Abstract:
:We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formation suggests that changes in chromatin are a prerequisite for rosette formation. Rosette formation was most pronounced for 24-h and 48-h cultures. Chromatin decondensation and rosette formation showed identical patterns. The possibility that multilayer rosette formation is directly dependent on conformational changes of chromatin is discussed.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Thomou H,Sambani C,Kitsiou P,Politis Gdoi
10.1007/BF00145027subject
Has Abstractpub_date
1988-07-01 00:00:00pages
243-9issue
3eissn
0920-9069issn
1573-0778journal_volume
1pub_type
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