Abstract:
:We reported previously that adenocarcinoma-reactive human monoclonal antibody AE6F4, which had been generated by in vitro immunization method, recognizes both 14-3-3protein and cytokeratin 8 (CK8). In this study, to analyze the cross-reactivity of AE6F4 antibody, epitopes of AE6F4 antibody on 14-3-3 proteins and CK8 were studied by using synthetic linear peptide scanning technology. To determine the locations of B cell epitope, 48 and 95 of decapeptides covering the entire 14-3-3 proteins and CK8, respectively,were synthesized and binding to AE6F4 antibody was examined by ELISA. The AE6F4 antibody was strongly reactive to peptides containing amino acid sequences TLWTSDTQGD in 14-3-3 proteins and INFLRQLYEE in CK8. These results indicate that AE6F4 antibody can recognize the different peptide sequences in 14-3-3 proteins and CK8.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Ichikawa A,Shim SY,Katakura Y,Teruya K,Hashizume S,Shirahata Sdoi
10.1023/A:1014060113186subject
Has Abstractpub_date
2001-07-01 00:00:00pages
101-7issue
1-3eissn
0920-9069issn
1573-0778journal_volume
36pub_type
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