Formation of multilayer rosettes in phytohemagglutinin-stimulated lymphocytes: correlation with chromatin condensation conformation in premature chromosome condensation.

Abstract:

:We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formation suggests that changes in chromatin are a prerequisite for rosette formation. Rosette formation was most pronounced for 24-h and 48-h cultures. Chromatin decondensation and rosette formation showed identical patterns. The possibility that multilayer rosette formation is directly dependent on conformational changes of chromatin is discussed.

journal_name

Cytotechnology

journal_title

Cytotechnology

authors

Thomou H,Sambani C,Kitsiou P,Politis G

doi

10.1007/BF00145027

subject

Has Abstract

pub_date

1988-07-01 00:00:00

pages

243-9

issue

3

eissn

0920-9069

issn

1573-0778

journal_volume

1

pub_type

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