Abstract:
:Early developments in serum-free media led to a variety of formulations in which components normally provided in serum and required for growth (insulin, transferrin, lipid supplements, trace elements) and poorly defined components (extracts, hydrolysates) were added to defined basal media. These additives were mostly animal-derived. Given recent concerns about TSEs (transmissible spongiform encephalopathies) and other adventitious agents, the drive in media formulations must be towards elimination of animal-origin materials while maintaining cell line productivity. The progress made towards removing animal-derived components and the use of recombinant proteins in serum-free media for mammalian cells is reviewed.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Keenan J,Pearson D,Clynes Mdoi
10.1007/s10616-006-9002-ysubject
Has Abstractpub_date
2006-03-01 00:00:00pages
49-56issue
1-3eissn
0920-9069issn
1573-0778journal_volume
50pub_type
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