Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization.

Abstract:

:The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Tanaka H,Fukuda N,Shoyama Y

doi

10.1023/A:1008068718392

subject

Has Abstract

pub_date

1999-03-01 00:00:00

pages

115-20

issue

2

eissn

0920-9069

issn

1573-0778

journal_volume

29

pub_type

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