Abstract:
:A water soluble extract from the bark of the Samoan medicinal plant Alphitonia zizyphoides A. Gray (Rhamnaceae), enhances the plating efficiency in vitro of lymphoid cell lines as well as the survival of bone marrow cells and normal T and B lymphocytes. Furthermore, the inclusion of bark-extract into culture media enhances the cloning efficiency of a T-hybridoma cell line by more than 30 times at otherwise unsuitably low serum concentrations, but does not completely substitute for serum. The enhanced growth of a B-cell hybridoma is also paralleled by an increased production of monoclonal antibodies in cultures containing low cell densities.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Dunstan CA,Andersson J,Bohlin L,Cox PA,Grönvik KOdoi
10.1007/BF00772193subject
Has Abstractpub_date
1994-01-01 00:00:00pages
27-38issue
1eissn
0920-9069issn
1573-0778journal_volume
14pub_type
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