The role of recombinant proteins in the development of serum-free media.

Abstract:

:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived. Given recent concerns about TSEs (transmissible spongiform encephalopathies) and other adventitious agents, the drive in media formulations must be towards elimination of animal-origin materials while maintaining cell line productivity. The progress made towards removing animal-derived components and the use of recombinant proteins in serum-free media for mammalian cells is reviewed.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Keenan J,Pearson D,Clynes M

doi

10.1007/s10616-006-9002-y

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

49-56

issue

1-3

eissn

0920-9069

issn

1573-0778

journal_volume

50

pub_type

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