Binucleated HeLa cells are formed by cytokinesis failure in starvation and keep the potential of proliferation.

abstract：:Matrine is a bioactive component of the traditional Chinese medical herb Sophora flavescens that has been used in China to treat various kinds of diseases including virus hepatitis. However, the molecular mechanisms underlying its hepatoprotective effects remains elusive. In the present study, primary human hepatocyte...

journal_title：Cytotechnology

authors： Gong X,Gao Y,Guo G,Vondran FW,Schwartlander R,Efimova E,Pless G,Sauera IM,Neuhaus P

更新日期：2015-03-01 00:00:00

abstract：:The effects of media concentrations of glucose andglutamine on the intracellular nucleotide pools andoxygen uptake rates of a murine antibody-secretinghybridoma cell line were investigated. Cells takenfrom mid-exponential phase of growth were incubated inmedium containing varying concentrations of glucose(0-25 mM) and...

journal_title：Cytotechnology

authors： Barnabé N,Butler M

更新日期：2000-10-01 00:00:00

abstract：:The subcellular localisation of doxorubicin and Victoria Blue BO (VBBO) in a murine mammary tumour cell line EMT6-S, and the resistant sub-lineEMT6-R was studied, using confocal microscopy, in order to investigate their sites of action. In cells treated with doxorubicin (10 mu M) for 90 min, the pattern of intracellul...

journal_title：Cytotechnology

authors： Burrow SM,Phoenix DA,Wainwright M,Tobin MJ

更新日期：2002-05-01 00:00:00

abstract：:The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing cancer-initiating cells with stem cell properties at the single cell level has proven technically difficult because of the scarcity of the cancer ste...

journal_title：Cytotechnology

authors： Cocola C,Sanzone S,Astigiano S,Pelucchi P,Piscitelli E,Vilardo L,Barbieri O,Bertoli G,Reinbold RA,Zucchi I

更新日期：2008-09-01 00:00:00

abstract：:NS0 has been used as a fusion partner for the production of hybridomas and has more recently been engineered to produce recombinant protein. A protein-free culture medium, designated W38 medium, has previously been developed which supported high density growth of rat myeloma and hybridoma cell lines. NS0 cells failed ...

journal_title：Cytotechnology

authors： Keen MJ,Steward TW

更新日期：1995-10-01 00:00:00

abstract：:There is a constant and growing interest in exploitingadenoviruses as vectors for gene therapy when transientexpression of a therapeutic protein is necessary. Therequirement for an increased viral titre has prompted asearch for techniques by which this virus may be assayedwith greater speed and simplicity. Conventiona...

journal_title：Cytotechnology

authors： Gueret V,Negrete-Virgen JA,Lyddiatt A,Al-Rubeai M

更新日期：2002-01-01 00:00:00

abstract：:The effect of oxygen partial pressure on viral replication was investigated with Vero/VSV system. At 10% oxygen partial pressure in spinner culture, VSV titer was significantly increased 130 fold compared to that obtained at 21%. A similar result was obtained for viral production in 1liter bioreactor. This implies tha...

journal_title：Cytotechnology

authors： Lim HS,Chang KH,Kim JH

更新日期：1999-11-01 00:00:00

abstract：:Despite the fact, that monoclonal antibodies are the fastest growing group of biopharmaceuticals in development, this is not true for the IgM class, which remains as enigmatic as ever. While more examples of usefulness of IgMs for medical applications are emerging, their recombinant production is still not common. In ...

journal_title：Cytotechnology

authors： Chromikova V,Mader A,Steinfellner W,Kunert R

更新日期：2015-03-01 00:00:00

abstract：:Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, mo...

journal_title：Cytotechnology

authors： Wu JY,Sheikho A,Ma H,Li TC,Zhao YQ,Zhang YL,Wang D

更新日期：2017-12-01 00:00:00

abstract：:The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate wit...

journal_title：Cytotechnology

authors： Tanaka H,Fukuda N,Shoyama Y

更新日期：1999-03-01 00:00:00

abstract：:The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...

journal_title：Cytotechnology

authors： Shoyama Y,Tanaka H,Fukuda N

更新日期：1999-09-01 00:00:00

abstract：:The Baculovirus Expression Vector System has become widely used for the production of recombinant proteins for research and diagnostics. Serum-free culture media able to support high cell densities have been developed for the large scale culture of insect cells. While serum elimination aims at avoiding the risks assoc...

journal_title：Cytotechnology

authors： Deparis V,Durrieu C,Schweizer M,Marc I,Goergen JL,Chevalot I,Marc A

更新日期：2003-07-01 00:00:00

abstract：:Replication Kinetics of HIV was studied under single-growth cycle conditions in C8166 lymphoblastoid cells by back-titrating released and cell-associated infectious virus et each time point. Under these conditions HIV seemed to replicate faster than previously estimated. The amount cell-associated virus always exceede...

journal_title：Cytotechnology

authors： Dianzani F,Antonelli G

更新日期：1991-02-01 00:00:00

abstract：:Dental pulp stem cells constitute an attractive source of multipotent mesenchymal stem cells owing to their high proliferation rate and multilineage differentiation potential. Osteogenesis is initiated by osteoblasts, which originate from mesenchymal stem cells. These cells express specific surface antigens that disap...

journal_title：Cytotechnology

authors： Mortada I,Mortada R

更新日期：2018-10-01 00:00:00

abstract：:A promising method for diabetes treatment is the implantation of immunoisolated cells secreting insulin in response to glucose. Cell availability limits the application of this approach at a medically-relevant scale. We explore the use of transformed cells that can be grown to large homogeneous populations in developi...

journal_title：Cytotechnology

authors： Sambanis A,Papas KK,Flanders PC,Long RC Jr,Kang H,Constantinidis I

更新日期：1994-01-01 00:00:00

abstract：:"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...

journal_title：Cytotechnology

authors： Dudek D

更新日期：1994-01-01 00:00:00

abstract：:The application of image analysis methods to conventional thin sections for electron microscopy to analyze the chromatin arrangement are quite limited. We developed a method which utilizes freeze-fractured samples; the results indicate that the method is suitable for identifying the changes in the chromatin arrangemen...

journal_title：Cytotechnology

authors： Maraldi NM,Marinelli F,Squarzoni S,Santi S,Barbieri M

更新日期：1991-02-01 00:00:00

abstract：:For the mouse hybridoma cell line VO 208, kinetics of growth, consumption of glucose and glutamine, and production of lactate, ammonia and antibodies were compared in batch and continuous cultures. At a given specific growth rate, different metabolic activities were observed: a 40% lower glucose and glutamine consumpt...

journal_title：Cytotechnology

authors： Goergen JL,Marc A,Engasser JM

更新日期：1992-01-01 00:00:00

abstract：:Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and ...

journal_title：Cytotechnology

authors： Azimi M,Niimi T,Yoshida N,Kitagawa Y

更新日期：1999-09-01 00:00:00

abstract：:The feasibility of using genipin cross-linked type II collagen scaffold with rabbit bone marrow mesenchymal stem cells (RBMSCs) to repair cartilage defect was herein studied. Induction of RBMSCs into chondrocytic phenotype on type II collagen scaffold in vitro was conducted using TGF-β 3 containing medium. After 3-wee...

journal_title：Cytotechnology

authors： Chen WC,Yao CL,Wei YH,Chu IM

更新日期：2011-01-01 00:00:00

abstract：:Photopolymerizable hydrogels offer great potential in cartilage tissue engineering due to their ability to conform to irregular defect shapes and be applied in a potentially minimally invasive manner. An important process requirement in the use of photopolymerizable hydrogels is the ability of the suspended cells to w...

journal_title：Cytotechnology

authors： Dua R,Ramaswamy S

更新日期：2013-08-01 00:00:00

abstract：:The degranulation of basophils stimulated by antigen is one of the allergic mechanisms of immediate hypersensitivity reactions. In a previous study, the β-hexosaminidase release of rat basophilic leukemia (RBL-2H3) cells stimulated by IgE cross-linking was specifically inhibited by ethanol extract of olive milled wast...

journal_title：Cytotechnology

authors： Kishikawa A,Amen Y,Shimizu K

更新日期：2017-04-01 00:00:00

abstract：:The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...

journal_title：Cytotechnology

authors： Wikarta JM,Kim SM

更新日期：2016-08-01 00:00:00

abstract：:By employing the affinity gel fraction technique, we have detected a 175 kDa tyrosine-O-sulfate (TyrS)-binding protein in sodium choleate extracts of the microsomal membrane fractions of bovine liver and pancreas, as well as canine liver and pancreas. Western blot analysis revealed the presence of the bovine liver Tyr...

journal_title：Cytotechnology

authors： Liu MC,Sakakibara Y,Suiko M

更新日期：1997-01-01 00:00:00

abstract：:Monoclonal antibody (McAb) has been established as one of the most successful therapeutic strategies for the treatment of cancer. M1A2 (McAb) as a new monoclonal antibody was designed to recognize heat shock protein (HSP60), but its optimum production condition has not been studied. In this study, the cell culture con...

journal_title：Cytotechnology

authors： Bashokouh F,Abbasiliasi S,Tan JS

更新日期：2019-07-16 00:00:00

abstract：:Hepatocytes self-assemble in culture to form compacted spherical aggregates, or spheroids, that mimic the structure of the liver by forming tight junctions and bile canalicular channels. Hepatocyte spheroids thus resemble the liver to a great extent. However, liver tissue contains other cell types and has bile ducts a...

journal_title：Cytotechnology

authors： Abu-Absi SF,Hansen LK,Hu WS

更新日期：2004-07-01 00:00:00

abstract：:The bone marrow represents the most common source from which to isolate mesenchymal stem cells (MSCs). MSCs are capable of differentiating into tissues of the three primary lineages and have the potential to enhance repair in damaged organs through the principals of regenerative medicine. Given the ease with which MSC...

journal_title：Cytotechnology

authors： Dickinson H,Milton P,Jenkin G

更新日期：2012-10-01 00:00:00

abstract：:Daphnoretin is a bicoumarin compound isolated from a natural product, Wikstroemia indica, which has been used to treat many diseases. It has strong antiviral and anti-tumor activities. Taking the anti-tumor activity of daphnoretin as a starting point, the present study aimed to test the pro-apoptotic effect of daphnor...

journal_title：Cytotechnology

authors： Yang ZY,Kan JT,Cheng ZY,Wang XL,Zhu YZ,Guo W

更新日期：2014-01-01 00:00:00

abstract：:A hallmark of human cancer cells is immortal cell growth, which is associated with telomere maintenance by telomerase. The transcriptional regulation of the human telomerase reverse transcriptase (hTERT) gene is a major mechanism that negatively and positively controls telomerase activity in normal and cancer cells, r...

journal_title：Cytotechnology

authors： Horikawa I,Michishita E,Barrett JC

更新日期：2004-06-01 00:00:00

abstract：:To determine the function of miR-206 in epilepsy. Epileptic rat model was established by intra-amygdala injection of kainic acid (KA). Expression levels of miR-206, C-C Motif Chemokine Ligand 2 (CCL2) and interleukin-1β (Il-1β) in hippocampus tissues was measured by reverse transcription-quantitative PCR (RT-qPCR) and...

journal_title：Cytotechnology

authors： Wu Z,Liu Y,Huang J,Huang Y,Fan L

更新日期：2019-06-26 00:00:00