaling pathway in 3T3-L1 adipocytes.

    abstract::Adiponectin is a key molecule whose upregulation may ameliorate symptoms of type 2 diabetes mellitus and disorders of lipid metabolism. Several plant-derived components have been shown to enhance adiponectin secretion; however, there have been no reports on the effects of animal-derived products. Therefore, in the cur...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-018-0213-9

    authors: Kadooka K,Sato M,Matsumoto T,Kuhara S,Katakura Y,Fujimura T

    更新日期:2018-06-01 00:00:00

  • Synthesis, characterization and cytotoxicity of boron nitride nanoparticles: emphasis on toxicogenomics.

    abstract::Nanotechnology is increasingly developing area including more than 700 commercial products such as clothing, food preparation, cosmetics, mechanics, electronics and also health industry. People generally contact with nanoparticles by inhaling from air. Thus, it is becoming important issue to understand harmful effects...

    journal_title:Cytotechnology

    pub_type: 杂志文章

    doi:10.1007/s10616-019-00292-8

    authors: Türkez H,Arslan ME,Sönmez E,Açikyildiz M,Tatar A,Geyikoğlu F

    更新日期:2019-02-01 00:00:00