Abstract:
:Achyranthes bidentata (A. bidentata) Blume is a medicinal herb with the property of strengthening bones and muscles and ensuring proper downward flow of blood in terms of the therapeutic theory of traditional medicine. In the present study, the effect of A. bidentata root extract (AE) on osteoblast function was investigated in osteoblastic MC3T3-E1 cells. AE caused a significant elevation of alkaline phosphatase activity, collagen synthesis, osteocalcin production, and mineralization in the cells (P < 0.05). AE also decreased the production of TNF-α, IL-6, and RANKL induced by antimycin A, mitochondrial electron transport inhibitor. Exposure of MC3T3-E1 cells to antimycin A caused significant reduction of cell viability and mineralization. However, pretreatment with AE prior to antimycin A exposure significantly reduced antimycin A-induced cell damage by preventing mitochondrial membrane potential dissipation, ATP loss, ROS release, and nitrotyrosine increase, suggesting that AE may be useful for protecting mitochondria against a burst of oxidative stress. Moreover, AE increased the phosphorylation of cAMP-response element-binding protein inhibited by antimycin A. Our study demonstrates that A. bidentata could significantly prevent osteoblast damage in aged patients.
journal_name
Cytotechnologyjournal_title
Cytotechnologyauthors
Suh KS,Lee YS,Choi EMdoi
10.1007/s10616-013-9645-4subject
Has Abstractpub_date
2014-12-01 00:00:00pages
925-35issue
6eissn
0920-9069issn
1573-0778journal_volume
66pub_type
杂志文章相关文献
CYTOTECHNOLOGY文献大全abstract::Dental pulp stem cells (DPSCs), one type of mesenchymal stem cells, are considered to be a type of tool cells for regenerative medicine and tissue engineering. Our previous studies found that the stimulation with lipopolysaccharide (LPS) might introduce senescence of DPSCs, and this senescence would have a positive co...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0180-6
更新日期:2018-06-01 00:00:00
abstract::The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008149815908
更新日期:2000-03-01 00:00:00
abstract::The ratio of hapten to bovine serum albumin (BSA) in an antigen conjugate was determined by matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry. A hybridoma secreting monoclonal antibody (MAb) was produced by fusing splenocytes immunized with an antigen-BSA conjugate with HAT-sensitive mouse myel...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008051618059
更新日期:1999-09-01 00:00:00
abstract::Light scattering properties of hybridoma cells were examined with flow cytometry. Viable and dead cells form two distinct populations. The distribution of the two populations changes during a batch culture. the concentration of dead cells measured by flow cytometry correlates well to that measured by hemacytometer. Th...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00386140
更新日期:1989-05-01 00:00:00
abstract::Lentiviruses are quite effective gene delivery systems for stable production of genetically engineered human cells. However, prior to using lentivirus to deliver genetic materials to cells of interest, the normal course of production of these lentiviruses involves a lengthy collection, purification, preservation, and ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00343-0
更新日期:2019-10-01 00:00:00
abstract::We found that the formation of multilayer rosettes by transformed human blood lymphocytes after phytohemagglutinin (PHA) stimulation is correlated with conformational changes of the chromatin as seen by premature chromosome condensation (PCC). The frequency distribution of grades of PCC and multilayer rosette formatio...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00145027
更新日期:1988-07-01 00:00:00
abstract::Cells derived from the periodontal ligament (PDL) have previously been reported to have stem cell-like characteristics (PDL stem cells; PDLSCs) and play an important part in bone engineering, including that of alveolar bone. However, these populations have been heterogeneous, and thus far no specific marker has yet be...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-011-9390-5
更新日期:2012-01-01 00:00:00
abstract::Human herpes virus 6 (HHV-6) is a common human pathogen that is most often detected in hematopoietic cells. Although human cells harboring chromosomally integrated HHV-6 can be generated in vitro, the availability of such cell lines originating from in vivo tissues is limited. In this study, chromosomally integrated H...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-017-0119-y
更新日期:2018-02-01 00:00:00
abstract::The Korean horse mussel extract was purified and fractionated by a bioassay-guided purification step. The final fraction contained seven steroid and one polycyclic aromatic compounds, in which cholest-7-en-3-ol, (3β,5α)- (58.7 %) was a main component followed by ergosta-7,22dien-3-ol (3β,5α,22E) (13.0 %). This extract...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-014-9840-y
更新日期:2016-08-01 00:00:00
abstract::Vasculogenesis, the formation of blood vessels in embryonic or fetal tissue mediated by immature vascular cells (i.e., angioblasts), is poorly understood. Here we report a summary of our recent studies on the identification of a population of vascular progenitor cells (VPCs) in human fetal aorta. These undifferentiate...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-008-9167-7
更新日期:2008-09-01 00:00:00
abstract::Toxicity testing is required for new chemicals being introduced onto the market. The use of animals in evaluating chemical safety is costly and time consuming. Furthermore, there is the ethical need to develop alternative methods to reduce the required number of animals. The new in vitro assays offer numerous advantag...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 famil...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-016-9995-9
更新日期:2016-12-01 00:00:00
abstract::Prostacyclin alternatively called prostaglandin (PG) I2 is an unstable metabolite synthesized by the arachidonate cyclooxygenase pathway. Earlier studies have suggested that prostacyclin analogues can act as a potent effector of adipose differentiation. However, biosynthesis of PGI2 has not been determined comprehensi...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-013-9616-9
更新日期:2014-08-01 00:00:00
abstract::We screened for biological activity which induces neurite outgrowth in vitro from 300 species of marine algae from along the Japan coast for possible use as a treatment for the lack of neurotrophic factor which is considered to be a cause of Alzheimer's disease. In this study, we evaluated the neurite outgrowth promot...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1023974323807
更新日期:2002-11-01 00:00:00
abstract::This paper reviews the JRC-Ispra research activity on toxicological studies related to the environmental and occupational exposure of trace metals, as carried out by a combination of cell culture methodologies with nuclear and radioanalytical techniques. Applications concern the setting of uptake-effect relationships ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::We describe a novel fully automated high-throughput time-lapse microscopy system and evaluate its performance for precisely tracking the motility of several glioma and osteoblastic cell lines. Use of this system revealed cell motility behavior not discernable with conventional techniques by collecting data (1) from cl...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9006-7
更新日期:2006-05-01 00:00:00
abstract::In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the firs...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-006-9015-6
更新日期:2006-06-01 00:00:00
abstract::"In vitro" studies, commonly using porcine ovarian follicles, may generate inconsistent results when atretic follicles are not eliminated from the pool of experimental follicles. The present experiment was conducted to test the practical value of the macroscopie identification of large porcine follicles, which were as...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00758179
更新日期:1994-01-01 00:00:00
abstract::In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-018-0206-8
更新日期:2018-08-01 00:00:00
abstract::Several lichen species have been used for medicinal purposes throughout the ages, and they are reported to be effective in the treatment of different disorders including ulcer and cancer. It is revealed that lichens may be easily accessible sources of natural drugs and possible food supplements after their safety eval...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-012-9447-0
更新日期:2012-12-01 00:00:00
abstract::Alkylating agents are the most widely used anticancer drugs whose main target is the DNA, although how exactly the DNA lesions cause cell death is still not clear. The emergence of resistance to this class of drugs as well as to other antitumor agents is one of the major causes of failure of cancer treatment. This pap...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1008060720608
更新日期:1998-09-01 00:00:00
abstract::Specific respiration rate ( [Formula: see text]) is a key parameter to understand cell metabolism and physiological state, providing useful information for process supervision and control. In this work, we cultivated different insect cells in a very controlled environment, being able to measure [Formula: see text]. Sp...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9118-8
更新日期:2008-05-01 00:00:00
abstract::The establishment of a cell culture system promoting chondrocyte differentiation has been utilized to better characterize phenotypic stages of chondrogenesis at the cellular level. Although the expression of the type II collagen gene has been studied during "in vitro" chondrocyte differentiation, little is known about...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00736804
更新日期:1991-02-01 00:00:00
abstract::A human continuous cell line (huGK-14) within a lineage of passaged cultures was investigated in the mode of integration and expression of hepatitis B virus (HBV) genes. HBV DNA was integrated in eight different sites of the cellular DNA, in each of which HBV genome was rearranged, fragmented, and/or partly deleted. C...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1007924119018
更新日期:1997-11-01 00:00:00
abstract::We provide evidence that the expression of the human MRP/GS-X pump encoded by the MRP (multidrug resistance associated protein) gene is induced by cisplatin in human leukemia HL-60/R-CP (cisplatin-resistant) cells and modulates cell growth inhibition by delta(7)-prostaglandin A1 (PGA1) methyl ester. The MRP mRNA level...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF00744216
更新日期:1996-01-01 00:00:00
abstract::Oxidative stress plays a vital role in the occurrence and development of intestinal injury. Soluble epoxide hydrolase 2 gene (EPHX2) is a class of hydrolytic enzymes. We aim to explore the effects and molecular mechanism of siEPHX2 on H2O2-induced oxidative damage in rat intestinal epithelial IEC-6 cells. IEC-6 cells ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-019-00354-x
更新日期:2020-02-01 00:00:00
abstract::Carvacrol, the predominant monoterpene in many essential oils of Labitae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus has substantial antibacterial, antifungal, antihelmintic, insecticial, analgesic and antioxidant activities. Approximately 75% of lung cancer is non-small cell carcinoma (NSCLC) whic...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/b:cyto.0000039917.60348.45
更新日期:2003-11-01 00:00:00
abstract::A Xenopus laevis peptidyl C-terminal alpha-amidating enzyme (AE-II) gene, modified by deletion of a region encoding the putative membrane-spanning domain and the putative C-terminal cytosolic tail, was expressed in BoMo-15 AIIc insect cells and silkworm larvae using a Bombyx mori baculovirus expression vector system. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/BF02525492
更新日期:1992-01-01 00:00:00
abstract::Fishes have been recently recognized as a suitable model organism to study vertebrate physiological processes, in particular skeletal development and tissue mineralization. However, there is a lack of well characterized in vitro cell systems derived from fish calcified tissues. We describe here a protocol that was suc...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1007/s10616-007-9098-8
更新日期:2007-09-01 00:00:00
abstract::To characterize the effects of cetyltriethylammonium bromide (CTAB) on the transcription of gp64 promoter from Bombyx mori nucleopolyhedrovirus (BmNPV), the plasmid pBmgp64Luc used in transient expression assay system was constructed by using the luciferase gene as a reporter under the control of BmNPV gp64 promoter. ...
journal_title:Cytotechnology
pub_type: 杂志文章
doi:10.1023/A:1024231023015
更新日期:2003-01-01 00:00:00