Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells.

abstract：:Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...

journal_title：Cytotechnology

authors： Marques CL,Rafael MS,Cancela ML,Laizé V

更新日期：2007-09-01 00:00:00

abstract：:This work focused on determining the effect of dissolved oxygen concentration (DO) on growth and metabolism of BHK-21 cell line (host cell for recombinant proteins manufacturing and viral vaccines) cultured in two stirred tank bioreactors with different aeration-homogenization systems, as well as pH control mode. BHK-...

journal_title：Cytotechnology

authors： Núñez EG,Leme J,de Almeida Parizotto L,Chagas WA,de Rezende AG,da Costa BL,Monteiro DC,Boldorini VL,Jorge SA,Astray RM,Pereira CA,Caricati CP,Tonso A

更新日期：2014-08-01 00:00:00

abstract：:A silicon microsensor (ISFET - Ion Sensitive Field Effect Transistor) has been used to detect the metabolism of a cell population cultured on a coverslip and positioned close to the sensor surface. The system output is analyzed as a function of cell density. ...

journal_title：Cytotechnology

authors： Martinoia S,Meloni M,Parodi MT,Tedesco M,Ciccarelli C,Grattarola M

更新日期：1993-01-01 00:00:00

abstract：:Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

journal_title：Cytotechnology

authors： Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

更新日期：2017-12-01 00:00:00

abstract：:In the last 10 years, new assignments and the special demands of mammalian cells to the culture conditions caused the development of complex small scale fermentation setups. The use of continuous fermentation and cell retention devices requires appropriate process control systems. An arrangement for control and data-a...

journal_title：Cytotechnology

authors： Büntemeyer H,Marzahl R,Lehmann J

更新日期：1994-01-01 00:00:00

abstract：:Models of cell processes can be particularly useful in simulating, optimizing and controlling cell culture systems. Models reported in the literature are of various degrees of biological structure and mathematical complexity and describe cell growth, death, metabolism, and product formation, alone or in combination wi...

journal_title：Cytotechnology

authors： Tziampazis E,Sambanis A

更新日期：1994-01-01 00:00:00

abstract：:Glioblastomas (GBM) are aggressive brain tumors with very poor prognosis. While silver nanoparticles represent a potential new strategy for anticancer therapy, the silver/silver chloride nanoparticles (Ag/AgCl-NPs) have microbicidal activity, but had not been tested against tumor cells. Here, we analyzed the effect of...

journal_title：Cytotechnology

authors： Eugenio M,Campanati L,Müller N,Romão LF,de Souza J,Alves-Leon S,de Souza W,Sant'Anna C

更新日期：2018-12-01 00:00:00

abstract：CONCLUSIONS:The BEVS has become widely utilized for production of recombinant proteins. However, protein aggregation and inefficient processing often limit yields, especially for secreted and membrane proteins. Since many proteins of pharmaceutical interest require similar posttranslational processing steps, engineerin...

journal_title：Cytotechnology

authors： Betenbaugh MJ,Ailor E,Whiteley E,Hinderliter P,Hsu TA

更新日期：1996-01-01 00:00:00

abstract：:Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology an...

journal_title：Cytotechnology

authors： Cheng S,Yang X,Zhang Y,Xiao C

更新日期：2013-08-01 00:00:00

abstract：:As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...

journal_title：Cytotechnology

authors： Beas-Catena A,Sánchez-Mirón A,García-Camacho F,Molina-Grima E

更新日期：2011-12-01 00:00:00

abstract：:This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected w...

journal_title：Cytotechnology

authors： Kuhlmann I

更新日期：1995-06-01 00:00:00

abstract：:Statins may have beneficial effects in atherogenesis given their antithrombotic properties involving non-lipid mechanisms that modify endothelial function of tissue factor induction by thrombin. In this study, we investigate the effect of atorvastatin on tissue factor (TF) activity in thrombin-stimulated endothelial c...

journal_title：Cytotechnology

authors： Martínez-Sales V,Vila V,Ferrando M,Reganon E

更新日期：2011-01-01 00:00:00

abstract：:We modified an existing protocol to develop a more efficient method to acquire and culture muscle-derived stem cells (MDSCs) and compared the characteristics of cells obtained from the two methods. This method is based on currently used multistep enzymatic digestion and preplate technique. During the replating process...

journal_title：Cytotechnology

authors： Xu Z,Yu L,Lu H,Feng W,Chen L,Zhou J,Yang X,Qi Z

更新日期：2018-12-01 00:00:00

abstract：:Various antitumor agents induce apoptotic cell death in tumor cells. Since the apoptosis program in tumor cells plays a critical role in the chemotherapy-induced tumor cell killing, it is suggested that the defect in the signaling pathway of apoptosis could cause a new form of multidrug resistance in tumor cells. This...

journal_title：Cytotechnology

authors： Mashima T,Seimiya H,Chen Z,Kataoka S,Tsuruo T

更新日期：1998-09-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00

abstract：:It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the pres...

journal_title：Cytotechnology

authors： Zhang X,Lan W,Deng Y,Ma Y,Liu K,Peng J,Li Y,Hong H

更新日期：2008-07-01 00:00:00

abstract：:In the recent years, the possibility of utilizing extracellular vesicles for drug delivery purposes has been investigated in various models, suggesting that these vesicles may have such potential. In addition to the choice of donor cell type for vesicle production, a major obstacle still exists with respect of loading...

journal_title：Cytotechnology

authors： Johnsen KB,Gudbergsson JM,Skov MN,Christiansen G,Gurevich L,Moos T,Duroux M

更新日期：2016-10-01 00:00:00

abstract：:Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...

journal_title：Cytotechnology

authors： Sununliganon L,Singhatanadgit W

更新日期：2012-01-01 00:00:00

abstract：:The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...

journal_title：Cytotechnology

authors： Castagnola P,Cancedda R

更新日期：1991-02-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

journal_title：Cytotechnology

authors： Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

更新日期：2016-12-01 00:00:00

abstract：:In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the firs...

journal_title：Cytotechnology

authors： Celik M,Unal F,Yüzbaşıoğlu D,Yılmaz S,Aksoy H,Karaman S

更新日期：2006-06-01 00:00:00

abstract：:Achyranthes bidentata (A. bidentata) Blume is a medicinal herb with the property of strengthening bones and muscles and ensuring proper downward flow of blood in terms of the therapeutic theory of traditional medicine. In the present study, the effect of A. bidentata root extract (AE) on osteoblast function was invest...

journal_title：Cytotechnology

authors： Suh KS,Lee YS,Choi EM

更新日期：2014-12-01 00:00:00

abstract：:Therapeutic antibody IgG1 has two N-linked oligosaccharide chains bound to the Fc region. The oligosaccharides are of the complex biantennary type, composed of a trimannosyl core structure with the presence or absence of core fucose, bisecting N-acetylglucosamine (GlcNAc), galactose, and terminal sialic acid, which gi...

journal_title：Cytotechnology

authors： Mori K,Iida S,Yamane-Ohnuki N,Kanda Y,Kuni-Kamochi R,Nakano R,Imai-Nishiya H,Okazaki A,Shinkawa T,Natsume A,Niwa R,Shitara K,Satoh M

更新日期：2007-12-01 00:00:00

abstract：:Cells transfected by retroviral vectors are brought in agene of particular interest and are very useful in avariety of experiments. It is essential to testify that theDNA fragment was successfully introduced into the cellstogether with the retroviral vectors. Polymerase chainreaction is believed to be a fast and conve...

journal_title：Cytotechnology

authors： Ding LH,Iimura E,Saijo K,Hamada H,Ohno T

更新日期：2000-11-01 00:00:00

abstract：:High density cell culture systems offer the advantage of production of bio-pharmaceuticals in compact bioreactors with high volumetric production rates; however, these systems are difficult to design and operate. First of all, the cells have to be retained in the bioreactor by physical means during perfusion. The desi...

journal_title：Cytotechnology

authors： Ozturk SS

更新日期：1996-01-01 00:00:00

abstract：:More than 35 years ago double Nobel laureate Linus Carl Pauling published a powerful model of the molecular mechanism of general anesthesia, generally referred to as the hydrate-microcrystal (aqueous-phase) theory. This hypothesis, based on the molecular behavior of water molecules, did not receive serious attention d...

journal_title：Cytotechnology

authors： Nakada T

更新日期：2009-04-01 00:00:00

abstract：:A fructose-based cell culture is suitable for the process control of protein production because of slow sugar consumption rate and low lactate accumulation. The fructose transporter, GLUT5, mediates its incorporation into cells and is required for the fructose-based culture. In order to produce efficiently recombinant...

journal_title：Cytotechnology

authors： Inoue Y,Tsukamoto Y,Yamanaka M,Nakamura S,Inoue A,Nishino N,Kawahara H

更新日期：2010-08-01 00:00:00

abstract：:In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to ree...

journal_title：Cytotechnology

authors： Fernyhough ME,Vierck JL,Hausman GJ,Mir PS,Okine EK,Dodson MV

更新日期：2004-10-01 00:00:00

abstract：:The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncated heavy and light chains. The light chain was very efficiently secret...

journal_title：Cytotechnology

authors： Webb E,Tkalcevic J,Edwards S,Hocking D,Nisbet I

更新日期：1996-01-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00