Succinoyl trehalose lipid induced differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophages.

abstract：:In neuronal dendrites, septins localize to the base of the spine, a unique position which is sandwiched between the microtubule (MT)-rich dendritic shaft and the actin filament-rich spine. Here, we provide evidence for the association of SEPT6 with MTs in cultured rat hippocampal neurons. In normal cultures, SEPT6 clu...

journal_title：Cytotechnology

authors： Moon IS,Lee H,Walikonis RS

更新日期：2013-03-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has been used for the general detection of Mollicutes. 25 Mycoplasma and Acholeplasma species were detected including important contaminants of cell cultures such as M. orale, M. arginini, M. hyorhinis, M. fermentans, A. laidlawii and additional human and animal mycoplasmas. PCR rea...

journal_title：Cytotechnology

authors： Wirth M,Berthold E,Grashoff M,Pfützner H,Schubert U,Hauser H

更新日期：1994-01-01 00:00:00

abstract：:Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite...

journal_title：Cytotechnology

authors： Bektaş İ,Karaman Ş,Dıraz E,Çelik M

更新日期：2016-12-01 00:00:00

abstract：:As chemical pesticides are being banned as control agents for agricultural pests, the use of the highly specific, safe to non-target organisms baculoviruses has been proposed. These viruses can be produced either in vivo or in vitro. In vitro production requires appropriated host insect cell lines with the ability for...

journal_title：Cytotechnology

authors： Beas-Catena A,Sánchez-Mirón A,García-Camacho F,Molina-Grima E

更新日期：2011-12-01 00:00:00

abstract：:A simple protein free medium was formulated and tested in suspension culture using three hybridoma cell lines. The medium, referred to as CDSS (Chemically Defined Serum Substitutes), consisted of the basal medium DMEM:Ham F12, 1:1, with HEPES (D12H), plus pluronic F68, trace elements, ferric citrate, ascorbic acid, an...

journal_title：Cytotechnology

authors： Qi YM,Greenfield PF,Reid S

更新日期：1996-06-01 00:00:00

abstract：:The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented w...

journal_title：Cytotechnology

authors： Sadeesh EM,Selokar NL,Balhara AK,Yadav PS

更新日期：2016-10-01 00:00:00

abstract：:Tea catechin is one of the compounds that are closely related to obesity and insulin sensitivity. In order to determine the effect of catechin on adipocyte differentiation, we treated 3T3-L1 preadipocytes with different kinds of catechins. Our results showed that catechins, especially epigallocatechin gallate (EGCG), ...

journal_title：Cytotechnology

authors： Kim H,Hiraishi A,Tsuchiya K,Sakamoto K

更新日期：2010-07-01 00:00:00

abstract：:We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent...

journal_title：Cytotechnology

authors： Schoofs G,Monica TJ,Ayala J,Horwitz J,Montgomery T,Roth G,Castillo FJ

更新日期：1998-11-01 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:A human-mouse hybridoma clone 92-2 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was established. To detect antibody-binding sequences on Ara h1, we synthesized a series of peptides of the Ara h1 protein on a multi-pin apparatus for the pin-peptide ELISA. The 92-2 human monoclonal ant...

journal_title：Cytotechnology

authors： Shinmoto H,Matsuo Y,Naganawa Y,Tomita S,Takano-Ishikawa Y

更新日期：2010-08-01 00:00:00

abstract：:Hypertension (HT) is a common and life threating health problem worldwide leading to stroke, heart attack and renal failure. It is characterized by elevated blood pressure forced heart load. Human interleukin-6 (IL-6) and C- reactive protein (CRP) are known to be involved in inflammatory processes. IL-6 gene is a poly...

journal_title：Cytotechnology

authors： Karaman E,Urhan Kucuk M,Bayramoglu A,Uzun Göçmen S,Ercan S,Guler HI,Kucukkaya Y,Erden S

更新日期：2015-12-01 00:00:00

abstract：:This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with ...

journal_title：Cytotechnology

authors： Xu Y,Zhang J,Ma Y,Han Y,Min J,Liang Y,Zhao D,Qiu J

更新日期：2014-10-01 00:00:00

abstract：:The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...

journal_title：Cytotechnology

authors： Nakamura N,Aoki Y,Horiuchi H,Furusawa S,Yamanaka HI,Kitamoto T,Matsuda H

更新日期：2000-03-01 00:00:00

abstract：:Various parts of Mimusops elengi Linn. (Sapotaceae) have been used widely in traditional Indian medicine for the treatment of pain, inflammation and wounds. The study was conducted to explore the use of stem bark of M. elengi on pharmacological grounds and to evaluate the scientific basis of cytotoxic and anti-tumor a...

journal_title：Cytotechnology

authors： Kumar H,Savaliya M,Biswas S,Nayak PG,Maliyakkal N,Manjunath Setty M,Gourishetti K,Pai KS

更新日期：2016-08-01 00:00:00

abstract：:Vascular growth factors, including vascular endothelial growth factor and fibroblast growth factor-2, bind to heparan sulfate proteoglycans in the basement membrane. While this binding, storage, and release system provides a critical model for controlled drug release devices, basement membrane-growth factor binding ki...

journal_title：Cytotechnology

authors： Clyne AM,Edelman ER

更新日期：2009-07-01 00:00:00

abstract：:The potential of spent medium to support the growth and recombinant protein production of High-Fivetrade mark cells was investigated. Growth in medium consisting of three parts fresh and one part spent medium was comparable to that in fresh medium (maximal specific growth rates of 0.028 and 0.029 h(-1), and maximal ce...

journal_title：Cytotechnology

authors： Ikonomou L,Bastin G,Schneider YJ,Agathos SN

更新日期：2004-01-01 00:00:00

abstract：:Temperature reduction in CHO cell batch culture may be beneficial in the production of recombinant protein and in maintenance of viability. The effects on cell cycle, apoptosis and nucleotide pools were studied in cultures initiated at 37°C and temperature shifted to 30 °C after 48 hours. In control cultures maintaine...

journal_title：Cytotechnology

authors： Moore A,Mercer J,Dutina G,Donahue CJ,Bauer KD,Mather JP,Etcheverry T,Ryll T

更新日期：1997-01-01 00:00:00

abstract：:A differentiation method of human bone marrow mesenchymal stem cells (MSCs) to chondrocytes was developed for the construction of a three-dimensional (3D) cartilage tissue. The adhesive cells, which were isolated from a human bone marrow aspirate were embedded in type I collagen in a poly-L: -lactate-glycolic acid cop...

journal_title：Cytotechnology

authors： Matsuda C,Takagi M,Hattori T,Wakitani S,Yoshida T

更新日期：2005-01-01 00:00:00

abstract：:The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybri...

journal_title：Cytotechnology

authors： Iwamoto K,Shintani Y,Sasada R,Honjo T,Kitano K

更新日期：1990-07-01 00:00:00

abstract：:We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...

journal_title：Cytotechnology

authors： Kamei Y,Sagara A

更新日期：2002-11-01 00:00:00

abstract：:We have developed a generic transient transfection process at 100 L scale, using HEK293-EBNA cells and PEI as the transfection reagent for the production of recombinant IgG. The process, including large-scale plasmid preparation, expression at bioreactor scale, capture, purification and, if necessary, endotoxin remova...

journal_title：Cytotechnology

authors： Tuvesson O,Uhe C,Rozkov A,Lüllau E

更新日期：2008-02-01 00:00:00

abstract：:Olive oil intake has been shown to induce significant levels of apoptosis in various cancer cells. These anti-cancer properties are thought to be mediated by phenolic compounds present in olive. These beneficial health effects of olive have been attributed, at least in part, to the presence of oleuropein and hydroxyty...

journal_title：Cytotechnology

authors： Han J,Talorete TP,Yamada P,Isoda H

更新日期：2009-01-01 00:00:00

abstract：:A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...

journal_title：Cytotechnology

authors： Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

更新日期：1994-01-01 00:00:00

abstract：:A sequential multiprobe fluorescence in situ hybridization technique was developed to study the 13, 18, 21, X and Y chromatid segregation in human lymphocytes anaphases cultures without antimitotic treatment. This method was used to know if exist any different chromosomes segregation in lymphocytes from Down syndrome ...

journal_title：Cytotechnology

authors： Escudero T,Coll MD,Fuster C,Egozcue J

更新日期：2002-09-01 00:00:00

abstract：:MiR-548 has been reported to be involved in a variety of tumor processes, but its function in breast cancer remains unclear. In this study, we found that miR-548 was low expressed in breast cancer tissues and cells compared with normal control. We then examined whether up-regulation of miR-548 could improve the progre...

journal_title：Cytotechnology

authors： Tan PY,Wen LJ,Li HN,Chai SW

更新日期：2020-10-01 00:00:00

abstract：:Effective harvesting procedure for adipose tissue is demanded by the affordable Good Manufacturing Practice-Compliant Production of clinical-grade adipose tissue-derived stem cells (hADSCs). Enzymatic digestion using collagenase is the most reliable method of adipose tissue-derived stem cells (hADSCs) isolation, while...

journal_title：Cytotechnology

authors： Li Z,Mu D,Liu C,Xin M,Fu S,Li S,Qi J,Wang Q,Luan J

更新日期：2020-04-01 00:00:00

abstract：:Material and degree of reductance balance equations are used to estimate the rates of oxygen uptake and carbon dioxide evolution of animal cell cultures. Lumped compositions, molecular weight and reductance degree of cellular protein, monoclonal antibody, biomass and amino acid consumption (excluding glutamine and ala...

journal_title：Cytotechnology

authors： Xiu ZL,Deckwer WD,Zeng AP

更新日期：1999-05-01 00:00:00

abstract：:Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...

journal_title：Cytotechnology

authors： Feng X,Shen S,Cao P,Zhu L,Zhang Y,Zheng K,Feng G,Zhang D

更新日期：2016-12-01 00:00:00

abstract：:Endothelial cells are involved in important pathological situations. They could be the target for infectious processes as for example in Cowdriosis, an important disease in cattle due to the rickettsia Cowdria ruminantium prevalent in the south of the Sahara. They are also connected to angiogenic processes related to ...

journal_title：Cytotechnology

authors： Marique T,Blankaert D,Hendrick V,Raschella A,Declerck B,Alloin C,Teixera-Guerra I,Sandron D,Cherlet M,Parent D,Kirkpatrick C,Van Vooren JP,Wérenne J

更新日期：1997-11-01 00:00:00

abstract：:The cytotoxic necrotizing factor type 1 (CNF1) fromE. coli causes necrosis in rabbit skin and multinucleation in cultured cells. Cells exposed to CNF1 were characterized by changes in actin organization, mainly consisting in the presence of well-developed stress fibers and membrane ruffles. The interaction of CNF1 wit...

journal_title：Cytotechnology

authors： Falzano L,Fiorentini C,Boquet P,Donelli G

更新日期：1993-01-01 00:00:00